Strain identifier

BacDive ID: 140275

Type strain: Yes

Species: Luteitalea pratensis

Strain Designation: HEG_-6_39

Strain history: <- Selma Vieira, DSM

NCBI tax ID(s): 1855912 (species)

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General

@ref: 42882

BacDive-ID: 140275

DSM-Number: 100886

keywords: genome sequence, 16S sequence, Bacteria, aerobe, chemoorganotroph, Gram-negative, rod-shaped, colony-forming

description: Luteitalea pratensis HEG_-6_39 is an aerobe, chemoorganotroph, Gram-negative bacterium that forms circular colonies and was isolated from mown pasture grassland soil.

NCBI tax id

  • NCBI tax id: 1855912
  • Matching level: species

strain history

@refhistory
42882<- S. Vieira, Leibniz-Institut DSMZ GmbH, Braunschweig, Germany; HEG_6_39
67771<- Selma Vieira, DSM

doi: 10.13145/bacdive140275.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/acidobacteriota
  • domain: Bacteria
  • phylum: Acidobacteriota
  • class: Vicinamibacteria
  • order: Vicinamibacterales
  • family: Vicinamibacteraceae
  • genus: Luteitalea
  • species: Luteitalea pratensis
  • full scientific name: Luteitalea pratensis Vieira et al. 2017

@ref: 42882

domain: Bacteria

phylum: Acidobacteria

class: Vicinamibacteria

order: Vicinamibacterales

family: Vicinamibacteraceae

genus: Luteitalea

species: Luteitalea pratensis

full scientific name: Luteitalea pratensis Vieira et al. 2017

strain designation: HEG_-6_39

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
43190negative0.9-1.4 µm0.6-0.75 µmrod-shapedno
69480negative99.99

colony morphology

  • @ref: 43190
  • colony size: 0.5-1.0 mm
  • colony color: dark yellow
  • colony shape: circular
  • medium used: soil solution equivalent (SSE)/HD 1 : 10 medium

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
43190soil solution equivalent (SSE)/HD 1 : 10 mediumyes
42882SSE/HD1:10 (DSMZ Medium 1426)yeshttps://mediadive.dsmz.de/medium/1426Name: SSE/HD 1:10 MEDIUM (DSMZ Medium 1426) Composition: MES 1.95 g/l Peptone 0.5 g/l CaSO4 x 2 H2O 0.4303 g/l MgSO4 x 7 H2O 0.3695 g/l Yeast extract 0.25 g/l Na NO3 0.212 g/l CaCl2 x 2 H2O 0.1469 g/l Ca(NO3)2 x 4 H2O 0.118 g/l MgCl2 x 6 H2O 0.1018 g/l Glucose 0.1 g/l (NH4)2SO4 0.09915 g/l NH4Cl 0.05345 g/l K2SO4 0.0435 g/l FeSO4 x 7 H2O 0.00555 g/l KH2PO4 0.00340213 g/l HCl 0.0025 g/l FeCl2 x 4 H2O 0.0015 g/l CoCl2 x 6 H2O 0.00019 g/l MnCl2 x 4 H2O 0.0001 g/l ZnCl2 7e-05 g/l Na2MoO4 x 2 H2O 3.6e-05 g/l NiCl2 x 6 H2O 2.4e-05 g/l Pyridoxine hydrochloride 1e-05 g/l H3BO3 6e-06 g/l Lipoic acid 5e-06 g/l D-Calcium pantothenate 5e-06 g/l Nicotinic acid 5e-06 g/l Riboflavin 5e-06 g/l Thiamine-HCl x 2 H2O 5e-06 g/l p-Aminobenzoic acid 5e-06 g/l Folic acid 2e-06 g/l Biotin 2e-06 g/l CuCl2 x 2 H2O 2e-06 g/l Vitamin B12 1e-07 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
43190positiveoptimum20-30
43190positivegrowth0-36
42882positivegrowth28mesophilic
67771positivegrowth28mesophilic

culture pH

@refabilitytypepH
43190positivegrowth5.3-8.3
43190positiveoptimum6.1-7.5

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
43190aerobe
67771aerobe

nutrition type

  • @ref: 43190
  • type: chemoorganotroph

spore formation

@refspore formationconfidence
43190no
69481no100
69480no99.977

halophily

@refsaltgrowthtested relationconcentration
43190NaClpositivegrowth0-1 %(w/v)
43190NaClpositiveoptimum0.25 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4319017234glucose+carbon source
4319017716lactose+carbon source
4319017057cellobiose+carbon source
4319028260galactose+carbon source
4319037684mannose+carbon source
431906731melezitose+carbon source
4319016634raffinose+carbon source
4319017306maltose+carbon source
4319017992sucrose+carbon source
4319027082trehalose+carbon source
4319018222xylose+carbon source
43190casamino acids+carbon source
43190casein hydrolysate+carbon source
43190yeast extract+carbon source
4319015978glycerol 3-phosphate+carbon source
43190peptone+carbon source
4319024265gluconate+carbon source
4319028037N-acetylgalactosamine+carbon source
4319050144sodium pyruvate+carbon source
431902-oxogluconate+carbon source
4319036241protocatechuate+carbon source
4319035391aspartate+carbon source
4319028757fructose-growth
4319033984fucose-growth
4319027922sorbose-growth
4319025097lyxose-growth
4319026546rhamnose-growth
4319015963ribitol-growth
4319029864mannitol-growth
4319017268myo-inositol-growth
4319030911sorbitol-growth
4319017151xylitol-growth
4319025094lysine-growth
4319024741hydroxyproline-growth
4319029805glycolate-growth
4319015792malonate-growth
4319017272propionate-growth
4319016452oxaloacetate-growth
4319024996lactate-growth
4319028885butanol-growth
4319016236ethanol-growth
4319017790methanol-growth
4319028831propanol-growth
43190506227N-acetylglucosamine-growth
4319027689decanoate-growth
4319025646octanoate-growth
4319016813galactitol-growth
4319030742ethylene glycol-growth
4319033946erythrose-growth
4319023958erythrulose-growth
43190645522-hydroxybutyrate-growth
4319016087isocitrate-growth
43190391504-oxopentanoate-growth
4319022599arabinose-growth
431905417glucosamine-growth
4319024297glucuronate-growth
4319022605arabinitol-growth
4319035899crotonate-growth
43190309162-oxoglutarate-growth
4319015688acetoin-growth
4319022651ascorbate-growth
4319036655glyoxylate-growth
43190286442-oxopentanoate-growth
43190526821,2-butandiol-growth
43190620642,3-butanediol-growth
43190169971,2-propandiol-growth
4319016449alanine-growth
4319025017leucine-growth
4319029016arginine-growth
4319022653asparagine-growth
4319015356cysteine-growth
4319028300glutamine-growth
4319024898isoleucine-growth
4319018257ornithine-growth
4319026271proline-growth
4319016150benzoate-growth
4319027897tryptophan-growth
4319032954sodium acetate-growth
4319017968butyrate-growth
4319015740formate-growth
431908295beta-hydroxybutyrate-growth
43190167244-hydroxybutyrate-growth
4319048944isobutyrate-growth
4319018186tyrosine-growth
4319017822serine-growth
4319028044phenylalanine-growth
4319015428glycine-growth
4319027570histidine-growth
4319027266valine-growth
4319016811methionine-growth
4319026986threonine-growth
4319032544nicotinate-growth
4319053426tween 80-growth
431906364laminarin-growth
4319017128adipate-growth
4319030031succinate-growth
4319036208shikimate-growth
4319029987glutamate-growth
4319025115malate-growth
4319016947citrate-growth
4319030929tartrate-growth
43190fermented rumen extract-growth
4319048942isovalerate-growth
4319029806fumarate-growth
4319032362heptanoate-growth
43190trimethoxybenzoate-growth
4319017632nitrate-reduction
4319017234glucose-fermentation
431905291gelatin-hydrolysis
431904853esculin-hydrolysis
6836925115malate-assimilation
6836917128adipate-assimilation
6836927689decanoate-assimilation
6836924265gluconate-assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose-assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate+reduction

antibiotic resistance

@refChEBImetaboliteis antibioticis resistantresistance conc.
4319028971ampicillinyesyes10 µg (disc)
43190161680aztreonamyesyes30 µg (disc)
43190124991cefalotinyesyes30 µg (disc)
43190474053cefazolinyesyes30 µg (disc)
43190204928cefotaximeyesyes30 µg (disc)
4319017698chloramphenicolyesyes30 µg (disc)
4319048923erythromycinyesyes15 µg (disc)
4319028915fosfomycinyesyes50 µg (disc)
43190471744imipenemyesyes10 µg (disc)
4319063607linezolidyesyes30 µg (disc)
431906919mezlocillinyesyes30 µg (disc)
4319063611moxifloxacinyesyes5 µg (disc)
431907507neomycinyesyes30 µg (disc)
4319071415nitrofurantoinyesyes100 µg (disc)
43190100246norfloxacinyesyes10 µg (disc)
431907660nystatinyesyes100 Unit (disc)
431907731ofloxacinyesyes5 µg (disc)
431907809oxacillinyesyes5 µg (disc)
4319018208penicillin gyesyes10 Unit (disc)
4319075250pipemidic acidyesyes20 µg (disc)
431909587ticarcillinyesyes75 µg (disc)

metabolite production

@refChebi-IDmetaboliteproduction
4319035581indoleno
6836935581indoleno

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
43190catalase+1.11.1.6
43190cytochrome oxidase-1.9.3.1
43190alkaline phosphatase+3.1.3.1
43190acid phosphatase+3.1.3.2
43190esterase (C 4)+
43190trypsin+3.4.21.4
43190alpha-chymotrypsin+3.4.21.1
43190naphthol-AS-BI-phosphohydrolase+
43190esterase Lipase (C 8)+
43190leucine arylamidase+3.4.11.1
43190lipase-
43190valine arylamidase-
43190cystine arylamidase-3.4.11.3
43190alpha-galactosidase-3.2.1.22
43190beta-galactosidase-3.2.1.23
43190beta-glucuronidase-3.2.1.31
43190alpha-glucosidase-3.2.1.20
43190N-acetyl-beta-glucosaminidase-3.2.1.52
43190alpha-mannosidase-3.2.1.24
43190alpha-fucosidase-3.2.1.51
43190arginine dihydrolase-3.5.3.6
43190urease-3.5.1.5
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)-
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382alpha-chymotrypsin-3.4.21.1
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    43190C15:0 iso30.3
    43190C16:1ω6c / C16:1ω7c18.4
    43190C18:1ω9c17.1
    43190C17:1 iso ω9c15.7
    43190C17:0 iso5
    43190C18:02.8
    43190C16:02.2
  • type of FA analysis: whole cell analysis
  • incubation medium: SSE/HD1 : 10 medium (pH 7.0)
  • agar/liquid: liquid
  • incubation temperature: 28
  • incubation time: 10
  • software version: Sherlock 6.1
  • system: MIS MIDI

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
42882-+---+/---+/--+/-+/---------

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPAC
42882+------+/-------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudeenrichment cultureenrichment culture compositionenrichment culture durationenrichment culture temperatureisolation procedure
42882mown pasture grassland soilThuringia, Hainich-Dün region close to Mühlhausen (51° 12' 53.971'' N 10° 23' 27.992'' E)GermanyDEUEurope51.21510.3911
43190soil sample from a temperate grassland soilMühlhausen, ThuringiaGermanyDEUEurope5110SSE/HP (pH 7.0) platessoil solution equivalent (SSE), supplemented with peptone, yeast extract, vitamin solution, trace element solution, 0.8 % (w/v) gellan gum4 monthsroom temperatureserial dilution, darkness
67771From soil, Grassland soil from Heinich-DunThuringenGermanyDEUEurope

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Grassland
#Environmental#Terrestrial#Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_42214.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_723;96_3851;97_4747;98_6098;99_42214&stattab=map
  • Last taxonomy: Luteitalea pratensis subclade
  • 16S sequence: KT287072
  • Sequence Identity:
  • Total samples: 543
  • soil counts: 353
  • aquatic counts: 44
  • animal counts: 90
  • plant counts: 56

Safety information

risk assessment

  • @ref: 42882
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 42882
  • description: Luteitalea pratensis strain HEG_-6_39 16S ribosomal RNA gene, partial sequence
  • accession: KT287072
  • length: 1506
  • database: ena
  • NCBI tax ID: 1855912

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Luteitalea pratensis DSM 100886GCA_001618865completencbi1855912
66792Luteitalea pratensis strain DSM 100886; HEG_-6_391855912.4wgspatric1855912
66792Luteitalea pratensis DSM 1008862687453111completeimg1855912

GC content

@refGC-contentmethod
4319064.7genome sequence analysis
4288264.7sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
flagellatedno89.373yes
gram-positiveno96.068no
anaerobicno97.378yes
aerobicyes74.198no
halophileno92.152no
spore-formingno91.599yes
glucose-utilyes73.325no
motileyes62.121no
thermophileno98.73no
glucose-fermentno88.498yes

External links

@ref: 42882

culture collection no.: DSM 100886, KCTC 52215

straininfo link

  • @ref: 96581
  • straininfo: 403387

literature

  • topic: Phylogeny
  • Pubmed-ID: 28141504
  • title: Luteitalea pratensis gen. nov., sp. nov. a new member of subdivision 6 Acidobacteria isolated from temperate grassland soil.
  • authors: Vieira S, Luckner M, Wanner G, Overmann J
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001827
  • year: 2017
  • mesh: Acidobacteria/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Germany, *Grassland, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
42882Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-100886Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 100886)
43190Selma Vieira, Manja Luckner, Gerhard Wanner, Jörg OvermannLuteitalea pratensis gen. nov., sp. nov. a new member of subdivision 6 Acidobacteria isolated from temperate grassland soil10.1099/ijsem.0.001827IJSEM 67: 1408-1414 201728141504
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar SchomburgBRENDA, the ELIXIR core data resource in 2021: new developments and updates10.1093/nar/gkaa1025Nucleic Acids Res. 49: D498-D508 202033211880
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
68369Automatically annotated from API 20NE
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
96581Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID403387.1