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Strain identifier

BacDive ID: 140267

Type strain: Yes

Species: Fournierella massiliensis

Strain Designation: AT2

Strain history: <- S. Khelaifia, URMITE, Univ. Méditerranée, Marseille, France; AT2

NCBI tax ID(s): 1650663 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 42874

BacDive-ID: 140267

DSM-Number: 100451

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, thermophilic, Gram-negative, rod-shaped

description: Fournierella massiliensis AT2 is an anaerobe, thermophilic, Gram-negative bacterium that was isolated from human stool specimen, healthy 28-year-old French man.

NCBI tax id

  • NCBI tax id: 1650663
  • Matching level: species

strain history: <- S. Khelaifia, URMITE, Univ. Méditerranée, Marseille, France; AT2

doi: 10.13145/bacdive140267.20221219.7.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Oscillospiraceae
  • genus: Fournierella
  • species: Fournierella massiliensis
  • full scientific name: Fournierella massiliensis Togo et al. 2017

@ref: 42874

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Eubacteriales

family: Oscillospiraceae

genus: Fournierella

species: Fournierella massiliensis

full scientific name: Fournierella massiliensis Togo et al. 2017

strain designation: AT2

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
43184negative2 µm0.5 µmrod-shapedno
69480positive81.211

colony morphology

  • @ref: 43184
  • colony size: 0.3-1 mm
  • colony color: white
  • medium used: 5 % sheep blood-enriched Columbia agar

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
431845 % sheep blood-enriched Columbia agaryes
42874WILKINS-CHALGREN ANAEROBE BROTH (DSMZ Medium 339)yeshttps://mediadive.dsmz.de/medium/339Name: WILKINS-CHALGREN ANAEROBE BROTH (DSMZ Medium 339) Composition: dehydrated Wilkins-Chalgren medium 33.0 g/l L-Cysteine HCl 0.3 g/l Sodium resazurin 0.0005 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
43184nogrowth55thermophilic
43184positivegrowth45thermophilic
43184positiveoptimum37mesophilic
43184positivegrowth28mesophilic
42874positivegrowth37mesophilic

culture pH

@refabilitytypepHPH range
43184positivegrowth6-8.5alkaliphile
43184positiveoptimum7.0-7.3

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
43184anaerobe
69480anaerobe98.706

spore formation

@refspore formationconfidence
43184no
69480no95.075

halophily

@refsaltgrowthtested relationconcentrationhalophily levelconfidence
43184NaClnogrowth10 g/L
69480non-halophilic98.479

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4318417754glycerol+builds acid from
4318412936D-galactose+builds acid from
4318417634D-glucose+builds acid from
4318415824D-fructose+builds acid from
4318416024D-mannose+builds acid from
43184methyl alpha-D-glucopyranoside+builds acid from
43184esculin ferric citrate+builds acid from
4318417814salicin+builds acid from
4318417306maltose+builds acid from
4318417716lactose+builds acid from
4318428053melibiose+builds acid from
4318417992sucrose+builds acid from
431846731melezitose+builds acid from
4318416634raffinose+builds acid from
4318432528turanose+builds acid from
43184potassium 5-dehydro-D-gluconate+builds acid from
4318417113erythritol-builds acid from
4318417108D-arabinose-builds acid from
4318430849L-arabinose-builds acid from
4318416988D-ribose-builds acid from
4318465328L-xylose-builds acid from
4318415963ribitol-builds acid from
4318474863methyl beta-D-xylopyranoside-builds acid from
4318417266L-sorbose-builds acid from
4318462345L-rhamnose-builds acid from
4318416813galactitol-builds acid from
4318417268myo-inositol-builds acid from
4318416899D-mannitol-builds acid from
4318417924D-sorbitol-builds acid from
4318443943methyl alpha-D-mannoside-builds acid from
43184506227N-acetylglucosamine-builds acid from
4318427613amygdalin-builds acid from
4318418305arbutin-builds acid from
4318417057cellobiose-builds acid from
4318427082trehalose-builds acid from
4318415443inulin-builds acid from
4318428017starch-builds acid from
4318428087glycogen-builds acid from
4318417151xylitol-builds acid from
4318428066gentiobiose-builds acid from
4318465327D-xylose-builds acid from
4318416443D-tagatose-builds acid from
4318428847D-fucose-builds acid from
4318418287L-fucose-builds acid from
4318418333D-arabitol-builds acid from
4318418403L-arabitol-builds acid from
4318432032potassium gluconate-builds acid from
43184potassium 2-dehydro-D-gluconate-builds acid from
4318417634D-glucose+assimilation
4318417992sucrose+assimilation
4318416024D-mannose+assimilation
4318417716lactose+assimilation
43184esculin ferric citrate+assimilation
4318417814salicin+assimilation
4318417306maltose+assimilation
431846731melezitose+assimilation
4318417754glycerol+assimilation
4318416634raffinose+assimilation
4318416899D-mannitol-assimilation
4318465327D-xylose-assimilation
4318462968cellulose-assimilation
4318417924D-sorbitol-assimilation
4318430849L-arabinose-assimilation
4318462345L-rhamnose-assimilation
4318427082trehalose-assimilation
431845291gelatin-hydrolysis
6838027897tryptophan-energy source
6838017632nitrate-reduction
6838029985L-glutamate-degradation
6838016634raffinose-fermentation
6838016024D-mannose-fermentation
6838029016arginine-hydrolysis
6838016199urea-hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
4318435581indoleno
6838035581indoleno

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
43184urease-3.5.1.5
43184esterase (C 4)+
43184esterase Lipase (C 8)+
43184acid phosphatase+3.1.3.2
43184naphthol-AS-BI-phosphohydrolase+
43184alpha-galactosidase+3.2.1.22
43184alpha-glucosidase+3.2.1.20
43184beta-glucosidase+3.2.1.21
43184N-acetyl-beta-glucosaminidase+3.2.1.52
43184alkaline phosphatase-3.1.3.1
43184lipase-
43184leucine arylamidase-3.4.11.1
43184valine arylamidase-
43184cystine arylamidase-3.4.11.3
43184trypsin-3.4.21.4
43184alpha-chymotrypsin-3.4.21.1
43184beta-galactosidase-3.2.1.23
43184beta-glucuronidase-3.2.1.31
43184alpha-mannosidase-3.2.1.24
43184alpha-fucosidase-3.2.1.51
68380serine arylamidase-
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase-
68380glycin arylamidase-
68380alanine arylamidase-3.4.11.2
68380tyrosine arylamidase-
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase-3.4.11.1
68380phenylalanine arylamidase-
68380leucyl glycin arylamidase-3.4.11.1
68380proline-arylamidase-3.4.11.5
68380L-arginine arylamidase-
68380alkaline phosphatase-3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase+3.2.1.21
68380alpha-glucosidase+3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase+3.2.1.23
68380alpha-galactosidase+3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
42874--++-++----------------------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentenrichment cultureenrichment culture durationenrichment culture temperature
42874human stool specimen, healthy 28-year-old French manFranceFRAEurope
43184fresh stool sample from a healthy 28-year-old French man5 % sheep blood-enriched Columbia agar (BioMerieux)2 days37

isolation source categories

Cat1Cat2Cat3
#Infection#Patient#Specimen
#Host Body Product#Gastrointestinal tract#Feces (Stool)
#Host#Human#Male

taxonmaps

  • @ref: 69479
  • File name: preview.99_6553.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_286;96_3106;97_3807;98_4837;99_6553&stattab=map
  • Last taxonomy: Fournierella massiliensis subclade
  • 16S sequence: LN846908
  • Sequence Identity:
  • Total samples: 55536
  • soil counts: 282
  • aquatic counts: 964
  • animal counts: 54181
  • plant counts: 109

Safety information

risk assessment

  • @ref: 42874
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 42874
  • description: Ruminococcaceae bacterium AT2 partial 16S rRNA gene, strain AT2
  • accession: LN846908
  • length: 1491
  • database: ena
  • NCBI tax ID: 1650663

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Fournierella massiliensis DSM 100451GCA_004345265contigncbi1650663
66792Fournierella massiliensis strain DSM 1004511650663.6wgspatric1650663
66792Fournierella massiliensis DSM 1004512788499850draftimg1650663
66792Bacillus anthracis delta ANR S3-18GCA_009872985scaffoldpatric1392

GC content

@refGC-contentmethod
4318456.8genome sequence analysis
4287456.8sequence analysis

External links

@ref: 42874

culture collection no.: DSM 100451, CSUR P2014

literature

  • topic: Phylogeny
  • Pubmed-ID: 28126042
  • title: Fournierella massiliensis gen. nov., sp. nov., a new human-associated member of the family Ruminococcaceae.
  • authors: Togo AH, Durand G, Khelaifia S, Armstrong N, Robert C, Cadoret F, Di Pinto F, Delerce J, Levasseur A, Raoult D, Million M
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001826
  • year: 2017
  • mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Feces/*microbiology, France, Gram-Positive Bacteria/*classification/genetics/isolation & purification, Humans, Male, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA
  • topic2: Pathogenicity

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
42874Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-100451Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 100451)
43184Amadou Hamidou Togo, Guillaume Durand, Saber Khelaifia, Nicholas Armstrong, Catherine Robert, Frederic Cadoret, Fabrizio Di Pinto, Jeremy Delerce, Anthony Levasseur, Didier Raoult, Matthieu MillionFournierella massiliensis gen. nov., sp. nov., a new human-associated member of the family Ruminococcaceae10.1099/ijsem.0.001826IJSEM 67: 1393-1399 201728126042
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68380Automatically annotated from API rID32A
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes