Strain identifier
BacDive ID: 140413
Type strain:
Species: Thermosulfuriphilus ammonigenes
Strain Designation: ST65
Strain history: <- G. Slobodkina, Winogradsky Inst. Microbiol., RAS, Moscow, Russia; ST65 <- G. Slobodkina {2013}
NCBI tax ID(s): 1936021 (species)
General
@ref: 43020
BacDive-ID: 140413
DSM-Number: 102941
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, thermophilic, motile
description: Thermosulfuriphilus ammonigenes ST65 is an anaerobe, thermophilic, motile bacterium that was isolated from deep-sea actively venting hydrothermal sulfidic chimney.
NCBI tax id
- NCBI tax id: 1936021
- Matching level: species
strain history
- @ref: 43020
- history: <- G. Slobodkina, Winogradsky Inst. Microbiol., RAS, Moscow, Russia; ST65 <- G. Slobodkina {2013}
doi: 10.13145/bacdive140413.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/thermodesulfobacteriota
- domain: Bacteria
- phylum: Thermodesulfobacteriota
- class: Thermodesulfobacteriota, not assigned to class
- order: Thermodesulfobacteriota, not assigned to order
- family: Thermodesulfobacteriota, not assigned to family
- genus: Thermosulfuriphilus
- species: Thermosulfuriphilus ammonigenes
- full scientific name: Thermosulfuriphilus ammonigenes Slobodkina et al. 2017
@ref: 43020
domain: Bacteria
phylum: Thermodesulfobacteria
class: Thermodesulfobacteria
order: Thermodesulfobacteria, not assigned to order
family: Thermodesulfobacteriaceae
genus: Thermosulfuriphilus
species: Thermosulfuriphilus ammonigenes
full scientific name: Thermosulfuriphilus ammonigenes Slobodkina et al. 2017
strain designation: ST65
type strain: yes
Morphology
cell morphology
@ref | motility | confidence | gram stain |
---|---|---|---|
69480 | yes | 90.947 | |
69480 | 99.979 | negative |
Culture and growth conditions
culture medium
- @ref: 43020
- name: THERMOSULFURIPHILUS MEDIUM (DSMZ Medium 1210c)
- growth: yes
- link: https://mediadive.dsmz.de/medium/1210c
- composition: Name: THERMOSULFURIPHILUS MEDIUM (DSMZ Medium 1210c) Composition: NaCl 17.9462 g/l Sulfur 4.98504 g/l MgCl2 x 6 H2O 3.98804 g/l Na2S2O3 x 5 H2O 3.48953 g/l Na2CO3 0.997009 g/l KNO3 0.997009 g/l (NH4)2SO4 0.498504 g/l CaCl2 x 2 H2O 0.329013 g/l KCl 0.329013 g/l KH2PO4 0.329013 g/l HCl 0.00249252 g/l FeCl2 x 4 H2O 0.00149551 g/l NaOH 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l Pyridoxine hydrochloride 9.97009e-05 g/l MnCl2 x 4 H2O 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l p-Aminobenzoic acid 4.98504e-05 g/l (DL)-alpha-Lipoic acid 4.98504e-05 g/l Calcium D-(+)-pantothenate 4.98504e-05 g/l Nicotinic acid 4.98504e-05 g/l Riboflavin 4.98504e-05 g/l Thiamine HCl 4.98504e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l Folic acid 1.99402e-05 g/l Biotin 1.99402e-05 g/l H3BO3 5.98205e-06 g/l Na2WO4 x 2 H2O 3.98804e-06 g/l Na2SeO3 x 5 H2O 2.99103e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Vitamin B12 9.97009e-07 g/l Distilled water
culture temp
@ref | growth | type | temperature | range | confidence |
---|---|---|---|---|---|
43020 | positive | growth | 65 | thermophilic | |
69480 | thermophilic | 100 |
Physiology and metabolism
oxygen tolerance
- @ref: 69480
- oxygen tolerance: anaerobe
- confidence: 99.561
spore formation
@ref | spore formation | confidence |
---|---|---|
69481 | no | 99 |
69480 | no | 99.809 |
Isolation, sampling and environmental information
isolation
- @ref: 43020
- sample type: deep-sea actively venting hydrothermal sulfidic chimney
- geographic location: East Lau spreading center, Tui Malila hydrothermal field, 1870 m depth
- country: Tonga
- origin.country: TON
- continent: Australia and Oceania
- latitude: -21.9892
- longitude: -176.568
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Condition | #Thermophilic (>45°C) | |
#Environmental | #Aquatic | #Hydrothermal vent |
#Environmental | #Aquatic | #Marine |
Safety information
risk assessment
- @ref: 43020
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 43020
- description: Thermosulfuriphilus ammonigenes 16S ribosomal RNA gene, partial sequence
- accession: KY471008
- length: 1533
- database: ena
- NCBI tax ID: 1936021
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Thermosulfuriphilus ammonigenes ST65 | GCA_011207455 | complete | ncbi | 1936021 |
66792 | Thermosulfuriphilus ammonigenes DSM 102941 | GCA_013761125 | contig | ncbi | 1936021 |
66792 | Thermosulfuriphilus ammonigenes strain DSM 102941 | 1936021.7 | wgs | patric | 1936021 |
66792 | Thermosulfuriphilus ammonigenes DSM 102941 | 2861160354 | draft | img | 1936021 |
GC content
- @ref: 43020
- GC-content: 49.6
- method: thermal denaturation, midpoint method (Tm)
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 99 | no |
motile | yes | 91.997 | no |
flagellated | no | 88.895 | no |
gram-positive | no | 99.66 | no |
anaerobic | yes | 99.593 | no |
aerobic | no | 99.518 | no |
halophile | no | 74.386 | no |
spore-forming | no | 94.603 | no |
thermophile | yes | 99.696 | no |
glucose-util | no | 60.096 | no |
glucose-ferment | no | 85.198 | no |
External links
@ref: 43020
culture collection no.: DSM 102941, VKM B-2855
straininfo link
- @ref: 96689
- straininfo: 400546
literature
- topic: Phylogeny
- Pubmed-ID: 28857038
- title: Thermosulfuriphilus ammonigenes gen. nov., sp. nov., a thermophilic, chemolithoautotrophic bacterium capable of respiratory ammonification of nitrate with elemental sulfur.
- authors: Slobodkina GB, Reysenbach AL, Kolganova TV, Novikov AA, Bonch-Osmolovskaya EA, Slobodkin AI
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijsem.0.002142
- year: 2017
- mesh: Bacteria, Anaerobic/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Hydrothermal Vents/*microbiology, Nitrates/*metabolism, Pacific Ocean, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/microbiology, Sequence Analysis, DNA, Sulfur/*metabolism
- topic2: Metabolism
Reference
@id | authors | title | doi/url | catalogue |
---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
43020 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-102941 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 102941) | |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | Predictions based on the model GenomeNet Sporulation v. 1 | 10.21203/rs.3.rs-2527258/v1 | |
96689 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID400546.1 |