Strain identifier

BacDive ID: 140288

Type strain: Yes

Species: Mycobacterium persicum

Strain Designation: AFPC-000227

Strain history: CIP <- 2016, E. Tortoli, San Raffaele Scientific Inst., Milano, Italy: strain AFPC000227

NCBI tax ID(s): 1487726 (species)

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General

@ref: 42895

BacDive-ID: 140288

DSM-Number: 104278

keywords: genome sequence, 16S sequence, Bacteria, thermophilic, Gram-positive, rod-shaped

description: Mycobacterium persicum AFPC-000227 is a thermophilic, Gram-positive, rod-shaped bacterium that was isolated from sputum from a patient with fever, productive cough and shortness of breath.

NCBI tax id

  • NCBI tax id: 1487726
  • Matching level: species

strain history

@refhistory
42895<- E. Tortoli, San Raffaele Scientific Institute, Milan, Italy; AFPC-000227 <- A.H. Shahraki; Pasture Institute of Iran, Dep. of Epidemiology, Teheran; AFPC-000227
121689CIP <- 2016, E. Tortoli, San Raffaele Scientific Inst., Milano, Italy: strain AFPC000227

doi: 10.13145/bacdive140288.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Mycobacteriaceae
  • genus: Mycobacterium
  • species: Mycobacterium persicum
  • full scientific name: Mycobacterium persicum Shahraki et al. 2017

@ref: 42895

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Mycobacteriaceae

genus: Mycobacterium

species: Mycobacterium persicum

full scientific name: Mycobacterium persicum Shahraki et al. 2017

strain designation: AFPC-000227

type strain: yes

Morphology

cell morphology

  • @ref: 121689
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

multimedia

  • @ref: 42895
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_104278.jpg
  • caption: Medium 645 37°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
43241MacConkey agar without crystal violetno
42895MIDDLEBROOK MEDIUM (DSMZ Medium 645)yeshttps://mediadive.dsmz.de/medium/645Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water
121689CIP Medium 55yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=55

culture temp

@refgrowthtypetemperaturerange
43241nogrowth42thermophilic
43241positivegrowth25mesophilic
43241positivegrowth37mesophilic
42895positivegrowth37mesophilic

Physiology and metabolism

observation

@refobservation
43241niacin accumulation
43241Yellow pigmentation developed only after light exposure

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4324117632nitrate+reduction
4324153426tween 80+hydrolysis
4324130477tellurite-reduction

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitive
432412637amikacinyesyes
432413732clarithromycinyesyes
4324163607linezolidyesyes
4324163611moxifloxacinyesyes
4324128077rifampicinyesyes

enzymes

@refvalueactivityec
43241catalase+1.11.1.6
43241urease-3.5.1.5
43241beta-glucosidase-3.2.1.21

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
42895sputum from a patient with fever, productive cough and shortness of breathTeheran General HospitalIranIRNAsia
43241pulmonary specimens of Iranian patient (female) with pulmonary diseaseTehran, IranIranIRNAsia2009
121689Human, Sputum sampleTehranIranIRNAsia2009-01-02

isolation source categories

Cat1Cat2Cat3
#Host Body Product#Fluids#Sputum
#Infection#Patient

taxonmaps

  • @ref: 69479
  • File name: preview.99_75.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_37;97_39;98_43;99_75&stattab=map
  • Last taxonomy: Mycobacterium
  • 16S sequence: KX987140
  • Sequence Identity:
  • Total samples: 1520
  • soil counts: 881
  • aquatic counts: 382
  • animal counts: 203
  • plant counts: 54

Safety information

risk assessment

  • @ref: 121689
  • biosafety level: 1
  • biosafety level comment: Risk group (French classification)

Sequence information

16S sequences

  • @ref: 42895
  • description: Mycobacterium persicum strain AFPC-000227 16S ribosomal RNA gene, partial sequence
  • accession: KX987140
  • length: 1527
  • database: ena
  • NCBI tax ID: 1487726

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Mycobacterium persicum AFPC-000227GCA_002086675contigncbi1487726
66792Mycobacterium sp. AFPC-000227 strain AFPC-0002271487726.3wgspatric1487726

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno95.843no
gram-positiveyes86.386no
anaerobicno98.436no
halophileno93.047no
spore-formingno85.702no
thermophileno98.402no
glucose-utilyes64.473no
flagellatedno98.842no
aerobicyes87.977no
glucose-fermentno90.45no

External links

@ref: 42895

culture collection no.: DSM 104278, CIP 111197, AFPC 000207, AFPC 000227

straininfo link

  • @ref: 96594
  • straininfo: 407602

literature

  • topic: Phylogeny
  • Pubmed-ID: 28629501
  • title: Mycobacterium persicum sp. nov., a novel species closely related to Mycobacterium kansasii and Mycobacterium gastri.
  • authors: Shahraki AH, Trovato A, Mirsaeidi M, Borroni E, Heidarieh P, Hashemzadeh M, Shahbazi N, Cirillo DM, Tortoli E
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001862
  • year: 2017
  • mesh: Bacterial Typing Techniques, DNA, Bacterial/genetics, Female, Genes, Bacterial, Humans, Iran, Lung/*microbiology, Male, Middle Aged, Mycobacterium/*classification/genetics/isolation & purification, Mycobacterium kansasii, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA
  • topic2: Pathogenicity

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
42895Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-104278Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 104278)
43241Abdolrazagh Hashemi Shahraki, Alberto Trovato, Mehdi Mirsaeidi, Emanuele Borroni, Parvin Heidarieh, Mohamad Hashemzadeh, Narges Shahbazi, Daniela M. Cirillo and Enrico TortoliMycobacterium persicum sp. nov., a novel species closely related to Mycobacterium kansasii and Mycobacterium gastri10.1099/ijsem.0.001862IJSEM 67: 1766-1770 201728629501
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
96594Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID407602.1
121689Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20111197Collection of Institut Pasteur (CIP 111197)