Strain identifier
BacDive ID: 140473
Type strain:
Species: Aquaspirillum soli
Strain Designation: THG-SQE6
Strain history: T.-H. Yi; Kyung Hee Univ., South Korea; THG-SQE6.
NCBI tax ID(s): 1591092 (species)
General
@ref: 43221
BacDive-ID: 140473
keywords: 16S sequence, Bacteria, microaerophile, Gram-negative, motile, helical-shaped, colony-forming
description: Aquaspirillum soli THG-SQE6 is a microaerophile, Gram-negative, motile bacterium that forms circular colonies and was isolated from soil sample of a reed pond.
NCBI tax id
- NCBI tax id: 1591092
- Matching level: species
strain history
- @ref: 67770
- history: T.-H. Yi; Kyung Hee Univ., South Korea; THG-SQE6.
doi: 10.13145/bacdive140473.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Betaproteobacteria
- order: Neisseriales
- family: Chromobacteriaceae
- genus: Aquaspirillum
- species: Aquaspirillum soli
- full scientific name: Aquaspirillum soli Moya et al. 2017
@ref: 43221
domain: Bacteria
phylum: Proteobacteria
class: Betaproteobacteria
order: Neisseriales
family: Chromobacteriaceae
genus: Aquaspirillum
species: Aquaspirillum soli
strain designation: THG-SQE6
type strain: yes
Morphology
cell morphology
- @ref: 43221
- gram stain: negative
- cell length: 5.3-9.3 µm
- cell width: 0.9-1.1 µm
- cell shape: helical-shaped
- motility: yes
colony morphology
- @ref: 43221
- colony size: 1.0-2.0 mm
- colony color: opaque white
- colony shape: circular
- incubation period: 3-4 days
- medium used: R2A
Culture and growth conditions
culture medium
@ref | name | growth |
---|---|---|
43221 | Reasoner's 2A agar (R2A) | yes |
43221 | TSA (tryptic soy agar) | yes |
43221 | nutritient agar (NA) | yes |
43221 | marine agar (MA) | no |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
43221 | positive | optimum | 25-42 | |
43221 | positive | growth | 4-42 | |
67770 | positive | growth | 28 | mesophilic |
culture pH
@ref | ability | type | pH | PH range |
---|---|---|---|---|
43221 | positive | growth | 6-8.5 | alkaliphile |
43221 | positive | optimum | 6.5-7.5 |
Physiology and metabolism
oxygen tolerance
- @ref: 43221
- oxygen tolerance: microaerophile
halophily
- @ref: 43221
- salt: NaCl
- growth: positive
- tested relation: growth
- concentration: 0-1.5 %(w/v)
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
43221 | 53424 | tween 20 | - | hydrolysis |
43221 | 53426 | tween 80 | - | hydrolysis |
43221 | 17895 | L-tyrosine | - | hydrolysis |
43221 | casein | - | hydrolysis | |
43221 | 17029 | chitin | - | hydrolysis |
43221 | 28017 | starch | - | hydrolysis |
43221 | 85146 | carboxymethylcellulose | - | hydrolysis |
43221 | 16991 | dna | - | hydrolysis |
43221 | 17632 | nitrate | + | reduction |
43221 | 17234 | glucose | + | builds acid from |
43221 | 4853 | esculin | + | hydrolysis |
43221 | 25115 | malate | + | assimilation |
43221 | 506227 | N-acetylglucosamine | + | assimilation |
43221 | 17634 | D-glucose | + | assimilation |
43221 | 30849 | L-arabinose | - | assimilation |
43221 | 16899 | D-mannitol | - | assimilation |
43221 | 16024 | D-mannose | - | assimilation |
43221 | 17306 | maltose | - | assimilation |
43221 | 24265 | gluconate | - | assimilation |
43221 | 27689 | decanoate | - | assimilation |
43221 | 17128 | adipate | - | assimilation |
43221 | 53258 | sodium citrate | - | assimilation |
43221 | 18401 | phenylacetate | - | assimilation |
43221 | 17814 | salicin | + | assimilation |
43221 | 17272 | propionate | + | assimilation |
43221 | 31011 | valerate | + | assimilation |
43221 | 37054 | 3-hydroxybutyrate | + | assimilation |
43221 | 17203 | L-proline | + | assimilation |
43221 | 32954 | sodium acetate | + | assimilation |
43221 | 16977 | L-alanine | + | assimilation |
43221 | 17115 | L-serine | + | assimilation |
43221 | 24996 | lactate | + | assimilation |
43221 | 28053 | melibiose | - | assimilation |
43221 | 18287 | L-fucose | - | assimilation |
43221 | 17924 | D-sorbitol | - | assimilation |
43221 | 15971 | L-histidine | - | assimilation |
43221 | 16808 | 2-dehydro-D-gluconate | - | assimilation |
43221 | 17879 | 4-hydroxybenzoate | - | assimilation |
43221 | 62345 | L-rhamnose | - | assimilation |
43221 | 16988 | D-ribose | - | assimilation |
43221 | 17268 | myo-inositol | - | assimilation |
43221 | 17992 | sucrose | - | assimilation |
43221 | 17240 | itaconate | - | assimilation |
43221 | 9300 | suberic acid | - | assimilation |
43221 | 62983 | sodium malonate | - | assimilation |
43221 | 58143 | 5-dehydro-D-gluconate | - | assimilation |
43221 | 28087 | glycogen | - | assimilation |
43221 | 16193 | 3-hydroxybenzoate | - | assimilation |
metabolite production
- @ref: 43221
- Chebi-ID: 35581
- metabolite: indole
- production: no
enzymes
@ref | value | activity | ec |
---|---|---|---|
43221 | catalase | + | 1.11.1.6 |
43221 | cytochrome oxidase | + | 1.9.3.1 |
43221 | alkaline phosphatase | + | 3.1.3.1 |
43221 | esterase (C 4) | + | |
43221 | esterase Lipase (C 8) | + | |
43221 | leucine arylamidase | + | 3.4.11.1 |
43221 | valine arylamidase | + | |
43221 | cystine arylamidase | + | 3.4.11.3 |
43221 | beta-glucosidase | + | 3.2.1.21 |
43221 | acid phosphatase | + | 3.1.3.2 |
43221 | trypsin | + | 3.4.21.4 |
43221 | lipase (C 14) | - | |
43221 | chymotrypsin | - | 3.4.4.5 |
43221 | naphthol-AS-BI-phosphohydrolase | - | |
43221 | alpha-galactosidase | - | 3.2.1.22 |
43221 | beta-galactosidase | - | 3.2.1.23 |
43221 | beta-glucuronidase | - | 3.2.1.31 |
43221 | alpha-glucosidase | - | 3.2.1.20 |
43221 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
43221 | alpha-mannosidase | - | 3.2.1.24 |
43221 | alpha-fucosidase | - | 3.2.1.51 |
43221 | arginine dihydrolase | - | 3.5.3.6 |
fatty acid profile
fatty acids
@ref fatty acid percentage 43221 C10:0 1.2 43221 C14:0 1.4 43221 C16:0 21.3 43221 C18:0 5.3 43221 C18:3ω6,9,12c 1.5 43221 C12:0 anteiso 1.5 43221 C12:0 3OH 10.8 43221 C16:1ω7c / C16:1ω6c 44.7 43221 C18:1ω7c 8.9 - type of FA analysis: whole cell analysis
- incubation medium: R2A
- agar/liquid: liquid
- incubation temperature: 28
- incubation time: 2
- system: MIS MIDI
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent | latitude | longitude | enrichment culture | enrichment culture duration | enrichment culture temperature | isolation procedure |
---|---|---|---|---|---|---|---|---|---|---|---|
43221 | soil sample of a reed pond | Shangqiu, Henan province | China | CHN | Asia | 34 | 115 | nutrient agar (NA, Difco) | 1 week | 28 | serial dilution plating method |
67770 | Soil of a reed pond in Shangqiu | Henan Province | China | CHN | Asia |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Aquatic | #Pond (small) |
#Environmental | #Terrestrial | #Soil |
Sequence information
16S sequences
- @ref: 43221
- description: Aquaspirillum sp. THG-SQE6 16S ribosomal RNA gene, partial sequence
- accession: KM598238
- length: 1453
- database: nuccore
- NCBI tax ID: 1591092
GC content
- @ref: 67770
- GC-content: 53.1
- method: high performance liquid chromatography (HPLC)
External links
@ref: 43221
culture collection no.: CCTCC AB 2016081, KACC 18846, JCM 30429
literature
- topic: Phylogeny
- Pubmed-ID: 28109204
- title: Aquaspirillum soli sp. nov., isolated from a soil sample.
- authors: Moya G, Trinh H, Yan ZF, Won KH, Yang JE, Wang QJ, Kook MC, Yi TH
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijsem.0.001802
- year: 2017
- mesh: Bacterial Typing Techniques, Base Composition, Betaproteobacteria/*classification/genetics/isolation & purification, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Ubiquinone/chemistry
- topic2: Transcriptome
Reference
@id | authors | title | doi/url | journal | pubmed |
---|---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | ||
43221 | Gabriela Moya, Huan Trinh, Zheng-Fei Yan, Kyung-Hwa Won, Jung-Eun Yang, Qi-Jun Wang, Moo-Chang Kook, Tae-Hoo Yi | Aquaspirillum soli sp. nov., isolated from a soil sample | 10.1099/ijsem.0.001802 | IJSEM 67: 1312-1317 2017 | 28109204 |
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ |