Strain identifier
BacDive ID: 140276
Type strain:
Species: Halomonas alkalicola
Strain Designation: 56-L4-10aEn
Strain history: <- Y. Ge, CICC; 56-L4-10aEn
NCBI tax ID(s): 1930622 (species)
General
@ref: 42883
BacDive-ID: 140276
DSM-Number: 103354
keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, rod-shaped, colony-forming
description: Halomonas alkalicola 56-L4-10aEn is an aerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from soap-making tank of a household product plant.
NCBI tax id
- NCBI tax id: 1930622
- Matching level: species
strain history
- @ref: 42883
- history: <- Y. Ge, CICC; 56-L4-10aEn
doi: 10.13145/bacdive140276.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Oceanospirillales
- family: Halomonadaceae
- genus: Halomonas
- species: Halomonas alkalicola
- full scientific name: Halomonas alkalicola Tang et al. 2017
@ref: 42883
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Oceanospirillales
family: Halomonadaceae
genus: Halomonas
species: Halomonas alkalicola
full scientific name: Halomonas alkalicola Tang et al. 2017
strain designation: 56-L4-10aEn
type strain: yes
Morphology
cell morphology
- @ref: 43185
- gram stain: negative
- cell length: 1.5-2.1 µm
- cell width: 0.5-0.6 µm
- cell shape: rod-shaped
- motility: no
colony morphology
- @ref: 43185
- colony color: ochre
- colony shape: circular
- medium used: tryptic soy agar (TSA)
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
43185 | tryptic soy agar (TSA) | yes | ||
42883 | BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) | yes | https://mediadive.dsmz.de/medium/514 | Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
43185 | positive | optimum | 28-30 | mesophilic |
43185 | positive | growth | 15-37 | |
42883 | positive | growth | 28 | mesophilic |
culture pH
@ref | ability | type | pH | PH range |
---|---|---|---|---|
43185 | positive | growth | 7-12.5 | alkaliphile |
43185 | positive | optimum | 9-10 |
Physiology and metabolism
oxygen tolerance
- @ref: 43185
- oxygen tolerance: aerobe
spore formation
- @ref: 43185
- spore formation: no
halophily
@ref | salt | growth | tested relation | concentration | halophily level |
---|---|---|---|---|---|
43185 | NaCl | positive | growth | 0-8 %(w/v) | |
43185 | NaCl | positive | optimum | 0-2 %(w/v) | |
43185 | moderately halophilic |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
43185 | 17754 | glycerol | - | builds acid from |
43185 | 17113 | erythritol | - | builds acid from |
43185 | 17108 | D-arabinose | - | builds acid from |
43185 | 30849 | L-arabinose | - | builds acid from |
43185 | 16988 | D-ribose | - | builds acid from |
43185 | 65327 | D-xylose | - | builds acid from |
43185 | 65328 | L-xylose | - | builds acid from |
43185 | 15963 | ribitol | - | builds acid from |
43185 | 16024 | D-mannose | - | builds acid from |
43185 | 17266 | L-sorbose | - | builds acid from |
43185 | 62345 | L-rhamnose | - | builds acid from |
43185 | 16813 | galactitol | - | builds acid from |
43185 | 17268 | myo-inositol | - | builds acid from |
43185 | 29864 | mannitol | - | builds acid from |
43185 | 30911 | sorbitol | - | builds acid from |
43185 | 24265 | gluconate | - | builds acid from |
43185 | 320061 | methyl alpha-D-glucopyranoside | - | builds acid from |
43185 | 506227 | N-acetylglucosamine | - | builds acid from |
43185 | 27613 | amygdalin | - | builds acid from |
43185 | 18305 | arbutin | - | builds acid from |
43185 | 17814 | salicin | - | builds acid from |
43185 | 17057 | cellobiose | - | builds acid from |
43185 | 17716 | lactose | - | builds acid from |
43185 | 28053 | melibiose | - | builds acid from |
43185 | 15443 | inulin | - | builds acid from |
43185 | 28017 | starch | - | builds acid from |
43185 | 28087 | glycogen | - | builds acid from |
43185 | 17151 | xylitol | - | builds acid from |
43185 | 28066 | gentiobiose | - | builds acid from |
43185 | 62318 | D-lyxose | - | builds acid from |
43185 | 16443 | D-tagatose | - | builds acid from |
43185 | 18287 | L-fucose | - | builds acid from |
43185 | 18333 | D-arabitol | - | builds acid from |
43185 | 18403 | L-arabitol | - | builds acid from |
43185 | 43943 | methyl alpha-D-mannoside | - | builds acid from |
43185 | 16808 | 2-dehydro-D-gluconate | - | builds acid from |
43185 | 58143 | 5-dehydro-D-gluconate | - | builds acid from |
43185 | 12936 | D-galactose | - | builds acid from |
43185 | 17634 | D-glucose | - | builds acid from |
43185 | 15824 | D-fructose | - | builds acid from |
43185 | 17306 | maltose | - | builds acid from |
43185 | 27082 | trehalose | - | builds acid from |
43185 | 17992 | sucrose | - | builds acid from |
43185 | 6731 | melezitose | - | builds acid from |
43185 | 32528 | turanose | - | builds acid from |
43185 | 28847 | D-fucose | - | builds acid from |
43185 | 16634 | raffinose | + | builds acid from |
43185 | 16991 | dna | - | hydrolysis |
43185 | 4853 | esculin | - | hydrolysis |
43185 | 5291 | gelatin | - | hydrolysis |
43185 | 28017 | starch | - | hydrolysis |
43185 | 53424 | tween 20 | - | hydrolysis |
43185 | 53426 | tween 80 | - | hydrolysis |
43185 | 18186 | tyrosine | - | hydrolysis |
43185 | 16947 | citrate | - | carbon source |
43185 | 17632 | nitrate | - | reduction |
43185 | 17234 | glucose | - | assimilation |
43185 | 22599 | arabinose | - | assimilation |
43185 | 37684 | mannose | - | assimilation |
43185 | 29864 | mannitol | - | assimilation |
43185 | 506227 | N-acetylglucosamine | - | assimilation |
43185 | 17306 | maltose | - | assimilation |
43185 | 24265 | gluconate | - | assimilation |
43185 | 27689 | decanoate | - | assimilation |
43185 | 17128 | adipate | - | assimilation |
43185 | 25115 | malate | - | assimilation |
43185 | 53258 | sodium citrate | - | assimilation |
43185 | 18401 | phenylacetate | - | assimilation |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
43185 | 15688 | acetoin | no |
43185 | 16136 | hydrogen sulfide | no |
43185 | 35581 | indole | no |
metabolite tests
@ref | Chebi-ID | metabolite | voges-proskauer-test | citrate test |
---|---|---|---|---|
43185 | 15688 | acetoin | - | |
43185 | 16947 | citrate | - |
enzymes
@ref | value | activity | ec |
---|---|---|---|
43185 | cytochrome oxidase | + | 1.9.3.1 |
43185 | catalase | + | 1.11.1.6 |
43185 | lysine decarboxylase | - | 4.1.1.18 |
43185 | ornithine decarboxylase | - | 4.1.1.17 |
43185 | tryptophan deaminase | - | 4.1.99.1 |
43185 | arginine dihydrolase | - | 3.5.3.6 |
43185 | beta-galactosidase | - | 3.2.1.23 |
43185 | gelatinase | - | |
43185 | urease | - | 3.5.1.5 |
fatty acid profile
fatty acids
@ref fatty acid percentage 43185 C18:1ω7c / C18:1ω6c 53.9 43185 C16:0 15.2 - type of FA analysis: whole cell analysis
- incubation medium: tryptic soy agar (TSA)
- agar/liquid: agar
- system: MIS MIDI
- cutoff value:
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent | enrichment culture | isolation procedure |
---|---|---|---|---|---|---|---|
42883 | soap-making tank of a household product plant | Beijing | China | CHN | Asia | ||
43185 | soap-making tank of a household product plant | China | CHN | Asia | trypticase soy agar (TSA, BD 236950) | serial dilution plating technique |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Engineered | #Built environment | #Container (Reservoir) |
#Engineered | #Industrial | #Plant (Factory) |
taxonmaps
- @ref: 69479
- File name: preview.99_11361.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_48;96_430;97_996;98_8492;99_11361&stattab=map
- Last taxonomy: Halomonas
- 16S sequence: KU530128
- Sequence Identity:
- Total samples: 2544
- soil counts: 292
- aquatic counts: 759
- animal counts: 1357
- plant counts: 136
Safety information
risk assessment
- @ref: 42883
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 42883
- description: Halomonas alkalicola strain CICC 11012s 16S ribosomal RNA gene, partial sequence
- accession: KU530128
- length: 1500
- database: ena
- NCBI tax ID: 1930622
GC content
- @ref: 42883
- GC-content: 63.2
- method: high performance liquid chromatography (HPLC)
External links
@ref: 42883
culture collection no.: DSM 103354, CICC 11012s
straininfo link
- @ref: 96582
- straininfo: 403902
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 28005522 | Halomonas alkalicola sp. nov., isolated from a household product plant. | Tang X, Zhai L, Lin Y, Yao S, Wang L, Ge Y, Liu Y, Zhang X, Zhang T, Zhang L, Liu J, Cheng C | Int J Syst Evol Microbiol | 10.1099/ijsem.0.001757 | 2017 | Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/chemistry, Genotype, Halomonas/*classification/genetics/isolation & purification, *Household Products, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry | Genetics |
Genetics | 29270851 | Genome sequencing and heterologous expression of antiporters reveal alkaline response mechanisms of Halomonas alkalicola. | Zhai L, Xie J, Lin Y, Cheng K, Wang L, Yue F, Guo J, Liu J, Yao S | Extremophiles | 10.1007/s00792-017-0991-6 | 2017 | Adaptation, Physiological, Bacterial Proteins/genetics/*metabolism, Extreme Environments, *Genome, Bacterial, Halomonas/*genetics, Potassium-Hydrogen Antiporters/genetics/*metabolism, Salinity, Sodium-Hydrogen Exchangers/genetics/*metabolism | Metabolism |
Phylogeny | 29948829 | Halomonas tibetensis sp. nov., isolated from saline lakes on Tibetan Plateau. | Lu HB, Xing P, Zhai L, Phurbu D, Tang Q, Wu QL | J Microbiol | 10.1007/s12275-018-8076-5 | 2018 | Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/analysis, Halomonas/chemistry/classification/*genetics/*isolation & purification, Lakes/*microbiology, Nucleic Acid Hybridization, Phospholipids/analysis, Phylogeny, Quinones/analysis, RNA, Ribosomal, 16S/genetics, *Salinity, Sequence Analysis, DNA, Tibet | Transcriptome |
Metabolism | 33123748 | Mechanism of TonB-dependent transport system in Halomonas alkalicola CICC 11012s in response to alkaline stress. | Zhai L, Xie J, Feng H, Sun S, Cheng K, Yao S | Extremophiles | 10.1007/s00792-020-01209-6 | 2020 | Alkalies, Bacterial Proteins/*genetics/metabolism, Biological Transport, Escherichia coli/metabolism, Halomonas/genetics/*metabolism, Hydrogen-Ion Concentration, Iron/*metabolism, Membrane Proteins/*genetics/metabolism, *Stress, Physiological | Stress |
Reference
@id | authors | title | doi/url | catalogue | journal | pubmed |
---|---|---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |||
42883 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-103354 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 103354) | |||
43185 | Xiaoli Tang, Lei Zhai, Yafang Lin, Su Yao, Lijiang Wang, Yuanyuan Ge, Yang Liu, Xin Zhang, Tianci Zhang, Lu Zhang, Jiquan Liu, Chi Cheng | Halomonas alkalicola sp. nov., isolated from a household product plant | 10.1099/ijsem.0.001757 | IJSEM 67: 1546-1550 2017 | 28005522 | |
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | |||
96582 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID403902.1 |