Strain identifier

BacDive ID: 140257

Type strain: Yes

Species: Fusobacterium gastrosuis

Strain Designation: CDW1, P6 Po

Strain history: <- C. De Witte, Univ. Ghent, Belgium; CDW1

NCBI tax ID(s): 1755100 (species)

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General

@ref: 42864

BacDive-ID: 140257

DSM-Number: 101753

keywords: 16S sequence, Bacteria, mesophilic

description: Fusobacterium gastrosuis CDW1 is a mesophilic bacterium that was isolated from pars oesophagea of the stomach of a 6-months old pig.

NCBI tax id

  • NCBI tax id: 1755100
  • Matching level: species

strain history

  • @ref: 42864
  • history: <- C. De Witte, Univ. Ghent, Belgium; CDW1

doi: 10.13145/bacdive140257.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/fusobacteriota
  • domain: Bacteria
  • phylum: Fusobacteriota
  • class: Fusobacteriia
  • order: Fusobacteriales
  • family: Fusobacteriaceae
  • genus: Fusobacterium
  • species: Fusobacterium gastrosuis
  • full scientific name: Fusobacterium gastrosuis De Witte et al. 2017

@ref: 42864

domain: Bacteria

phylum: Fusobacteria

class: Fusobacteriia

order: Clostridiales

family: Fusobacteriaceae

genus: Fusobacterium

species: Fusobacterium gastrosuis

full scientific name: Fusobacterium gastrosuis De Witte et al. 2017

strain designation: CDW1, P6 Po

type strain: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
42864COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
42864PYG MEDIUM (MODIFIED) (DSMZ Medium 104)yeshttps://mediadive.dsmz.de/medium/104Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water

culture temp

  • @ref: 42864
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6838016199urea-hydrolysis
6838029016arginine-hydrolysis
6838016024D-mannose-fermentation
6838016634raffinose-fermentation
6838029985L-glutamate-degradation
6838017632nitrate-reduction
6838027897tryptophan+energy source

metabolite production

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • production: yes

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: +

enzymes

@refvalueactivityec
68380serine arylamidase-
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase-
68380glycin arylamidase-
68380alanine arylamidase-3.4.11.2
68380tyrosine arylamidase-
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase-3.4.11.1
68380phenylalanine arylamidase-
68380leucyl glycin arylamidase-3.4.11.1
68380proline-arylamidase+3.4.11.5
68380L-arginine arylamidase-
68380alkaline phosphatase+3.1.3.1
68380tryptophan deaminase+4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase-3.2.1.21
68380alpha-glucosidase-3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase-3.2.1.23
68380alpha-galactosidase-3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
42864---------------++-+----------
42864---------------++-+----------

Isolation, sampling and environmental information

isolation

  • @ref: 42864
  • sample type: pars oesophagea of the stomach of a 6-months old pig
  • geographic location: Ghent
  • country: Belgium
  • origin.country: BEL
  • continent: Europe
  • latitude: 51.0543
  • longitude: 3.7174

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Suidae (Pig,Swine)
#Host Body-Site#Gastrointestinal tract#Stomach

Sequence information

16S sequences

  • @ref: 42864
  • description: Fusobacterium gastrosuis partial 16S rRNA gene, type strain CDW1T, isolate P6 Po
  • accession: LN906797
  • length: 1512
  • database: ena
  • NCBI tax ID: 1755100

GC content

  • @ref: 42864
  • GC-content: 28.2
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 42864

culture collection no.: DSM 101753, LMG 29236

straininfo link

  • @ref: 96564
  • straininfo: 402731

literature

  • topic: Phylogeny
  • Pubmed-ID: 27816261
  • title: Detection, isolation and characterization of Fusobacterium gastrosuis sp. nov. colonizing the stomach of pigs.
  • authors: De Witte C, Flahou B, Ducatelle R, Smet A, De Bruyne E, Cnockaert M, Taminiau B, Daube G, Vandamme P, Haesebrouck F
  • journal: Syst Appl Microbiol
  • DOI: 10.1016/j.syapm.2016.10.001
  • year: 2016
  • mesh: Anaerobiosis, Animals, Bacterial Typing Techniques, Base Composition, Cluster Analysis, Cytosol/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Fusobacterium/*classification/genetics/*isolation & purification, Fusobacterium Infections/microbiology/*veterinary, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Stomach/*microbiology, Swine
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
42864Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-101753Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 101753)
68380Automatically annotated from API rID32A
96564Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID402731.1