Strain identifier

BacDive ID: 140166

Type strain: Yes

Species: Moheibacter stercoris

Strain Designation: 784B1_12E-Caso, 784 B1_12 E-Caso

Strain history: CIP <- 2014, P. Kämpfer, Giessen Univ., Giessen, Germany: strain 784 B1_12 E-Caso

NCBI tax ID(s): 1628251 (species)

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General

@ref: 42773

BacDive-ID: 140166

DSM-Number: 29388

keywords: genome sequence, 16S sequence, Bacteria, aerobe, Gram-negative, rod-shaped, colony-forming

description: Moheibacter stercoris 784B1_12E-Caso is an aerobe, Gram-negative, rod-shaped bacterium that forms circular colonies and was isolated from input substrate for a biogas plant: mixed sample of manure and slurry from fatting pigs, dairy cattle and laying hens.

NCBI tax id

  • NCBI tax id: 1628251
  • Matching level: species

strain history

@refhistory
42773<- P. Kämpfer, Justus-Liebig-Universität, Giessen, Germany; 784 B1_12 E-Caso <- T. Schauss
123316CIP <- 2014, P. Kämpfer, Giessen Univ., Giessen, Germany: strain 784 B1_12 E-Caso

doi: 10.13145/bacdive140166.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Flavobacteriia
  • order: Flavobacteriales
  • family: Weeksellaceae
  • genus: Moheibacter
  • species: Moheibacter stercoris
  • full scientific name: Moheibacter stercoris Schauss et al. 2016

@ref: 42773

domain: Bacteria

phylum: Bacteroidetes

class: Flavobacteriia

order: Flavobacteriales

family: Weeksellaceae

genus: Moheibacter

species: Moheibacter stercoris

full scientific name: Moheibacter stercoris Schauss et al. 2016

strain designation: 784B1_12E-Caso, 784 B1_12 E-Caso

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
43614negative1.2 µm0.6 µmrod-shapedno
123316negativeoval-shapedno

colony morphology

  • @ref: 43614
  • colony size: 4 mm
  • colony color: Orange
  • colony shape: circular
  • medium used: CASO agar

pigmentation

  • @ref: 43614
  • production: yes
  • name: Flexirubin-type

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
43614CASO agaryes
42773CASO AGAR (MERCK 105458) (DSMZ Medium 220)yeshttps://mediadive.dsmz.de/medium/220Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
123316CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
43614positivegrowth15-36
42773positivegrowth36mesophilic

culture pH

  • @ref: 43614
  • ability: positive
  • type: growth
  • pH: 6.5-12.5
  • PH range: alkaliphile

Physiology and metabolism

oxygen tolerance

  • @ref: 43614
  • oxygen tolerance: aerobe

halophily

  • @ref: 43614
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 1-4 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4361417128adipate-assimilation
4361417234glucose-assimilation
4361417306maltose-assimilation
4361437684mannose-assimilation
43614506227N-acetylglucosamine-assimilation
4361418401phenylacetate-assimilation
4361417057cellobiose-builds acid from
4361417108D-arabinose-builds acid from
4361428087glycogen-builds acid from
4361462345L-rhamnose-builds acid from
4361417306maltose-builds acid from
43614potassium 5-dehydro-D-gluconate-builds acid from
4361428017starch-builds acid from

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitiveis resistant
436142676amoxicillinyesyes
436147809oxacillinyesyes
4361427902tetracyclineyesyes
4361418186tyrosineyesyes
4361445924trimethoprimyesyes
436149332sulfamethoxazoleyesyes

metabolite production

  • @ref: 43614
  • Chebi-ID: 35581
  • metabolite: indole
  • production: yes

enzymes

@refvalueactivityec
43614cytochrome oxidase+1.9.3.1
43614gelatinase+
43614alkaline phosphatase+3.1.3.1
43614leucine arylamidase+3.4.11.1
43614valine arylamidase+
43614cystine arylamidase+3.4.11.3
43614trypsin+3.4.21.4
43614alpha-chymotrypsin+3.4.21.1
43614acid phosphatase+3.1.3.2
43614naphthol-AS-BI-phosphohydrolase+
43614N-acetyl-beta-glucosaminidase+3.2.1.52
43614esterase (C 4)-
43614esterase Lipase (C 8)-
43614lipase (C 14)-
43614alpha-galactosidase-3.2.1.22
43614beta-galactosidase-3.2.1.23
43614beta-glucuronidase-3.2.1.31
43614alpha-glucosidase-3.2.1.20
43614beta-glucosidase-3.2.1.21
43614alpha-mannosidase-3.2.1.24
43614alpha-fucosidase-3.2.1.51

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    43614C15:0 anteiso0.6
    43614anteiso-C15:2A0.4
    43614C17:0 anteiso0.1
    43614C10:00.3
    43614C14:00.9
    43614C14:0 2OH0.2
    43614C15:0 2OH0.4
    43614C16:04.3
    43614C16:0 2OH0.3
    43614C16:0 3OH2
    43614C16:1ω7c / C16:1ω6c14.4
    43614C17:0 2OH0.3
    43614C17:0 3OH0.4
    43614C18:00.1
    43614C10:0 iso0.2
    43614C13:0 iso0.1
    43614C14:0 iso 3OH0.3
    43614C15:0 iso35
    43614C15:0 iso 3OH2.8
    43614C15:1 iso G24.9
    43614C16:0 iso 3OH0.1
    43614C17:0 iso0.1
    43614C17:0 iso 3OH12.6
  • type of FA analysis: whole cell analysis
  • incubation medium: TSA
  • agar/liquid: agar
  • incubation temperature: 28
  • incubation time: 3
  • incubation_oxygen: aerobic
  • software version: Sherlock 2.1
  • library/peak naming table: TSBA4.1
  • system: MIS MIDI
  • cutoff value:

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
42773input substrate for a biogas plant: mixed sample of manure and slurry from fatting pigs, dairy cattle and laying hensHessenGermanyDEUEurope
43614an organic waste sample composed of manure and slurry from fatting pigs, dairy cattle, and laying hens mixed in a ratio of 10:7 which was applied as input material to a German biogas plant2012
123316Biogas plant input sampleHessenGermanyDEUEurope2012-01-02

isolation source categories

Cat1Cat2Cat3
#Engineered#Industrial#Plant (Factory)
#Engineered#Waste#Solid waste
#Host#Mammals#Bovinae (Cow, Cattle)
#Host#Mammals#Suidae (Pig,Swine)
#Host Body Product#Gastrointestinal tract#Feces (Stool)

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
427731Risk group (German classification)
1233161Risk group (French classification)

Sequence information

16S sequences

  • @ref: 42773
  • description: Moheibacter stercoris strain 784B1_12E-Caso 16S ribosomal RNA gene, partial sequence
  • accession: KP119861
  • length: 1438
  • database: ena
  • NCBI tax ID: 1628251

Genome sequences

  • @ref: 66792
  • description: Moheibacter stercoris DSM 29388
  • accession: 2928575895
  • assembly level: draft
  • database: img
  • NCBI tax ID: 1628251

GC content

@refGC-contentmethod
4361434.5thermal denaturation, midpoint method (Tm)
4277334.5fluorimetric

External links

@ref: 42773

culture collection no.: DSM 29388, CIP 110830, LMG 28502

straininfo link

  • @ref: 96543
  • straininfo: 401879

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny27088161Moheibacter stercoris sp. nov., isolated from an input sample of a biogas plant.Schauss T, Busse HJ, Golke J, Kampfer P, Glaeser SPInt J Syst Evol Microbiol10.1099/ijsem.0.0010892016Bacterial Typing Techniques, *Biofuels, DNA, Bacterial/genetics, Fatty Acids/chemistry, Flavobacteriaceae/*classification/genetics/isolation & purification, Germany, Manure/*microbiology, Phosphatidylethanolamines/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Spermidine/analogs & derivatives/chemistry, Vitamin K 2/analogs & derivatives/chemistryTranscriptome
Phylogeny33813642Moheibacter lacus sp. nov., Isolated from Freshwater Lake Sediment.Liu Y, Chang YQ, Wang CN, Ye MQ, Wang MY, Du ZJCurr Microbiol10.1007/s00284-021-02465-12021Bacterial Typing Techniques, China, DNA, Bacterial/genetics, Fatty Acids, *Flavobacteriaceae/genetics, Geologic Sediments, *Lakes, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2Transcriptome

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
42773Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-29388Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 29388)
43614Thorsten Schauss, Hans-Jürgen Busse, Jan Golke, Peter Kämpfer, Stefanie P. GlaeserMoheibacter stercoris sp. nov., isolated from an input sample of a biogas plant10.1099/ijsem.0.001089IJSEM 66: 2585-2591 201627088161
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
96543Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID401879.1
123316Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20110830Collection of Institut Pasteur (CIP 110830)