General

@ref: 42651

BacDive-ID: 140044

DSM-Number: 25616

keywords: Archaea, mesophilic

description: Methanobacterium lacus AL21 is a mesophilic archaeon that was isolated from peat soil from an acidic poor fen.

NCBI tax id

• NCBI tax id: 877455
• Matching level: species

strain history

• @ref: 42651
• history: <- H. Cadillo-Quiroz, School of Life Sciences, Arizona State University, USA (Methanobacterium boreale) <- H. Cadillo-Quiroz, Dept. Microbiology, Cornell Univ., Ithaca, USA {2008}

doi: 10.13145/bacdive140044.20230509.8.1

Name and taxonomic classification

LPSN

• @ref: 20215
• description: domain/archaea
• keyword: phylum/methanobacteriota
• domain: Archaea
• phylum: Methanobacteriota
• class: Methanobacteria
• order: Methanobacteriales
• family: Methanobacteriaceae
• genus: Methanobacterium
• species: Methanobacterium lacus
• full scientific name: Methanobacterium lacus Borrel et al. 2012

@ref: 42651

domain: Archaea

phylum: Euryarchaeota

class: Methanobacteria

order: Methanobacteriales

family: Methanobacteriaceae

genus: Methanobacterium

species: Methanobacterium lacus

full scientific name: Methanobacterium lacus Borrel et al. 2012

strain designation: AL21

type strain: no

Culture and growth conditions

culture medium

• @ref: 42651
• name: METHANOSPHAERULA (PEAT) MEDIUM (DSMZ Medium 1094)
• growth: yes
• link: https://mediadive.dsmz.de/medium/1094
• composition: Name: METHANOSPHAERULA (PEAT) MEDIUM (DSMZ Medium 1094; with strain-specific modifications) Composition: 2-(N-Morpholino)ethanesulfonic acid 1.93644 g/l Na-acetate 0.40715 g/l NaOH 0.19861 g/l TiCl3 0.0819265 g/l 2-Mercaptoethanesulfonic acid 0.0794439 g/l Na2-EDTA 0.0369712 g/l NH4Cl 0.0266137 g/l Yeast extract 0.019861 g/l KH2PO4 0.0135055 g/l Na2S x 9 H2O 0.00953327 g/l AlK(SO4)2 x 12 H2O 0.00342602 g/l CaCl2 x 2 H2O 0.00232373 g/l MgSO4 x 7 H2O 0.00154916 g/l KCl 0.00148957 g/l FeCl2 x 4 H2O 0.00133069 g/l Pyridoxine hydrochloride 9.93049e-05 g/l ZnCl2 7.44787e-05 g/l p-Aminobenzoic acid 4.96524e-05 g/l Nicotinic acid 4.96524e-05 g/l Riboflavin 4.96524e-05 g/l Thiamine HCl 4.96524e-05 g/l (DL)-alpha-Lipoic acid 4.96524e-05 g/l Calcium D-(+)-pantothenate 4.96524e-05 g/l MnSO4 x 4 H2O 2.58193e-05 g/l CoCl2 x 6 H2O 2.38332e-05 g/l NiCl2 x 6 H2O 2.38332e-05 g/l Na2Mo4 x 2 H2O 2.38332e-05 g/l Folic acid 1.9861e-05 g/l Biotin 1.9861e-05 g/l H3BO3 1.88679e-05 g/l CuSO4 x 5 H2O 8.93744e-06 g/l Vitamin B12 9.93049e-07 g/l Na3-NTA x H2O Tris-HCl buffer Distilled water

culture temp

• @ref: 42651
• growth: positive
• type: growth
• temperature: 28
• range: mesophilic

Isolation, sampling and environmental information

isolation

• @ref: 42651
• sample type: peat soil from an acidic poor fen
• geographic location: Alaska, Baxter fen near Anchorage
• country: USA
• origin.country: USA
• continent: North America
• latitude: 61.5333
• longitude: -150.45

isolation source categories

Safety information

risk assessment

• @ref: 42651
• biosafety level: 1
• biosafety level comment: Risk group (German classification)

Sequence information

GC content

• @ref: 42651
• GC-content: 35.8
• method: sequence analysis

External links

@ref: 42651

culture collection no.: DSM 25616

straininfo link

• @ref: 96464
• straininfo: 405963

Reference