Strain identifier
BacDive ID: 140089
Type strain: 
Species: Thermoanaerobacterium butyriciformans
Strain Designation: USBA-19
Strain history: <- M. Rey Arevalo, Pontificia Univ. Javeriana, Biologia and Bicrobiologia, Bogotá, Colombia; USBA-19 <- S. Baena {2002}
NCBI tax ID(s): 1702242 (species)
version 8.1 (current version)
General
@ref: 42696
BacDive-ID: 140089
DSM-Number: 101588
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, spore-forming, thermophilic, motile
description: Thermoanaerobacterium butyriciformans USBA-19 is an anaerobe, spore-forming, thermophilic bacterium that was isolated from thermal spring P3, 2500 m altitude.
NCBI tax id
- NCBI tax id: 1702242
- Matching level: species
strain history
- @ref: 42696
- history: <- M. Rey Arevalo, Pontificia Univ. Javeriana, Biologia and Bicrobiologia, Bogotá, Colombia; USBA-19 <- S. Baena {2002}
doi: 10.13145/bacdive140089.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Clostridia
- order: Thermoanaerobacterales
- family: Thermoanaerobacteraceae
- genus: Thermoanaerobacterium
- species: Thermoanaerobacterium butyriciformans
- full scientific name: Thermoanaerobacterium butyriciformans López et al. 2019
@ref: 42696
domain: Bacteria
phylum: Firmicutes
class: Clostridia
order: Clostridiales
family: Thermoanaerobacteraceae
genus: Thermoanaerobacterium
species: Thermoanaerobacterium butyriciformans
full scientific name: Thermoanaerobacterium butyriciformans López et al. 2019
strain designation: USBA-19
type strain: yes
Morphology
cell morphology
| @ref | motility | confidence | gram stain |
|---|---|---|---|
| 69480 | yes | 90.688 | |
| 69480 | 100 | positive |
Culture and growth conditions
culture medium
- @ref: 42696
- name: CALDICELLULOSIRUPTOR MEDIUM (DSMZ Medium 640)
- growth: yes
- link: https://mediadive.dsmz.de/medium/640
- composition: Name: CALDICELLULOSIRUPTOR MEDIUM (DSMZ Medium 640; with strain-specific modifications) Composition: D-Glucose 5.0 g/l Trypticase peptone 2.0 g/l K2HPO4 1.5 g/l Yeast extract 1.0 g/l NH4Cl 0.9 g/l NaCl 0.9 g/l KH2PO4 0.75 g/l L-Cysteine HCl x H2O 0.75 g/l MgCl2 x 6 H2O 0.4 g/l HCl 0.0025 g/l FeCl3 x 6 H2O 0.0025 g/l FeCl2 x 4 H2O 0.0015 g/l Sodium resazurin 0.0005 g/l CoCl2 x 6 H2O 0.00019 g/l MnCl2 x 4 H2O 0.0001 g/l ZnCl2 7e-05 g/l Na2MoO4 x 2 H2O 3.6e-05 g/l NiCl2 x 6 H2O 2.4e-05 g/l H3BO3 6e-06 g/l CuCl2 x 2 H2O 2e-06 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range | confidence |
|---|---|---|---|---|---|
| 42696 | positive | growth | 55 | thermophilic | |
| 69480 | thermophilic | 99.152 |
Physiology and metabolism
oxygen tolerance
- @ref: 69480
- oxygen tolerance: anaerobe
- confidence: 100
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 69481 | yes | 98 |
| 69480 | yes | 100 |
Isolation, sampling and environmental information
isolation
- @ref: 42696
- sample type: thermal spring P3, 2500 m altitude
- geographic location: Ojo del Diablo, Paipa
- country: Colombia
- origin.country: COL
- continent: Middle and South America
- latitude: 5.7593
- longitude: -73.1139
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Condition | #Thermophilic (>45°C) | |
| #Environmental | #Aquatic | #Thermal spring |
Sequence information
16S sequences
- @ref: 42696
- description: Thermoanaerobacterium sp. USBA-019 16S ribosomal RNA gene, partial sequence
- accession: KT369738
- length: 1335
- database: ena
- NCBI tax ID: 1702242
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Thermoanaerobacterium butyriciformans DSM 101588 | GCA_017874545 | contig | ncbi | 1702242 |
| 66792 | Thermoanaerobacterium butyriciformans strain DSM 101588 | 1702242.3 | wgs | patric | 1702242 |
| 66792 | Thermoanaerobacterium butyriciformans DSM 101588 | 2913561130 | draft | img | 1702242 |
GC content
- @ref: 42696
- GC-content: 32.7
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | yes | 98 | no |
| motile | yes | 86.467 | no |
| gram-positive | no | 73.225 | no |
| anaerobic | yes | 99.271 | no |
| aerobic | no | 99.085 | no |
| halophile | no | 96.284 | no |
| spore-forming | yes | 96.201 | no |
| thermophile | yes | 99.724 | yes |
| glucose-util | yes | 90.054 | no |
| flagellated | no | 75.894 | no |
| glucose-ferment | yes | 56.631 | no |
External links
@ref: 42696
culture collection no.: DSM 101588, CMPUJ U-019
straininfo link
- @ref: 96501
- straininfo: 401764
literature
- topic: Phylogeny
- Pubmed-ID: 28057375
- title: Description of a new anaerobic thermophilic bacterium, Thermoanaerobacterium butyriciformans sp. nov.
- authors: Lopez G, Canas-Duarte SJ, Pinzon-Velasco AM, Vega-Vela NE, Rodriguez M, Restrepo S, Baena S
- journal: Syst Appl Microbiol
- DOI: 10.1016/j.syapm.2016.11.006
- year: 2016
- mesh: Alcohols/metabolism, Anaerobiosis, Bacterial Typing Techniques, Carbohydrate Metabolism, Carboxylic Acids/metabolism, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fermentation, Hot Springs/*microbiology, Hydrogen-Ion Concentration, Nitrates/metabolism, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Spores, Bacterial/cytology, Sulfur/metabolism, Temperature, Thermoanaerobacterium/*classification/genetics/*isolation & purification, Thiosulfates/metabolism
- topic2: Metabolism
Reference
| @id | authors | title | doi/url | catalogue |
|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
| 42696 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-101588 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 101588) | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | Predictions based on the model GenomeNet Sporulation v. 1 | 10.21203/rs.3.rs-2527258/v1 | |
| 96501 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID401764.1 |