Strain identifier

BacDive ID: 140459

Type strain: Yes

Species: Paenibacillus lacus

Strain Designation: Agd-32

Strain history: <- Wen-Ming Chen, National Kaohsiung Marine Univ.

NCBI tax ID(s): 1615953 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 8.1 (current version)

General

@ref: 43197

BacDive-ID: 140459

keywords: 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-variable, motile, rod-shaped, colony-forming

description: Paenibacillus lacus Agd-32 is an aerobe, spore-forming, mesophilic bacterium that forms circular colonies and was isolated from water.

NCBI tax id

  • NCBI tax id: 1615953
  • Matching level: species

strain history

  • @ref: 67771
  • history: <- Wen-Ming Chen, National Kaohsiung Marine Univ.

doi: 10.13145/bacdive140459.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Paenibacillaceae
  • genus: Paenibacillus
  • species: Paenibacillus lacus
  • full scientific name: Paenibacillus lacus Chen et al. 2017

@ref: 43197

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Caryophanales

family: Paenibacillaceae

genus: Paenibacillus

species: Paenibacillus lacus

strain designation: Agd-32

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityflagellum arrangement
43197variable3.0-4.0 µm0.5-1.0 µmrod-shapedyesperitrichous
67771rod-shapedyesperitrichous
67771variable

colony morphology

  • @ref: 43197
  • colony size: 0.5-2.0 mm
  • colony color: non-pigmented
  • colony shape: circular
  • incubation period: 2 days
  • medium used: R2A

Culture and growth conditions

culture medium

@refnamegrowth
43197Reasoner's 2A agar (R2A)yes
43197nutritient agar (NA)no
43197trypticase soy agar (TSA)no
43197Luria Bertani (LB) agarno

culture temp

@refgrowthtypetemperaturerange
43197positiveoptimum37mesophilic
43197positivegrowth25-40
67771positivegrowth30mesophilic

culture pH

@refabilitytypepH
43197positivegrowth7-8
43197positiveoptimum7

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
43197aerobe
67771aerobe

spore formation

  • @ref: 43197
  • spore description: ellipsoidal in swollen sporangia
  • type of spore: endospore
  • spore formation: yes

halophily

@refsaltgrowthtested relationconcentration
43197NaClpositivegrowth0-3 %
43197NaClpositiveoptimum2 %

observation

@refobservation
43197cells are surrounded by a thick capsule
67771quinones: MK-7

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4319785146carboxymethylcellulose+hydrolysis
431974853esculin+hydrolysis
431975291gelatin+hydrolysis
4319717029chitin-hydrolysis
43197casein-hydrolysis
4319728017starch-hydrolysis
4319716199urea-hydrolysis
4319716991dna-hydrolysis
43197corn oil-hydrolysis
4319758187alginate-hydrolysis
4319761995lecithin-hydrolysis
4319753424tween 20-hydrolysis
4319753423tween 40-hydrolysis
4319753425tween 60-hydrolysis
4319753426tween 80-hydrolysis
4319717632nitrate-reduction
4319717634D-glucose-builds acid from
4319717754glycerol+builds acid from
4319717108D-arabinose+
4319730849L-arabinose+
4319716988D-ribose+
4319765327D-xylose+
4319774863methyl beta-D-xylopyranoside+
4319712936D-galactose+
4319717634D-glucose+
4319715824D-fructose+
4319716024D-mannose+
4319762345L-rhamnose+
4319743943methyl alpha-D-mannoside+
43197320061methyl alpha-D-glucopyranoside+
431974853esculin+
4319717814salicin+
4319717057cellobiose+
4319717306maltose+
4319717716lactose+
4319728053melibiose+
4319716634raffinose+
4319728017starch+
4319728066gentiobiose+
4319716443D-tagatose+
4319718287L-fucose+
43197581435-dehydro-D-gluconate+
4319717266L-sorbose+builds acid from
4319718305arbutin+
4319762318D-lyxose+
4319717113erythritol-builds acid from
4319765328L-xylose-builds acid from
4319715963ribitol-builds acid from
4319716813galactitol-builds acid from
4319717268myo-inositol-builds acid from
4319716899D-mannitol-builds acid from
4319717924D-sorbitol-builds acid from
43197506227N-acetylglucosamine-builds acid from
4319727613amygdalin-builds acid from
4319717992sucrose-builds acid from
4319727082trehalose-builds acid from
4319715443inulin-builds acid from
431976731melezitose-builds acid from
4319728087glycogen-builds acid from
4319717151xylitol-builds acid from
4319732528turanose-builds acid from
4319728847D-fucose-builds acid from
4319718333D-arabitol-builds acid from
4319718403L-arabitol-builds acid from
4319724265gluconate-builds acid from
431972-oxogluconate-builds acid from

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.group IDis resistantresistance conc.
4319728971ampicillinyesyes10 µg (disc)
4319717698chloramphenicolyesyes30 µg (disc)
4319717833gentamicinyesyes10 µg (disc)
431976104kanamycinyesyes30 µg (disc)
43197100147nalidixic acidyesyes30 µg (disc)
4319728368novobiocinyesyes30 µg (disc)
4319718208penicillin gyesyes10 Unit (disc)
4319728077rifampicinyesyes5 µg (disc)
4319717076streptomycinyesyes10 µg (disc)
431979332sulfamethoxazoleyes26yes23.75 µg (disc)
4319727902tetracyclineyesyes30 µg (disc)
4319745924trimethoprimyes26yes1.25 µg (disc)

metabolite production

  • @ref: 43197
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
43197cytochrome oxidase+1.9.3.1
43197catalase+1.11.1.6
43197arginine dihydrolase-3.5.3.6
43197alkaline phosphatase+3.1.3.1
43197esterase (C 4)+
43197esterase Lipase (C 8)+
43197leucine arylamidase+3.4.11.1
43197valine arylamidase+
43197acid phosphatase+3.1.3.2
43197naphthol-AS-BI-phosphohydrolase+
43197alpha-galactosidase+3.2.1.22
43197beta-galactosidase+3.2.1.23
43197beta-glucuronidase+3.2.1.31
43197beta-glucosidase+3.2.1.21
43197lipase (C 14)-
43197cystine arylamidase-3.4.11.3
43197trypsin-3.4.21.4
43197alpha-chymotrypsin-3.4.21.1
43197alpha-glucosidase-3.2.1.20
43197N-acetyl-beta-glucosaminidase-3.2.1.52
43197alpha-mannosidase-3.2.1.24
43197alpha-fucosidase-3.2.1.51

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    43197C12:02.1
    43197C14:03.2
    43197C16:020.7
    43197C18:04.7
    43197C14:0 iso3.9
    43197C15:0 iso2
    43197C16:0 iso9.6
    43197C17:0 iso1.5
    43197C14:0 anteiso2.6
    43197C15:0 anteiso41.7
    43197C17:0 anteiso4.8
    43197C16:1ω11c1.2
  • type of FA analysis: whole cell analysis
  • incubation medium: R2A
  • agar/liquid: agar
  • incubation temperature: 30
  • incubation time: 2
  • software version: Sherlock 6.0
  • system: MIS MIDI
  • instrument: 5890 Series II, Hewlett-Packard
  • cutoff value:

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudeenrichment cultureenrichment culture durationenrichment culture temperature
43197waterAgongdian Reservoir, vicinity of Kaohsiung cityTaiwanTWNAsia22120R2A agar3 days25
67771From a water sample taken from the Agongdian Reservoir in the vicinity of Kaohsiung cityTaiwanTWNAsia

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic

taxonmaps

  • @ref: 69479
  • File name: preview.99_139162.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15358;96_37014;97_47048;98_61682;99_139162&stattab=map
  • Last taxonomy: Paenibacillus
  • 16S sequence: LN812815
  • Sequence Identity:
  • Total samples: 166
  • soil counts: 131
  • aquatic counts: 4
  • animal counts: 18
  • plant counts: 13

Sequence information

16S sequences

  • @ref: 43197
  • description: Paenibacillus sp. Agd-32 partial 16S rRNA gene, strain Agd-32
  • accession: LN812815
  • length: 1454
  • database: nuccore
  • NCBI tax ID: 1615953

GC content

@refGC-contentmethod
4319756high performance liquid chromatography (HPLC)
6777156.0

External links

@ref: 43197

culture collection no.: BCRC 80902, KCTC 33691, LMG 28836

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny28537545Paenibacillus lacus sp. nov., isolated from a water reservoir.Chen WM, Lin KR, Sheu SYInt J Syst Evol Microbiol10.1099/ijsem.0.0017682017Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Fresh Water/*microbiology, Glycolipids/chemistry, Paenibacillus/*classification/genetics/isolation & purification, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, TaiwanTranscriptome
Phylogeny29458566Paenibacillus translucens sp. nov., isolated from tidal flat sediment.Kim YS, Cha CJInt J Syst Evol Microbiol10.1099/ijsem.0.0026132018Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Geologic Sediments/*microbiology, Glycolipids/chemistry, Paenibacillus/*classification/genetics/isolation & purification, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Seawater/*microbiology, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistryTranscriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
43197Wen-Ming Chen, Kai-Rou Lin, Shih-Yi SheuPaenibacillus lacus sp. nov., isolated from a water reservoir10.1099/ijsem.0.001768IJSEM 67: 1582-1588 201728537545
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/