Strain identifier

BacDive ID: 140490

Type strain: Yes

Species: Actinomadura montaniterrae

Strain Designation: CYP1-1B

Culture col. no.: JCM 16995, KCTC 39784, PCU 349, TISTR 2400

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General

@ref: 43102

BacDive-ID: 140490

keywords: gram-positive, spore-forming, aerobe, Bacteria, 16S sequence, genome sequence

description: Actinomadura montaniterrae CYP1-1B is an aerobe, spore-forming, gram-positive bacterium that builds a substrate mycelium and was isolated from mountain soil sample.

doi: 10.13145/bacdive140490.20201210.5

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinobacteria
  • domain: Bacteria
  • phylum: Actinobacteria
  • class: Actinobacteria
  • order: Streptosporangiales
  • family: Thermomonosporaceae
  • genus: Actinomadura
  • species: Actinomadura montaniterrae
  • full scientific name: Actinomadura montaniterrae Songsumanus et al. 2016

@ref: 43102

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Streptosporangiales

family: Thermomonosporaceae

genus: Actinomadura

species: Actinomadura montaniterrae

strain designation: CYP1-1B

type strain: yes

Morphology

cell morphology

  • @ref: 43102
  • gram stain: positive

colony morphology

  • @ref: 43102
  • colony color: dark greyish yellow
  • medium used: ISP 2

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium namefurther description
43102yessubstrate myceliumdark greyish yellowISP2extensively branched non-fragmenting substrate mycelium
43102yesaerial myceliumpale greenISP2

Culture and growth conditions

culture medium

@refnamegrowth
43102ISP 7yes
43102nutritient agar (NA)yes
43102ISP 2yes

culture temp

  • @ref: 43102
  • growth: positive
  • type: growth
  • temperature: 20-45

culture pH

  • @ref: 43102
  • ability: positive
  • type: growth
  • pH: 4.5-8.0

Physiology and metabolism

oxygen tolerance

  • @ref: 43102
  • oxygen tolerance: aerobe

spore formation

  • @ref: 43102
  • spore description: oval to ellipsoid spores formed along the sporogenous hyphae and its branches
  • spore formation: yes

halophily

  • @ref: 43102
  • salt: NaCl
  • growth: positive
  • tested relation: maximum
  • concentration: 5 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4310217634D-glucose+carbon source
4310216899D-mannitol+carbon source
4310262345L-rhamnose+carbon source
4310217716lactose+carbon source
4310230849L-arabinose+carbon source
4310215824D-fructose+carbon source
4310217057cellobiose+carbon source
4310217992sucrose+carbon source
4310265327D-xylose+carbon source
4310212936D-galactose-carbon source
4310217754glycerol-carbon source
4310228053melibiose-carbon source
4310216634raffinose-carbon source
4310216988D-ribose-carbon source
4310217814salicin-carbon source
4310228017starch+hydrolysis
431025291gelatin-hydrolysis
4310217632nitrate-reduction
43102milk-hydrolysis

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    43102C14:04.6
    43102C16:031.2
    43102C17:00.5
    43102C18:03.3
    43102C16:1ω7c5.4
    43102C18:1ω9c17.1
    43102C16:0 10-methyl1.9
    43102C18:0 10-methyl19
    43102C16:0 2OH10.5
  • type of FA analysis: whole cell analysis
  • incubation medium: ISP 2
  • agar/liquid: liquid
  • incubation temperature: 30
  • incubation time: 7
  • software version: Sherlock 6.0
  • system: MIS MIDI

Isolation, sampling and environmental information

isolation

  • @ref: 43102
  • sample type: mountain soil sample
  • geographic location: Chaiyaphum Province
  • country: Thailand
  • origin.country: THA
  • continent: Asia
  • enrichment culture: starch casein nitrate agar
  • enrichment culture composition: supplemented with nystatin (50 mg/l) and nalidixic acid (20 mg/l)
  • enrichment culture duration: 21 days
  • enrichment culture temperature: 30

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Sequence information

16S sequences

  • @ref: 43102
  • description: Actinomadura sp. CYP1-1B gene for 16S ribosomal RNA, partial sequence
  • accession: LC126428
  • length: 1488
  • database: nuccore
  • NCBI tax ID: 1803903

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Actinomadura montaniterrae CYP1-1BGCA_008923365scaffoldncbi1803903
66792Actinomadura montaniterrae strain CYP1-1B1803903.3wgspatric1803903

GC content

  • @ref: 43102
  • GC-content: 74.3
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 43102

culture collection no.: JCM 16995, KCTC 39784, PCU 349, TISTR 2400

Reference

@idauthorstitledoi/urljournal
20215D.Gleim, M.Kracht, N.Weiss et. al.Prokaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes sincehttp://www.dsmz.de/bacterial-diversity/prokaryotic-nomenclature-up-to-date.html
43102Apakorn Songsumanus, Takuji Kudo, Moriya Ohkuma, Wongsakorn Phongsopitanun, Somboon TanasupawatActinomadura montaniterrae sp. nov., isolated from mountain soil10.1099/ijsem.0.001196IJSEM 66: 3310-3316 2016
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes