Strain identifier

BacDive ID: 140413

Type strain: Yes

Species: Thermosulfuriphilus ammonigenes

Strain Designation: ST65

Strain history: <- G. Slobodkina, Winogradsky Inst. Microbiol., RAS, Moscow, Russia; ST65 <- G. Slobodkina {2013}

NCBI tax ID(s): 1936021 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 43020

BacDive-ID: 140413

DSM-Number: 102941

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, thermophilic, motile

description: Thermosulfuriphilus ammonigenes ST65 is an anaerobe, thermophilic, motile bacterium that was isolated from deep-sea actively venting hydrothermal sulfidic chimney.

NCBI tax id

  • NCBI tax id: 1936021
  • Matching level: species

strain history

  • @ref: 43020
  • history: <- G. Slobodkina, Winogradsky Inst. Microbiol., RAS, Moscow, Russia; ST65 <- G. Slobodkina {2013}

doi: 10.13145/bacdive140413.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/thermodesulfobacteriota
  • domain: Bacteria
  • phylum: Thermodesulfobacteriota
  • class: Thermodesulfobacteriota, not assigned to class
  • order: Thermodesulfobacteriota, not assigned to order
  • family: Thermodesulfobacteriota, not assigned to family
  • genus: Thermosulfuriphilus
  • species: Thermosulfuriphilus ammonigenes
  • full scientific name: Thermosulfuriphilus ammonigenes Slobodkina et al. 2017

@ref: 43020

domain: Bacteria

phylum: Thermodesulfobacteria

class: Thermodesulfobacteria

order: Thermodesulfobacteria, not assigned to order

family: Thermodesulfobacteriaceae

genus: Thermosulfuriphilus

species: Thermosulfuriphilus ammonigenes

full scientific name: Thermosulfuriphilus ammonigenes Slobodkina et al. 2017

strain designation: ST65

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram stain
69480yes90.947
6948099.979negative

Culture and growth conditions

culture medium

  • @ref: 43020
  • name: THERMOSULFURIPHILUS MEDIUM (DSMZ Medium 1210c)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/1210c
  • composition: Name: THERMOSULFURIPHILUS MEDIUM (DSMZ Medium 1210c) Composition: NaCl 17.9462 g/l Sulfur 4.98504 g/l MgCl2 x 6 H2O 3.98804 g/l Na2S2O3 x 5 H2O 3.48953 g/l Na2CO3 0.997009 g/l KNO3 0.997009 g/l (NH4)2SO4 0.498504 g/l CaCl2 x 2 H2O 0.329013 g/l KCl 0.329013 g/l KH2PO4 0.329013 g/l HCl 0.00249252 g/l FeCl2 x 4 H2O 0.00149551 g/l NaOH 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l Pyridoxine hydrochloride 9.97009e-05 g/l MnCl2 x 4 H2O 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l p-Aminobenzoic acid 4.98504e-05 g/l (DL)-alpha-Lipoic acid 4.98504e-05 g/l Calcium D-(+)-pantothenate 4.98504e-05 g/l Nicotinic acid 4.98504e-05 g/l Riboflavin 4.98504e-05 g/l Thiamine HCl 4.98504e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l Folic acid 1.99402e-05 g/l Biotin 1.99402e-05 g/l H3BO3 5.98205e-06 g/l Na2WO4 x 2 H2O 3.98804e-06 g/l Na2SeO3 x 5 H2O 2.99103e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Vitamin B12 9.97009e-07 g/l Distilled water

culture temp

@refgrowthtypetemperaturerangeconfidence
43020positivegrowth65thermophilic
69480thermophilic100

Physiology and metabolism

oxygen tolerance

  • @ref: 69480
  • oxygen tolerance: anaerobe
  • confidence: 99.561

spore formation

@refspore formationconfidence
69481no99
69480no99.809

Isolation, sampling and environmental information

isolation

  • @ref: 43020
  • sample type: deep-sea actively venting hydrothermal sulfidic chimney
  • geographic location: East Lau spreading center, Tui Malila hydrothermal field, 1870 m depth
  • country: Tonga
  • origin.country: TON
  • continent: Australia and Oceania
  • latitude: -21.9892
  • longitude: -176.568

isolation source categories

Cat1Cat2Cat3
#Condition#Thermophilic (>45°C)
#Environmental#Aquatic#Hydrothermal vent
#Environmental#Aquatic#Marine

Safety information

risk assessment

  • @ref: 43020
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 43020
  • description: Thermosulfuriphilus ammonigenes 16S ribosomal RNA gene, partial sequence
  • accession: KY471008
  • length: 1533
  • database: ena
  • NCBI tax ID: 1936021

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Thermosulfuriphilus ammonigenes ST65GCA_011207455completencbi1936021
66792Thermosulfuriphilus ammonigenes DSM 102941GCA_013761125contigncbi1936021
66792Thermosulfuriphilus ammonigenes strain DSM 1029411936021.7wgspatric1936021
66792Thermosulfuriphilus ammonigenes DSM 1029412861160354draftimg1936021

GC content

  • @ref: 43020
  • GC-content: 49.6
  • method: thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno99no
motileyes91.997no
flagellatedno88.895no
gram-positiveno99.66no
anaerobicyes99.593no
aerobicno99.518no
halophileno74.386no
spore-formingno94.603no
thermophileyes99.696no
glucose-utilno60.096no
glucose-fermentno85.198no

External links

@ref: 43020

culture collection no.: DSM 102941, VKM B-2855

straininfo link

  • @ref: 96689
  • straininfo: 400546

literature

  • topic: Phylogeny
  • Pubmed-ID: 28857038
  • title: Thermosulfuriphilus ammonigenes gen. nov., sp. nov., a thermophilic, chemolithoautotrophic bacterium capable of respiratory ammonification of nitrate with elemental sulfur.
  • authors: Slobodkina GB, Reysenbach AL, Kolganova TV, Novikov AA, Bonch-Osmolovskaya EA, Slobodkin AI
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002142
  • year: 2017
  • mesh: Bacteria, Anaerobic/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Hydrothermal Vents/*microbiology, Nitrates/*metabolism, Pacific Ocean, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/microbiology, Sequence Analysis, DNA, Sulfur/*metabolism
  • topic2: Metabolism

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
43020Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-102941Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 102941)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
96689Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID400546.1