Strain identifier
BacDive ID: 140267
Type strain:
Species: Fournierella massiliensis
Strain Designation: AT2
Strain history: <- S. Khelaifia, URMITE, Univ. Méditerranée, Marseille, France; AT2
NCBI tax ID(s): 1650663 (species)
General
@ref: 42874
BacDive-ID: 140267
DSM-Number: 100451
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, thermophilic, Gram-negative, rod-shaped
description: Fournierella massiliensis AT2 is an anaerobe, thermophilic, Gram-negative bacterium that was isolated from human stool specimen, healthy 28-year-old French man.
NCBI tax id
- NCBI tax id: 1650663
- Matching level: species
strain history
- @ref: 42874
- history: <- S. Khelaifia, URMITE, Univ. Méditerranée, Marseille, France; AT2
doi: 10.13145/bacdive140267.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Clostridia
- order: Eubacteriales
- family: Oscillospiraceae
- genus: Fournierella
- species: Fournierella massiliensis
- full scientific name: Fournierella massiliensis Togo et al. 2017
@ref: 42874
domain: Bacteria
phylum: Firmicutes
class: Clostridia
order: Eubacteriales
family: Oscillospiraceae
genus: Fournierella
species: Fournierella massiliensis
full scientific name: Fournierella massiliensis Togo et al. 2017
strain designation: AT2
type strain: yes
Morphology
cell morphology
- @ref: 43184
- gram stain: negative
- cell length: 2 µm
- cell width: 0.5 µm
- cell shape: rod-shaped
- motility: no
colony morphology
- @ref: 43184
- colony size: 0.3-1 mm
- colony color: white
- medium used: 5 % sheep blood-enriched Columbia agar
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
43184 | 5 % sheep blood-enriched Columbia agar | yes | ||
42874 | WILKINS-CHALGREN ANAEROBE BROTH (DSMZ Medium 339) | yes | https://mediadive.dsmz.de/medium/339 | Name: WILKINS-CHALGREN ANAEROBE BROTH (DSMZ Medium 339) Composition: dehydrated Wilkins-Chalgren medium 33.0 g/l L-Cysteine HCl 0.3 g/l Sodium resazurin 0.0005 g/l Distilled water |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
43184 | no | growth | 55 | thermophilic |
43184 | positive | growth | 45 | thermophilic |
43184 | positive | optimum | 37 | mesophilic |
43184 | positive | growth | 28 | mesophilic |
42874 | positive | growth | 37 | mesophilic |
culture pH
@ref | ability | type | pH | PH range |
---|---|---|---|---|
43184 | positive | growth | 6-8.5 | alkaliphile |
43184 | positive | optimum | 7.0-7.3 |
Physiology and metabolism
oxygen tolerance
- @ref: 43184
- oxygen tolerance: anaerobe
spore formation
- @ref: 43184
- spore formation: no
halophily
- @ref: 43184
- salt: NaCl
- growth: no
- tested relation: growth
- concentration: 10 g/L
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
43184 | 17754 | glycerol | + | builds acid from |
43184 | 12936 | D-galactose | + | builds acid from |
43184 | 17634 | D-glucose | + | builds acid from |
43184 | 15824 | D-fructose | + | builds acid from |
43184 | 16024 | D-mannose | + | builds acid from |
43184 | methyl alpha-D-glucopyranoside | + | builds acid from | |
43184 | esculin ferric citrate | + | builds acid from | |
43184 | 17814 | salicin | + | builds acid from |
43184 | 17306 | maltose | + | builds acid from |
43184 | 17716 | lactose | + | builds acid from |
43184 | 28053 | melibiose | + | builds acid from |
43184 | 17992 | sucrose | + | builds acid from |
43184 | 6731 | melezitose | + | builds acid from |
43184 | 16634 | raffinose | + | builds acid from |
43184 | 32528 | turanose | + | builds acid from |
43184 | potassium 5-dehydro-D-gluconate | + | builds acid from | |
43184 | 17113 | erythritol | - | builds acid from |
43184 | 17108 | D-arabinose | - | builds acid from |
43184 | 30849 | L-arabinose | - | builds acid from |
43184 | 16988 | D-ribose | - | builds acid from |
43184 | 65328 | L-xylose | - | builds acid from |
43184 | 15963 | ribitol | - | builds acid from |
43184 | 74863 | methyl beta-D-xylopyranoside | - | builds acid from |
43184 | 17266 | L-sorbose | - | builds acid from |
43184 | 62345 | L-rhamnose | - | builds acid from |
43184 | 16813 | galactitol | - | builds acid from |
43184 | 17268 | myo-inositol | - | builds acid from |
43184 | 16899 | D-mannitol | - | builds acid from |
43184 | 17924 | D-sorbitol | - | builds acid from |
43184 | 43943 | methyl alpha-D-mannoside | - | builds acid from |
43184 | 506227 | N-acetylglucosamine | - | builds acid from |
43184 | 27613 | amygdalin | - | builds acid from |
43184 | 18305 | arbutin | - | builds acid from |
43184 | 17057 | cellobiose | - | builds acid from |
43184 | 27082 | trehalose | - | builds acid from |
43184 | 15443 | inulin | - | builds acid from |
43184 | 28017 | starch | - | builds acid from |
43184 | 28087 | glycogen | - | builds acid from |
43184 | 17151 | xylitol | - | builds acid from |
43184 | 28066 | gentiobiose | - | builds acid from |
43184 | 65327 | D-xylose | - | builds acid from |
43184 | 16443 | D-tagatose | - | builds acid from |
43184 | 28847 | D-fucose | - | builds acid from |
43184 | 18287 | L-fucose | - | builds acid from |
43184 | 18333 | D-arabitol | - | builds acid from |
43184 | 18403 | L-arabitol | - | builds acid from |
43184 | 32032 | potassium gluconate | - | builds acid from |
43184 | potassium 2-dehydro-D-gluconate | - | builds acid from | |
43184 | 17634 | D-glucose | + | assimilation |
43184 | 17992 | sucrose | + | assimilation |
43184 | 16024 | D-mannose | + | assimilation |
43184 | 17716 | lactose | + | assimilation |
43184 | esculin ferric citrate | + | assimilation | |
43184 | 17814 | salicin | + | assimilation |
43184 | 17306 | maltose | + | assimilation |
43184 | 6731 | melezitose | + | assimilation |
43184 | 17754 | glycerol | + | assimilation |
43184 | 16634 | raffinose | + | assimilation |
43184 | 16899 | D-mannitol | - | assimilation |
43184 | 65327 | D-xylose | - | assimilation |
43184 | 62968 | cellulose | - | assimilation |
43184 | 17924 | D-sorbitol | - | assimilation |
43184 | 30849 | L-arabinose | - | assimilation |
43184 | 62345 | L-rhamnose | - | assimilation |
43184 | 27082 | trehalose | - | assimilation |
43184 | 5291 | gelatin | - | hydrolysis |
68380 | 16199 | urea | - | hydrolysis |
68380 | 29016 | arginine | - | hydrolysis |
68380 | 16024 | D-mannose | - | fermentation |
68380 | 16634 | raffinose | - | fermentation |
68380 | 29985 | L-glutamate | - | degradation |
68380 | 17632 | nitrate | - | reduction |
68380 | 27897 | tryptophan | - | energy source |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
43184 | 35581 | indole | no |
68380 | 35581 | indole | no |
metabolite tests
- @ref: 68380
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
@ref | value | activity | ec |
---|---|---|---|
43184 | urease | - | 3.5.1.5 |
43184 | esterase (C 4) | + | |
43184 | esterase Lipase (C 8) | + | |
43184 | acid phosphatase | + | 3.1.3.2 |
43184 | naphthol-AS-BI-phosphohydrolase | + | |
43184 | alpha-galactosidase | + | 3.2.1.22 |
43184 | alpha-glucosidase | + | 3.2.1.20 |
43184 | beta-glucosidase | + | 3.2.1.21 |
43184 | N-acetyl-beta-glucosaminidase | + | 3.2.1.52 |
43184 | alkaline phosphatase | - | 3.1.3.1 |
43184 | lipase | - | |
43184 | leucine arylamidase | - | 3.4.11.1 |
43184 | valine arylamidase | - | |
43184 | cystine arylamidase | - | 3.4.11.3 |
43184 | trypsin | - | 3.4.21.4 |
43184 | alpha-chymotrypsin | - | 3.4.21.1 |
43184 | beta-galactosidase | - | 3.2.1.23 |
43184 | beta-glucuronidase | - | 3.2.1.31 |
43184 | alpha-mannosidase | - | 3.2.1.24 |
43184 | alpha-fucosidase | - | 3.2.1.51 |
68380 | serine arylamidase | - | |
68380 | glutamyl-glutamate arylamidase | - | |
68380 | histidine arylamidase | - | |
68380 | glycin arylamidase | - | |
68380 | alanine arylamidase | - | 3.4.11.2 |
68380 | tyrosine arylamidase | - | |
68380 | pyrrolidonyl arylamidase | - | 3.4.19.3 |
68380 | leucine arylamidase | - | 3.4.11.1 |
68380 | phenylalanine arylamidase | - | |
68380 | leucyl glycin arylamidase | - | 3.4.11.1 |
68380 | proline-arylamidase | - | 3.4.11.5 |
68380 | L-arginine arylamidase | - | |
68380 | alkaline phosphatase | - | 3.1.3.1 |
68380 | tryptophan deaminase | - | 4.1.99.1 |
68380 | alpha-fucosidase | - | 3.2.1.51 |
68380 | glutamate decarboxylase | - | 4.1.1.15 |
68380 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68380 | beta-glucuronidase | - | 3.2.1.31 |
68380 | alpha-arabinosidase | - | 3.2.1.55 |
68380 | beta-glucosidase | + | 3.2.1.21 |
68380 | alpha-glucosidase | + | 3.2.1.20 |
68380 | beta-Galactosidase 6-phosphate | - | |
68380 | beta-galactosidase | + | 3.2.1.23 |
68380 | alpha-galactosidase | + | 3.2.1.22 |
68380 | arginine dihydrolase | - | 3.5.3.6 |
68380 | urease | - | 3.5.1.5 |
API rID32A
@ref | URE | ADH Arg | alpha GAL | beta GAL | beta GP | alpha GLU | beta GLU | alpha ARA | beta GUR | beta NAG | MNE | RAF | GDC | alpha FUC | NIT | IND | PAL | ArgA | ProA | LGA | PheA | LeuA | PyrA | TyrA | AlaA | GlyA | HisA | GGA | SerA |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
42874 | - | - | + | + | - | + | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | country | origin.country | continent | enrichment culture | enrichment culture duration | enrichment culture temperature |
---|---|---|---|---|---|---|---|
42874 | human stool specimen, healthy 28-year-old French man | France | FRA | Europe | |||
43184 | fresh stool sample from a healthy 28-year-old French man | 5 % sheep blood-enriched Columbia agar (BioMerieux) | 2 days | 37 |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Infection | #Patient | #Specimen |
#Host Body Product | #Gastrointestinal tract | #Feces (Stool) |
#Host | #Human | #Male |
taxonmaps
- @ref: 69479
- File name: preview.99_6553.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_286;96_3106;97_3807;98_4837;99_6553&stattab=map
- Last taxonomy: Fournierella massiliensis subclade
- 16S sequence: LN846908
- Sequence Identity:
- Total samples: 55536
- soil counts: 282
- aquatic counts: 964
- animal counts: 54181
- plant counts: 109
Safety information
risk assessment
- @ref: 42874
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 42874
- description: Ruminococcaceae bacterium AT2 partial 16S rRNA gene, strain AT2
- accession: LN846908
- length: 1491
- database: ena
- NCBI tax ID: 1650663
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Fournierella massiliensis DSM 100451 | GCA_004345265 | contig | ncbi | 1650663 |
66792 | Fournierella massiliensis strain DSM 100451 | 1650663.6 | wgs | patric | 1650663 |
66792 | Fournierella massiliensis DSM 100451 | 2788499850 | draft | img | 1650663 |
GC content
@ref | GC-content | method |
---|---|---|
43184 | 56.8 | genome sequence analysis |
42874 | 56.8 | sequence analysis |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
motile | no | 91.42 | yes |
gram-positive | yes | 86.198 | no |
anaerobic | yes | 99.583 | no |
aerobic | no | 98.695 | yes |
halophile | no | 85.57 | no |
spore-forming | no | 91.002 | yes |
thermophile | no | 95.126 | no |
glucose-util | yes | 87.918 | yes |
flagellated | no | 96.319 | no |
glucose-ferment | yes | 76.668 | no |
External links
@ref: 42874
culture collection no.: DSM 100451, CSUR P2014
straininfo link
- @ref: 96573
- straininfo: 403978
literature
- topic: Phylogeny
- Pubmed-ID: 28126042
- title: Fournierella massiliensis gen. nov., sp. nov., a new human-associated member of the family Ruminococcaceae.
- authors: Togo AH, Durand G, Khelaifia S, Armstrong N, Robert C, Cadoret F, Di Pinto F, Delerce J, Levasseur A, Raoult D, Million M
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijsem.0.001826
- year: 2017
- mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Feces/*microbiology, France, Gram-Positive Bacteria/*classification/genetics/isolation & purification, Humans, Male, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA
- topic2: Pathogenicity
Reference
@id | authors | title | doi/url | catalogue | journal | pubmed |
---|---|---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |||
42874 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-100451 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 100451) | |||
43184 | Amadou Hamidou Togo, Guillaume Durand, Saber Khelaifia, Nicholas Armstrong, Catherine Robert, Frederic Cadoret, Fabrizio Di Pinto, Jeremy Delerce, Anthony Levasseur, Didier Raoult, Matthieu Million | Fournierella massiliensis gen. nov., sp. nov., a new human-associated member of the family Ruminococcaceae | 10.1099/ijsem.0.001826 | IJSEM 67: 1393-1399 2017 | 28126042 | |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |||
68380 | Automatically annotated from API rID32A | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | |||
96573 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID403978.1 |