Strain identifier

BacDive ID: 140403

Type strain: Yes

Species: Levilactobacillus cerevisiae

Strain Designation: 2301

Strain history: CIP <- 2017, M. Hutzler, München Univ., Freising, Germany: strain TUM BP 140423000-2250

NCBI tax ID(s): 1704076 (species)

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General

@ref: 43010

BacDive-ID: 140403

DSM-Number: 100836

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, Gram-positive, rod-shaped, colony-forming

description: Levilactobacillus cerevisiae 2301 is a facultative anaerobe, Gram-positive, rod-shaped bacterium that forms circular colonies and was isolated from bright beer tank.

NCBI tax id

  • NCBI tax id: 1704076
  • Matching level: species

strain history

@refhistory
43010<- J. Koob, Research Center Weihenstephan for Brewing and Food Quality, Microbiology, Freising, Germany
118212CIP <- 2017, M. Hutzler, München Univ., Freising, Germany: strain TUM BP 140423000-2250

doi: 10.13145/bacdive140403.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Levilactobacillus
  • species: Levilactobacillus cerevisiae
  • full scientific name: Levilactobacillus cerevisiae (Koob et al. 2017) Zheng et al. 2020

  • synonyms

    • @ref: 20215
    • synonym: Lactobacillus cerevisiae

@ref: 43010

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Levilactobacillus

species: Levilactobacillus cerevisiae

full scientific name: Levilactobacillus cerevisiae (Koob et al. 2017) Zheng et al. 2020

strain designation: 2301

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotilityconfidence
43493positiverod-shapedno
69480no96.419
69480positive100
118212positiverod-shaped

colony morphology

  • @ref: 43493
  • colony color: Beige
  • colony shape: circular
  • incubation period: 2 days
  • medium used: MRS agar

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
43493MRS agaryes
43010MRS MEDIUM (DSMZ Medium 11)yeshttps://mediadive.dsmz.de/medium/11Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
118212CIP Medium 40yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40
118212CIP Medium 41yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=41

culture temp

@refgrowthtypetemperaturerange
43493positivegrowth4-37
43493nogrowth40thermophilic
43493nogrowth45thermophilic
43010positivegrowth30mesophilic

culture pH

  • @ref: 43493
  • ability: positive
  • type: growth
  • pH: 4-7.8
  • PH range: acidophile

Physiology and metabolism

oxygen tolerance

  • @ref: 43493
  • oxygen tolerance: facultative anaerobe

spore formation

@refspore formationconfidence
43493no
69481no100
69480no99.995

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4349327613amygdalin-builds acid from
4349318305arbutin-builds acid from
4349317057cellobiose-builds acid from
4349315963ribitol-builds acid from
4349317108D-arabinose-builds acid from
4349318333D-arabitol-builds acid from
4349328847D-fucose-builds acid from
4349362318D-lyxose-builds acid from
4349316024D-mannose-builds acid from
4349317924D-sorbitol-builds acid from
4349316443D-tagatose-builds acid from
4349316813galactitol-builds acid from
4349317113erythritol-builds acid from
43493esculin ferric citrate-builds acid from
4349328066gentiobiose-builds acid from
4349315978glycerol 3-phosphate-builds acid from
4349328087glycogen-builds acid from
4349315443inulin-builds acid from
4349318403L-arabitol-builds acid from
4349318287L-fucose-builds acid from
4349362345L-rhamnose-builds acid from
4349317266L-sorbose-builds acid from
4349365328L-xylose-builds acid from
4349317716lactose-builds acid from
434936731melezitose-builds acid from
4349343943methyl alpha-D-mannoside-builds acid from
4349374863methyl beta-D-xylopyranoside-builds acid from
4349317268myo-inositol-builds acid from
43493potassium 2-dehydro-D-gluconate-builds acid from
43493potassium 5-dehydro-D-gluconate-builds acid from
4349316634raffinose-builds acid from
4349317814salicin-builds acid from
4349328017starch-builds acid from
4349317992sucrose-builds acid from
4349327082trehalose-builds acid from
4349332528turanose-builds acid from
4349317151xylitol-builds acid from
4349315824D-fructose+builds acid from
4349312936D-galactose+builds acid from
4349317634D-glucose+builds acid from
4349365327D-xylose+builds acid from
4349317306maltose+builds acid from
43493506227N-acetylglucosamine+builds acid from
4349332032potassium gluconate+builds acid from
4349316899D-mannitol+/-builds acid from
4349316988D-ribose+/-builds acid from
4349330849L-arabinose+/-builds acid from
4349328053melibiose+/-builds acid from
43493320061methyl alpha-D-glucopyranoside+/-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose-builds acid from
6837117992sucrose-builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117306maltose+builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
683714853esculin-builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine+builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose-builds acid from
6837115824D-fructose-builds acid from
6837117634D-glucose+builds acid from
6837112936D-galactose+builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose+builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from

metabolite production

  • @ref: 43493
  • Chebi-ID: 16134
  • metabolite: ammonia
  • production: no

enzymes

@refvalueactivityec
43493catalase-1.11.1.6
43493cytochrome oxidase-1.9.3.1

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
43010----+/-+/-+---++----------+-----+------------------+/--+/-

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentgeographic locationisolation date
43010bright beer tankGermanyDEUEurope
43493a contaminated bright beer tank sample from a German breweryGermanyDEUEuropeGermany
118212Food, Spoiled beer sampleGermanyDEUEuropeRheinlandpfalz2014-04-01

isolation source categories

Cat1Cat2Cat3
#Engineered#Built environment#Container (Reservoir)
#Engineered#Contamination
#Engineered#Food production#Fermented
#Engineered#Food production#Beverage

taxonmaps

  • @ref: 69479
  • File name: preview.99_3900.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_45;96_484;97_553;98_1175;99_3900&stattab=map
  • Last taxonomy: Levilactobacillus
  • 16S sequence: KT445896
  • Sequence Identity:
  • Total samples: 1243
  • soil counts: 164
  • aquatic counts: 140
  • animal counts: 727
  • plant counts: 212

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
430101Risk group (German classification)
1182121Risk group (French classification)

Sequence information

16S sequences

  • @ref: 43010
  • description: Lactobacillus cerevisiae 16S ribosomal RNA gene, partial sequence
  • accession: KT445896
  • length: 1477
  • database: ena
  • NCBI tax ID: 1704076

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Levilactobacillus cerevisiae DSM 100836GCA_003946245contigncbi1704076
66792Lactobacillus cerevisiae strain DSM 1008361704076.3wgspatric1704076
66792Levilactobacillus cerevisiae DSM 1008362881695158draftimg1704076

GC content

@refGC-content
4349350
4301050.0

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno93.461no
gram-positiveyes96.017yes
anaerobicno89.656yes
aerobicno94.091yes
halophileyes86.176no
spore-formingno89.937yes
thermophileno99.715no
glucose-utilyes91.73no
flagellatedno98.274yes
glucose-fermentyes83.003no

External links

@ref: 43010

culture collection no.: DSM 100836, TUM BP 140423000-2250, CIP 111489, LMG 29073

straininfo link

  • @ref: 96682
  • straininfo: 408134

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny28867002Lactobacillus cerevisiae sp. nov., isolated from a spoiled brewery sample.Koob J, Jacob F, Wenning M, Hutzler MInt J Syst Evol Microbiol10.1099/ijsem.0.0021392017Bacterial Typing Techniques, Base Composition, Beer/*microbiology, DNA, Bacterial/genetics, Fatty Acids/chemistry, Genes, Bacterial, Germany, Lactobacillus/*classification/genetics/isolation & purification, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNATranscriptome
Phylogeny32129738Lactobacillus enshiensis sp. nov., a novel arsenic-resistant bacterium.Zhang Z, Wang Y, Hou Q, Zhao H, Li W, Sun Z, Guo ZInt J Syst Evol Microbiol10.1099/ijsem.0.0040722020*Arsenic, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Fermentation, Fermented Foods/*microbiology, *Food Microbiology, Lactic Acid/biosynthesis, Lactobacillus/*classification/isolation & purification, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNABiotechnology

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
43010Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-100836Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 100836)
43493Jennifer Koob, Fritz Jacob, Mareike Wenning, Mathias HutzlerLactobacillus cerevisiae sp. nov., isolated from a spoiled brewery sample10.1099/ijsem.0.002139IJSEM 67: 3603-3609 201728867002
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68371Automatically annotated from API 50CH acid
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
96682Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID408134.1
118212Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20111489Collection of Institut Pasteur (CIP 111489)