Strain identifier

BacDive ID: 140292

Type strain: Yes

Species: Flaviaestuariibacter luteus

Strain Designation: AW305

Strain history: <- A. Geppert, Leibniz-Institut DSMZ GmbH, Braunschweig, Germany; AW305 <- B. Fösel and A. Geppert

NCBI tax ID(s): 1789691 (species)

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General

@ref: 42899

BacDive-ID: 140292

DSM-Number: 100282

keywords: 16S sequence, Bacteria, aerobe, chemoorganoheterotroph, Gram-negative, rod-shaped, colony-forming

description: Flaviaestuariibacter luteus AW305 is an aerobe, chemoorganoheterotroph, Gram-negative bacterium that forms circular colonies and was isolated from semi-arid agricultural floodplain soil used for maize cultivation.

NCBI tax id

  • NCBI tax id: 1789691
  • Matching level: species

strain history

  • @ref: 42899
  • history: <- A. Geppert, Leibniz-Institut DSMZ GmbH, Braunschweig, Germany; AW305 <- B. Fösel and A. Geppert

doi: 10.13145/bacdive140292.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Chitinophagia
  • order: Chitinophagales
  • family: Chitinophagaceae
  • genus: Flaviaestuariibacter
  • species: Flaviaestuariibacter luteus
  • full scientific name: Flaviaestuariibacter luteus corrig. Pascual et al. 2017

  • synonyms

    • @ref: 20215
    • synonym: Flaviaesturariibacter luteus

@ref: 42899

domain: Bacteria

phylum: Bacteroidetes

class: Chitinophagia

order: Sphingobacteriales

family: Chitinophagaceae

genus: Flaviaesturariibacter

species: Flaviaesturariibacter luteus

full scientific name: Flaviaesturariibacter luteus Pascual et al. 2017

strain designation: AW305

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
43231negative0.7-3.5 µm0.7-1.0 µmrod-shapedno
43231coccus-shaped

colony morphology

  • @ref: 43231
  • colony size: 1 mm
  • colony color: yellow
  • colony shape: circular
  • incubation period: 7 days
  • medium used: solid SSE/HD 1:10 medium

pigmentation

  • @ref: 43231
  • production: no
  • name: flexirubin-type pigments

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
43231solid SSE/HD 1:10 mediumyes
42899SSE/HD1:10 (DSMZ Medium 1426)yeshttps://mediadive.dsmz.de/medium/1426Name: SSE/HD 1:10 MEDIUM (DSMZ Medium 1426) Composition: MES 1.95 g/l Peptone 0.5 g/l CaSO4 x 2 H2O 0.4303 g/l MgSO4 x 7 H2O 0.3695 g/l Yeast extract 0.25 g/l Na NO3 0.212 g/l CaCl2 x 2 H2O 0.1469 g/l Ca(NO3)2 x 4 H2O 0.118 g/l MgCl2 x 6 H2O 0.1018 g/l Glucose 0.1 g/l (NH4)2SO4 0.09915 g/l NH4Cl 0.05345 g/l K2SO4 0.0435 g/l FeSO4 x 7 H2O 0.00555 g/l KH2PO4 0.00340213 g/l HCl 0.0025 g/l FeCl2 x 4 H2O 0.0015 g/l CoCl2 x 6 H2O 0.00019 g/l MnCl2 x 4 H2O 0.0001 g/l ZnCl2 7e-05 g/l Na2MoO4 x 2 H2O 3.6e-05 g/l NiCl2 x 6 H2O 2.4e-05 g/l Pyridoxine hydrochloride 1e-05 g/l H3BO3 6e-06 g/l Lipoic acid 5e-06 g/l D-Calcium pantothenate 5e-06 g/l Nicotinic acid 5e-06 g/l Riboflavin 5e-06 g/l Thiamine-HCl x 2 H2O 5e-06 g/l p-Aminobenzoic acid 5e-06 g/l Folic acid 2e-06 g/l Biotin 2e-06 g/l CuCl2 x 2 H2O 2e-06 g/l Vitamin B12 1e-07 g/l Distilled water
42899R2A MEDIUM (DSMZ Medium 830)yeshttps://mediadive.dsmz.de/medium/830Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
43231positiveoptimum33-40
43231positivegrowth15-40
42899positivegrowth28mesophilic

culture pH

@refabilitytypepH
43231positivegrowth5.3-8.3
43231positiveoptimum5.8-7.9

Physiology and metabolism

oxygen tolerance

  • @ref: 43231
  • oxygen tolerance: aerobe

nutrition type

  • @ref: 43231
  • type: chemoorganoheterotroph

spore formation

  • @ref: 43231
  • type of spore: spore
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
43231NaClpositivemaximum0.25 %(w/v)
43231NaClpositiveoptimum0 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4323117632nitrate-reduction
4323117234glucose-fermentation
432315291gelatin+hydrolysis
43231yeast extract+growth
43231peptone+growth
43231casamino acids+growth
4323130089acetate+growth
4323115740formate+growth
4323117968butyrate+growth
43231370543-hydroxybutyrate+growth
43231167244-hydroxybutyrate+growth
4323118186tyrosine+growth
4323128044phenylalanine+growth
4323116467L-arginine+growth
4323136241protocatechuate+growth
432316364laminarin+growth
4323130849L-arabinose-growth
4323117057cellobiose-growth
4323115824D-fructose-growth
4323112936D-galactose-growth
4323117634D-glucose-growth
4323117716lactose-growth
4323162318D-lyxose-growth
4323117306maltose-growth
4323116024D-mannose-growth
432316731melezitose-growth
4323116634raffinose-growth
4323162345L-rhamnose-growth
4323117992sucrose-growth
4323127082trehalose-growth
4323118222xylose-growth
4323133946erythrose-growth
4323123958erythrulose-growth
4323118287L-fucose-growth
4323117266L-sorbose-growth
432315417glucosamine-growth
4323128037N-acetylgalactosamine-growth
43231506227N-acetylglucosamine-growth
4323115688acetoin-growth
4323118333D-arabitol-growth
4323116813galactitol-growth
4323122605arabinitol-growth
4323116899D-mannitol-growth
4323117924D-sorbitol-growth
4323117151xylitol-growth
4323115963ribitol-growth
4323117268myo-inositol-growth
4323135391aspartate-growth
4323116977L-alanine-growth
4323117196L-asparagine-growth
4323117561L-cysteine-growth
4323118050L-glutamine-growth
4323115428glycine-growth
4323127570histidine-growth
4323117191L-isoleucine-growth
4323161995lecithin-growth
4323118019L-lysine-growth
4323116811methionine-growth
4323118257ornithine-growth
4323117822serine-growth
43231L-proline-4-nitroanilide-growth
4323126986threonine-growth
4323127897tryptophan-growth
4323127266valine-growth
4323117128adipate-growth
4323122651ascorbate-growth
4323116150benzoate-growth
43231trimethoxybenzoate-growth
43231645522-hydroxybutyrate-growth
4323148944isobutyrate-growth
4323129985L-glutamate-growth
4323124741hydroxyproline-growth
432318391D-gluconate-growth
4323127689decanoate-growth
4323125646octanoate-growth
4323116947citrate-growth
4323116087isocitrate-growth
4323135899crotonate-growth
4323129806fumarate-growth
4323130031succinate-growth
432312-oxogluconate-growth
4323124297glucuronate-growth
43231309162-oxoglutarate-growth
4323129805glycolate-growth
4323136655glyoxylate-growth
4323132362heptanoate-growth
4323148942isovalerate-growth
43231391504-oxopentanoate-growth
4323124996lactate-growth
4323125115malate-growth
4323118300maleic acid-growth
4323115792malonate-growth
4323132544nicotinate-growth
4323116452oxaloacetate-growth
4323117272propionate-growth
4323115361pyruvate-growth
4323136208shikimate-growth
4323130929tartrate-growth
43231286442-oxopentanoate-growth
43231356862-butanol-growth
43231526821,2-butandiol-growth
43231620642,3-butanediol-growth
4323116236ethanol-growth
4323130742ethylene glycol-growth
4323117790methanol-growth
4323128831propanol-growth
43231169971,2-propandiol-growth
43231fermented rumen extract-growth
4323153426tween 80-growth
4323117754glycerol-growth
43231casein hydrolysate-growth
4323128017starch+hydrolysis
4323162968cellulose+hydrolysis
4323185146carboxymethylcellulose-hydrolysis
4323137166xylan-hydrolysis
4323117029chitin-hydrolysis
4323117309pectin-hydrolysis
432316457lignin-hydrolysis
4323162969polygalacturonic acid-hydrolysis
4323153258sodium citrate-growth

metabolite production

  • @ref: 43231
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
43231cytochrome oxidase-1.9.3.1
43231catalase+1.11.1.6
43231alkaline phosphatase+3.1.3.1
43231acid phosphatase+3.1.3.2
43231naphthol-AS-BI-phosphohydrolase+
43231leucine arylamidase+3.4.11.1
43231valine arylamidase+
43231cystine arylamidase+3.4.11.3
43231trypsin+3.4.21.4
43231alpha-chymotrypsin+3.4.21.1
43231alpha-glucosidase+3.2.1.20
43231beta-glucosidase+3.2.1.21
43231N-acetyl-beta-glucosaminidase+3.2.1.52
43231urease-3.5.1.5
43231arginine dihydrolase-3.5.3.6
43231alpha-galactosidase-3.2.1.22
43231alpha-fucosidase-3.2.1.51
43231beta-glucuronidase-3.2.1.31
43231alpha-mannosidase-3.2.1.24
43231esterase (C 4)-
43231esterase Lipase (C 8)-
43231lipase (C 14)-
43231beta-galactosidase+/-3.2.1.23

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    43231C15:02
    43231C16:05.3
    43231C15:0 iso19.5
    43231C17:0 iso1.2
    43231C15:1 iso G27.7
    43231C16:1ω5c14.2
    43231C16:0 3OH1.4
    43231C11:0 iso 3OH2.6
    43231C17:0 iso 3OH11.3
    43231C16:1ω7c / C15:0 iso 2OH10.8
    43231Unknown ECL 16.5801.116580
  • type of FA analysis: whole cell analysis
  • incubation medium: SSE/HD 1:10 medium
  • incubation temperature: 25
  • incubation time: 6
  • system: MIS MIDI
  • cutoff value:

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudesampling date
42899semi-arid agricultural floodplain soil used for maize cultivationKavango region, Mashare (17° 53' 32.4'' S 20° 11' 15.4'' E)NamibiaNAMAfrica-17.892320.1876
43231an agricultural floodplain soilMashare, Kavango region, NamibiaNamibiaNAMAfrica-17202012

isolation source categories

Cat1Cat2Cat3
#Engineered#Agriculture
#Environmental#Terrestrial#Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_165807.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_185;96_26009;97_32634;98_108862;99_165807&stattab=map
  • Last taxonomy: Flaviaesturariibacter luteus subclade
  • 16S sequence: LT158298
  • Sequence Identity:
  • Total samples: 3623
  • soil counts: 2674
  • aquatic counts: 296
  • animal counts: 329
  • plant counts: 324

Safety information

risk assessment

  • @ref: 42899
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 42899
  • description: Flaviaesturariibacter sp. DSM 100282 partial 16S rRNA gene, type strain AW305 = DSM 100282T
  • accession: LT158298
  • length: 1493
  • database: ena
  • NCBI tax ID: 1789691

GC content

  • @ref: 42899
  • GC-content: 57.6
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 42899

culture collection no.: DSM 100282, LMG 29416

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny28632115Flaviaesturariibacter luteus sp. nov., isolated from an agricultural floodplain soil, and emended description of the genus Flaviaesturariibacter.Pascual J, Foesel BU, Geppert A, Huber KJ, Overmann JInt J Syst Evol Microbiol10.1099/ijsem.0.0018542017Agriculture, Bacterial Typing Techniques, Bacteroidetes/*classification/genetics/isolation & purification, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Namibia, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistryTranscriptome
Phylogeny33392675Flaviaesturariibacter aridisoli sp. nov., A Bacterium Isolated from Dry Soil.Lee SE, Han JH, Sathiyaraj G, Srinivasan SCurr Microbiol10.1007/s00284-020-02312-92021Bacterial Typing Techniques, Bacteroidetes, DNA, Bacterial/genetics, Fatty Acids/analysis, Islands, Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, *Soil, *Soil MicrobiologyTranscriptome

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
42899Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-100282Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 100282)
43231Javier Pascual, Bärbel U. Foesel, Alicia Geppert, Katharina J. Huber and Jörg OvermannFlaviaesturariibacter luteus sp. nov., isolated from an agricultural floodplain soil, and emended description of the genus Flaviaesturariibacter10.1099/ijsem.0.001854IJSEM 67: 1727-1734 201728632115
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/