Strain identifier

BacDive ID: 140266

Type strain: Yes

Species: Mesobacillus zeae

Strain Designation: JJ-247

Strain history: <- P. Kämpfer, Justus-Liebig-Universität, Giessen, Germany <- J. A. McInroy; JJ-247

NCBI tax ID(s): 1917180 (species)

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General

@ref: 42873

BacDive-ID: 140266

DSM-Number: 103964

keywords: genome sequence, 16S sequence, Bacteria, aerobe, thermophilic, Gram-positive, rod-shaped, colony-forming

description: Mesobacillus zeae JJ-247 is an aerobe, thermophilic, Gram-positive bacterium that forms circular colonies and was isolated from rhizosphere of field-grown Zea mays plants.

NCBI tax id

  • NCBI tax id: 1917180
  • Matching level: species

strain history

  • @ref: 42873
  • history: <- P. Kämpfer, Justus-Liebig-Universität, Giessen, Germany <- J. A. McInroy; JJ-247

doi: 10.13145/bacdive140266.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Mesobacillus
  • species: Mesobacillus zeae
  • full scientific name: Mesobacillus zeae (Kämpfer et al. 2017) Patel and Gupta 2020

  • synonyms

    • @ref: 20215
    • synonym: Bacillus zeae

@ref: 42873

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Mesobacillus

species: Mesobacillus zeae

full scientific name: Mesobacillus zeae (Kämpfer et al. 2017) Patel and Gupta 2020

strain designation: JJ-247

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
43173positive3.5 µm1 µmrod-shapedno
69480yes98.632
69480positive100

colony morphology

  • @ref: 43173
  • colony color: beige
  • colony shape: circular
  • medium used: nutritient agar (NA)

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
43173nutritient agar (NA)yes
43173brain heart infusion agaryes
43173tryptic soy agar (TSA)yes
43173Reasoner's 2A agar (R2A)yes
42873CASO AGAR (MERCK 105458) (DSMZ Medium 220)yeshttps://mediadive.dsmz.de/medium/220Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
42873PLATE COUNT AGAR (DSMZ Medium 464)yeshttps://mediadive.dsmz.de/medium/464Name: PLATE COUNT AGAR (DSMZ Medium 464) Composition: Agar 15.0 g/l Tryptone 5.0 g/l Yeast extract 2.5 g/l Dextrose 1.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
43173positivemaximum50thermophilic
43173positiveminimum10psychrophilic
43173positiveoptimum28-30mesophilic
43173positivegrowth20-30
42873positivegrowth30mesophilic

culture pH

@refabilitytypepHPH range
43173positivegrowth6.0-10.0alkaliphile
43173positiveoptimum7

Physiology and metabolism

oxygen tolerance

  • @ref: 43173
  • oxygen tolerance: aerobe

spore formation

@refspore formationconfidence
43173no
69480yes99.999

halophily

@refsaltgrowthtested relationconcentration
43173NaClpositivegrowth0-8.0 %(w/v)
43173NaClpositiveoptimum3-5 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4317317634D-glucose+builds acid from
4317317992sucrose+builds acid from
4317317306maltose-builds acid from
4317327082trehalose-builds acid from
4317330849L-arabinose-builds acid from
4317315963ribitol-builds acid from
4317318333D-arabitol-builds acid from
4317316813galactitol-builds acid from
4317317113erythritol-builds acid from
4317317268myo-inositol-builds acid from
4317317716lactose-builds acid from
4317316899D-mannitol-builds acid from
4317328053melibiose-builds acid from
431733-O-methyl alpha-D-glucopyranoside-builds acid from
4317316634raffinose-builds acid from
4317362345L-rhamnose-builds acid from
4317317814salicin-builds acid from
4317317924D-sorbitol-builds acid from
4317365327D-xylose-builds acid from
43173casein-hydrolysis
431735291gelatin-hydrolysis
4317328017starch-hydrolysis
4317316991dna-hydrolysis
4317318186tyrosine-hydrolysis
4317330089acetate+carbon source
4317317272propionate+carbon source
4317317634D-glucose+carbon source
4317328037N-acetylgalactosamine-carbon source
43173506227N-acetylglucosamine-carbon source
4317330849L-arabinose-carbon source
4317317057cellobiose-carbon source
4317312936D-galactose-carbon source
4317317306maltose-carbon source
4317324265gluconate-carbon source
4317316024D-mannose-carbon source
4317315824D-fructose-carbon source
4317317754glycerol-carbon source
4317316899D-mannitol-carbon source
4317368428maltitol-carbon source
4317328053melibiose-carbon source
4317362345L-rhamnose-carbon source
4317316988D-ribose-carbon source
4317317814salicin-carbon source
4317317992sucrose-carbon source
4317365327D-xylose-carbon source
4317315963ribitol-carbon source
4317317268myo-inositol-carbon source
4317317924D-sorbitol-carbon source
4317317148putrescine-carbon source
4317316383cis-aconitate-carbon source
4317315708trans-aconitate-carbon source
4317316865gamma-aminobutyric acid-carbon source
4317317128adipate-carbon source
4317378208azelaate-carbon source
4317329806fumarate-carbon source
4317330921glutarate-carbon source
43173370543-hydroxybutyrate-carbon source
4317317240itaconate-carbon source
4317324996lactate-carbon source
43173309162-oxoglutarate-carbon source
4317315361pyruvate-carbon source
431739300suberic acid-carbon source
4317316947citrate-carbon source
4317336986mesaconate-carbon source
4317316977L-alanine-carbon source
4317316958beta-alanine-carbon source
4317315729L-ornithine-carbon source
4317317295L-phenylalanine-carbon source
4317317115L-serine-carbon source
4317329991L-aspartate-carbon source
4317315971L-histidine-carbon source
4317315603L-leucine-carbon source
4317317203L-proline-carbon source
4317316828L-tryptophan-carbon source
43173161933-hydroxybenzoate-carbon source
43173178794-hydroxybenzoate-carbon source
4317318401phenylacetate-carbon source
43173911224-nitrophenyl alpha-D-glucopyranoside+hydrolysis
431733122bis-4-nitrophenyl phosphate+hydrolysis
4317355394bis-4-nitrophenyl-phosphorylcholine-hydrolysis
431732-deoxythymidine-5'-4-nitrophenyl phosphate-hydrolysis
43173L-alanine 4-nitroanilide-hydrolysis
43173gamma-L-glutamate-4-nitroanilide-hydrolysis
43173L-proline-4-nitroanilide-hydrolysis
43173902594-nitrophenyl beta-D-glucopyranoside-hydrolysis
431733557154-nitrophenyl beta-D-galactopyranoside-hydrolysis
43173901484-nitrophenyl beta-D-xylopyranoside-hydrolysis
43173901464-nitrophenyl beta-D-glucuronide-hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
4317335581indoleno
4317316136hydrogen sulfideno

enzymes

@refvalueactivityec
43173cytochrome oxidase+1.9.3.1
43173catalase+1.11.1.6
43173urease-3.5.1.5
43173arginine dihydrolase-3.5.3.6
43173lysine decarboxylase-4.1.1.18
43173ornithine decarboxylase-4.1.1.17
43173beta-galactosidase-3.2.1.23

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    43173C14:0 iso2.5
    43173C15:0 iso41.9
    43173C15:1 iso F1.5
    43173C15:0 anteiso4.8
    43173C15:02.7
    43173C16:1ω7c OH4.9
    43173C16:0 iso7.7
    43173C16:1ω11c3.6
    43173C16:03.1
    43173C17:1 iso ω10c9.3
    43173iso-C17:1 l / anteiso-C17:1 B4.3
    43173C17:0 iso9.1
    43173C17:0 anteiso4.5
  • type of FA analysis: whole cell analysis
  • incubation medium: CASO (Carl Roth)
  • agar/liquid: agar
  • incubation temperature: 28
  • incubation time: 2
  • software version: Sherlock 2.11
  • system: MIS MIDI
  • instrument: HP 6890
  • method/protocol: Kämpfer & Kroppenstedt 1996

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcountryorigin.countrycontinentenrichment cultureenrichment culture durationenrichment culture temperatureisolation procedure
42873rhizosphere of field-grown Zea mays plantsZea maysNebraska, DunbarUSAUSANorth America
43173rhizosphere of maize plantsfield in Dunbar, NebraskaUSAUSANorth Americanutrient agar (NA; Sigma-Aldrich)2 days30serial dilution plating technique

isolation source categories

Cat1Cat2Cat3
#Engineered#Agriculture#Field
#Host#Plants#Herbaceous plants (Grass,Crops)
#Host Body-Site#Plant#Rhizosphere

Safety information

risk assessment

  • @ref: 42873
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 42873
  • description: Bacillus zeae strain JJ-247 16S ribosomal RNA gene, partial sequence
  • accession: KY114944
  • length: 1473
  • database: ena
  • NCBI tax ID: 1917180

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Mesobacillus zeae JJ-247GCA_013618945contigncbi1917180
66792Mesobacillus zeae JJ-247GCA_003570705contigncbi1917180
66792Mesobacillus zeae strain DSM 1039641917180.7wgspatric1917180
66792Mesobacillus zeae DSM 1039642913584337draftimg1917180

GC content

@refGC-contentmethod
4317344.5Thermal denaturation, fluorometry
4287344.5fluorimetric

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes71no
motileyes76.397yes
gram-positiveyes89.62no
anaerobicno96.568yes
aerobicyes83.227yes
halophileno77.321no
spore-formingyes91.933yes
glucose-utilyes89.663yes
flagellatedyes56.834yes
thermophileno95.131no
glucose-fermentno94.052no

External links

@ref: 42873

culture collection no.: DSM 103964, CCM 8726, LMG 29876

straininfo link

  • @ref: 96572
  • straininfo: 403779

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny28086067Bacillus zeae sp. nov., isolated from the rhizosphere of Zea mays.Kampfer P, Busse HJ, McInroy JA, Hu CH, Kloepper JW, Glaeser SPInt J Syst Evol Microbiol10.1099/ijsem.0.0017912017Bacillus/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nebraska, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, *Rhizosphere, Sequence Analysis, DNA, *Soil Microbiology, Spermidine/chemistry, Vitamin K 2/analogs & derivatives/chemistry, Zea mays/*microbiologyTranscriptome
Phylogeny33270002Mesobacillus harenae sp. nov., isolated from the sandy soil of a cold desert.Zhang G, Yang R, Chen T, Zhang B, Yang H, Wu X, Gao H, Zhang W, Liu GInt J Syst Evol Microbiol10.1099/ijsem.0.0045942020Bacillaceae/*classification/isolation & purification, Bacterial Typing Techniques, Base Composition, Cold Temperature, DNA, Bacterial/genetics, *Desert Climate, Fatty Acids/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sand/*microbiology, Sequence Analysis, DNA, *Soil Microbiology, Tibet, Vitamin K 2/analogs & derivatives/chemistryTranscriptome

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
42873Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-103964Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 103964)
43173Peter Kämpfer, Hans-Jürgen Busse, John A. McInroy, Chia-Hui Hu, Joseph W. Kloepper, Stefanie P. GlaeserBacillus zeae sp. nov., isolated from the rhizosphere of Zea mays10.1099/ijsem.0.001791IJSEM 67: 1241-1246 201728086067
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar SchomburgBRENDA, the ELIXIR core data resource in 2021: new developments and updates10.1093/nar/gkaa1025Nucleic Acids Res. 49: D498-D508 202033211880
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
96572Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID403779.1