Strain identifier

BacDive ID: 14039

Type strain: Yes

Species: Sanguibacter suarezii

Strain Designation: ST-26

Strain history: CIP <- 1999, CECT <- J.F. Fernandez-Garayzabal: strain ST-26

NCBI tax ID(s): 1223525 (strain), 60921 (species)

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General

@ref: 3919

BacDive-ID: 14039

DSM-Number: 10543

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive, motile, rod-shaped

description: Sanguibacter suarezii ST-26 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from bovine blood.

NCBI tax id

NCBI tax idMatching level
60921species
1223525strain

strain history

@refhistory
3919<- NCFB <- J.F. Fernández-Garayzábal, ST-26
67770IFO 16159 <-- NCIMB 703023 <-- J. F. Fernández-Garayzábal ST-26.
123341CIP <- 1999, CECT <- J.F. Fernandez-Garayzabal: strain ST-26

doi: 10.13145/bacdive14039.20250331.9.3

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Jonesiaceae
  • genus: Sanguibacter
  • species: Sanguibacter suarezii
  • full scientific name: Sanguibacter suarezii Fernández-Garayzábal et al. 1995

@ref: 3919

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Jonesiaceae

genus: Sanguibacter

species: Sanguibacter suarezii

full scientific name: Sanguibacter suarezii Fernández-Garayzábal et al. 1995 emend. Pikuta et al. 2017

strain designation: ST-26

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotilityconfidence
123341positiverod-shapedyes
125438positive91.468
125439positive99.8

colony morphology

@refcolony colorincubation periodmedium used
18355Zinc yellow (1018)10-14 daysISP 3
1835510-14 daysISP 4
1835510-14 daysISP 5
1835510-14 daysISP 6
1835510-14 daysISP 7
123341

multicellular morphology

@refforms multicellular complexmedium name
18355noISP 3
18355noISP 4
18355noISP 5

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
3919TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
18355ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
18355ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
18355ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18355ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
18355ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
32955MEDIUM 338 - for Sanguibacter keddieiiyesDistilled water make up to (1000.000 ml);Agar (15.000 g);Yeast extract (3.000 g);Trypto casein soy agar (30.000 g)
123341CIP Medium 338yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=338

culture temp

@refgrowthtypetemperature
3919positivegrowth30
18355positiveoptimum28
32955positivegrowth30
53329positivegrowth30
67770positivegrowth28
123341positivegrowth10-30
123341nogrowth37
123341nogrowth41
123341nogrowth45

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
53329aerobe
123341facultative anaerobe
125439obligate aerobe94.5

halophily

@refsaltgrowthtested relationconcentration
18355NaClpositivemaximum2.5 %
123341NaClpositivegrowth0-10 %

murein

  • @ref: 3919
  • murein short key: A11.48
  • type: A4alpha L-Lys-L-Ser-D-Glu

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1835517234glucose-
1835522599arabinose+
1835517992sucrose+
1835518222xylose-
1835517268myo-inositol+
1835529864mannitol+
1835528757fructose+
1835526546rhamnose+
1835516634raffinose+
1835562968cellulose-
6837928087glycogen+fermentation
6837917306maltose+fermentation
6837965327D-xylose+fermentation
6837916988D-ribose+fermentation
6837917634D-glucose+fermentation
6837916199urea-hydrolysis
683794853esculin+hydrolysis
6837917632nitrate+reduction
12334116947citrate-carbon source
1233414853esculin+hydrolysis
123341606565hippurate-hydrolysis
12334117632nitrate+reduction
12334116301nitrite-reduction
12334117632nitrate+respiration
68371Potassium 5-ketogluconate-builds acid from
6837118305arbutin+builds acid from
6837127613amygdalin+builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837917992sucrose+fermentation
6837917716lactose+fermentation
6837916899D-mannitol+fermentation
683795291gelatin-hydrolysis
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose+builds acid from
6837132528turanose+builds acid from
6837128066gentiobiose+builds acid from
6837117151xylitol-builds acid from
6837128087glycogen+builds acid from
6837128017starch+builds acid from
6837116634raffinose+builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose+builds acid from
6837117992sucrose+builds acid from
6837128053melibiose+builds acid from
6837117716lactose+builds acid from
6837117306maltose+builds acid from
6837117057cellobiose+builds acid from
6837117814salicin+builds acid from
683714853esculin+builds acid from
6837159640N-acetylglucosamine-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose+builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose+builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose+builds acid from
6837112936D-galactose+builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose+builds acid from
6837116988D-ribose+builds acid from
6837130849L-arabinose+builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol+builds acid from
683685291gelatin-hydrolysis
6836827897tryptophan-energy source
6836816199urea-hydrolysis
6836816947citrate-assimilation
6836818257ornithine-degradation
6836825094lysine-degradation
6836829016arginine-hydrolysis

antibiotic resistance

  • @ref: 123341
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

@refChebi-IDmetaboliteproduction
12334135581indoleno
6836815688acetoinno
6836835581indoleno
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole testmethylred-test
6836815688acetoin-
6836835581indole-
12334115688acetoin-
12334117234glucose+

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-galactosidase+3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
123341oxidase-
123341beta-galactosidase+3.2.1.23
123341alcohol dehydrogenase-1.1.1.1
123341gelatinase-
123341amylase+
123341DNase+
123341caseinase-3.4.21.50
123341catalase+1.11.1.6
123341tween esterase-
123341gamma-glutamyltransferase-2.3.2.2
123341lecithinase-
123341lipase-
123341lysine decarboxylase-4.1.1.18
123341ornithine decarboxylase-4.1.1.17
123341phenylalanine ammonia-lyase-4.3.1.24
123341protease-
123341tryptophan deaminase-
123341urease-3.5.1.5
68379N-acetyl-beta-glucosaminidase+3.2.1.52
68379beta-galactosidase+3.2.1.23
68379beta-glucuronidase-3.2.1.31
68368ornithine decarboxylase-4.1.1.17
68382cystine arylamidase-3.4.11.3
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
68379gelatinase-
68379urease-3.5.1.5
68379beta-glucosidase+3.2.1.21
68379alpha-glucosidase+3.2.1.20
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase-3.5.1.B15
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase-3.2.1.23

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
18355+--+-++++--++++++++

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- FucosidaseControl
18355+++-++---++++-+++--
123341+++-+----++++-+++---

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
18355-----------

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
123341+--+++---++++-+-------++++++++++--+++-+++--------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
123341---------------------------------------------------------------------------------------------------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
3919bovine bloodSpainESPEurope
53329Bovine blood
67770Venous blood from healthy cow
123341Animal, Bovine, bloodSpainESPEurope

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Bovinae (Cow, Cattle)
#Host Body Product#Fluids#Blood

taxonmaps

  • @ref: 69479
  • File name: preview.99_6923.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_9;96_3136;97_3840;98_4877;99_6923&stattab=map
  • Last taxonomy: Sanguibacter suarezii
  • 16S sequence: X79452
  • Sequence Identity:
  • Total samples: 1745
  • soil counts: 392
  • aquatic counts: 350
  • animal counts: 770
  • plant counts: 233

Safety information

risk assessment

@refpathogenicity animalbiosafety levelbiosafety level comment
3919yes, in single cases1Risk group (German classification)
183552German classification
1233411Risk group (French classification)

Sequence information

16S sequences

  • @ref: 3919
  • description: Sanguibacter inulinus ST26 16S rDNA
  • accession: X79452
  • length: 1491
  • database: nuccore
  • NCBI tax ID: 60921

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Sanguibacter suarezii NBRC 161591223525.3wgspatric1223525
66792Sanguibacter suarezii NBRC 161592731957599draftimg1223525
67770Sanguibacter suarezii NBRC 16159GCA_001552735contigncbi1223525

GC content

  • @ref: 3919
  • GC-content: 69.5
  • method: thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

@refmodeltraitdescriptionpredictionconfidencetraining_data
125439BacteriaNetoxygen_toleranceOxygenic conditions needed for growthobligate aerobe94.5
125439BacteriaNetgram_stainReaction to gram-stainingpositive99.8
125439BacteriaNetmotilityAbility to perform movementno86.2
125439BacteriaNetspore_formationAbility to form endo- or exosporesyes59.3
125438gram-positivegram-positivePositive reaction to Gram-stainingyes91.468no
125438anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no92.101no
125438spore-formingspore-formingAbility to form endo- or exosporesno57.875no
125438aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)yes66.942no
125438thermophilethermophilicAbility to grow at temperatures above or equal to 45°Cno99.467yes
125438motile2+flagellatedAbility to perform flagellated movementno61.5no

External links

@ref: 3919

culture collection no.: DSM 10543, NCFB 3023, CCUG 36691, JCM 11430, ATCC 51766, CECT 4539, CGMCC 1.3609, CIP 106322, IFO 16159, NBRC 16159, NCIMB 703023

straininfo link

@refstraininfo
83207125865
83208312113

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny18319451Sanguibacter soli sp. nov., isolated from soil of a ginseng field.Kim MK, Pulla RK, Kim SY, Yi TH, Soung NK, Yang DCInt J Syst Evol Microbiol10.1099/ijs.0.65399-02008Actinomycetales/classification/genetics/*isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Fatty Acids/analysis, Genes, rRNA, Korea, Molecular Sequence Data, Panax/*growth & development, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Species SpecificityGenetics
Phylogeny28150571Sanguibacter gelidistatuariae sp. nov., a novel psychrotolerant anaerobe from an ice sculpture in Antarctica, and emendation of descriptions of the family Sanguibacteraceae, the genus Sanguibacter and species S. antarcticus, S. inulinus, S. kedieii, S. marinus, S. soli and S. suarezii.Pikuta EV, Lyu Z, Williams MD, Patel NB, Liu Y, Hoover RB, Busse HJ, Lawson PA, Whitman WBInt J Syst Evol Microbiol10.1099/ijsem.0.0018382017Actinomycetales/*classification/genetics/isolation & purification, Antarctic Regions, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, *Ice, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sculpture, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistryTranscriptome
Phylogeny34100698Flavimobilis rhizosphaerae sp. nov., isolated from rhizosphere soil of Spartina alterniflora.Cai H, Shi S, Wu J, Yang L, Wang F, Jiang C, Gao C, Yang S, Jiang M, Jiang YInt J Syst Evol Microbiol10.1099/ijsem.0.0048292021Actinobacteria/*classification/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, Poaceae/*microbiology, RNA, Ribosomal, 16S/genetics, *Rhizosphere, Sequence Analysis, DNA, *Soil MicrobiologyTranscriptome

Reference

@idauthorscataloguedoi/urltitle
3919Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 10543)https://www.dsmz.de/collection/catalogue/details/culture/DSM-10543
18355Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM10543.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
32955Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/18440
53329Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 36691)https://www.ccug.se/strain?id=36691
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68371Automatically annotated from API 50CH acid
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
83207Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID125865.1StrainInfo: A central database for resolving microbial strain identifiers
83208Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID312113.1StrainInfo: A central database for resolving microbial strain identifiers
123341Curators of the CIPCollection of Institut Pasteur (CIP 106322)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106322
125438Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann10.1101/2024.08.12.607695Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets
125439Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardyhttps://github.com/GenomeNet/deepGdeepG: Deep Learning for Genome Sequence Data. R package version 0.3.1