Strain identifier

BacDive ID: 139988

Type strain: Yes

Species: Peribacillus asahii

Strain Designation: MA001

Strain history: CIP <- 2005, JCM <- 2003, K. Takizawa: strain MA001 <- A. Kasei Co Ltd

NCBI tax ID(s): 228899 (species)

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General

@ref: 42596

BacDive-ID: 139988

DSM-Number: 105779

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, spore-forming, mesophilic, Gram-positive, motile, rod-shaped

description: Peribacillus asahii MA001 is an obligate aerobe, spore-forming, mesophilic bacterium that was isolated from soil.

NCBI tax id

  • NCBI tax id: 228899
  • Matching level: species

strain history

@refhistory
42596<- JCM, RIKEN BioResource Center, Tsukuba; JCM 12112 <- K. Takizawa, Asahi Vet Japan Co., Ltd, Hokkaido, Japan; MA001
67770K. Takizawa MA001 <-- Asahi Kasei Co., Ltd..
116520CIP <- 2005, JCM <- 2003, K. Takizawa: strain MA001 <- A. Kasei Co Ltd

doi: 10.13145/bacdive139988.20250331.9.3

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Peribacillus
  • species: Peribacillus asahii
  • full scientific name: Peribacillus asahii (Yumoto et al. 2004) Patel and Gupta 2020

  • synonyms

    • @ref: 20215
    • synonym: Bacillus asahii

@ref: 42596

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Peribacillus

species: Peribacillus asahii

full scientific name: Peribacillus asahii (Yumoto et al. 2004) Patel and Gupta 2020

strain designation: MA001

type strain: yes

Morphology

cell morphology

  • @ref: 116520
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 116520

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
42596CASO AGAR (MERCK 105458) (DSMZ Medium 220)yeshttps://mediadive.dsmz.de/medium/220Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
40044MEDIUM 469 - for Pseudomonas psychrophilayesDistilled water make up to (1000.000 ml);Sodium chloride (5.000 g);Agar(20.000 g);Yeast extract (10.000 g);Peptone (10.000 g)
116520CIP Medium 469yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=469

culture temp

@refgrowthtypetemperature
42596positivegrowth30
67770positivegrowth30
116520positivegrowth22-45
116520nogrowth10
116520nogrowth55

culture pH

  • @ref: 116520
  • ability: positive
  • type: growth
  • pH: 6

Physiology and metabolism

oxygen tolerance

  • @ref: 116520
  • oxygen tolerance: obligate aerobe

spore formation

@refspore formationconfidence
125438yes90.298
116520yes

halophily

@refsaltgrowthtested relationconcentration
116520NaClnogrowth0 %
116520NaClnogrowth2 %
116520NaClnogrowth4 %
116520NaClnogrowth6 %
116520NaClnogrowth8 %
116520NaClnogrowth10 %

observation

  • @ref: 67770
  • observation: Deodorizes bad smell

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
11652016947citrate-carbon source
1165204853esculin-hydrolysis
116520606565hippurate+hydrolysis
11652017632nitrate-reduction
11652016301nitrite-reduction
11652017632nitrate-respiration

metabolite production

  • @ref: 116520
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
11652015688acetoin-
11652017234glucose-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin+3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
116520oxidase+
116520beta-galactosidase+3.2.1.23
116520alcohol dehydrogenase-1.1.1.1
116520gelatinase+/-
116520amylase+
116520DNase+
116520caseinase-3.4.21.50
116520catalase+1.11.1.6
116520tween esterase-
116520gamma-glutamyltransferase+2.3.2.2
116520lecithinase-
116520lipase-
116520lysine decarboxylase-4.1.1.18
116520ornithine decarboxylase-4.1.1.17
116520urease+3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
116520--+--+---+-+---+----

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
116520-------------------------------------------------+---------------++------+-+---+++-+----+++--+---++

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
42596soilTagata-gun, ShizuokaJapanJPNAsia
67770Soil obtained from Tagata-gunShizuokaJapanJPNAsia
116520Environment, SoilShizuokaJapanJPNAsia

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_22110.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_370;97_418;98_12241;99_22110&stattab=map
  • Last taxonomy: Peribacillus
  • 16S sequence: AB109209
  • Sequence Identity:
  • Total samples: 18866
  • soil counts: 3392
  • aquatic counts: 790
  • animal counts: 14335
  • plant counts: 349

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
425961Risk group (German classification)
1165201Risk group (French classification)

Sequence information

16S sequences

  • @ref: 42596
  • description: Bacillus asahii gene for 16S rRNA, partial sequence
  • accession: AB109209
  • length: 1504
  • database: nuccore
  • NCBI tax ID: 228899

Genome sequences

  • @ref: 67770
  • description: Peribacillus asahii MA001
  • accession: GCA_003570725
  • assembly level: contig
  • database: ncbi
  • NCBI tax ID: 228899

GC content

  • @ref: 67770
  • GC-content: 39.4
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

@refmodeltraitdescriptionpredictionconfidencetraining_data
125438gram-positivegram-positivePositive reaction to Gram-stainingyes77.084no
125438anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no96.865yes
125438aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)yes79.042yes
125438spore-formingspore-formingAbility to form endo- or exosporesyes90.298no
125438thermophilethermophilicAbility to grow at temperatures above or equal to 45°Cno91.288yes
125438motile2+flagellatedAbility to perform flagellated movementyes81.43no

External links

@ref: 42596

culture collection no.: DSM 105779, JCM 12112, NCIMB 13969, CIP 108638, FERM BP-4493

straininfo link

  • @ref: 96419
  • straininfo: 134414

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny15545424Bacillus asahii sp. nov., a novel bacterium isolated from soil with the ability to deodorize the bad smell generated from short-chain fatty acids.Yumoto I, Hirota K, Yamaga S, Nodasaka Y, Kawasaki T, Matsuyama H, Nakajima KInt J Syst Evol Microbiol10.1099/ijs.0.03014-02004Amino Acid Oxidoreductases/analysis, Bacillus/*classification/cytology/*isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, Carbohydrate Metabolism, Carboxylic Acids/metabolism, Catalase/analysis, DNA, Bacterial/chemistry/isolation & purification, DNA, Ribosomal/chemistry/isolation & purification, Fatty Acids/analysis/isolation & purification, Fatty Acids, Volatile/*metabolism, Flagella, Genes, rRNA, Gentian Violet, Hydrogen Sulfide/metabolism, Hydrogen-Ion Concentration, Indoles/metabolism, Japan, Molecular Sequence Data, Nucleic Acid Hybridization, *Odorants, Oxidoreductases/analysis, Phenazines, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Saline Solution, Hypertonic, Sequence Analysis, DNA, *Soil Microbiology, Spores, Bacterial/cytologyEnzymology
Phylogeny24903955Bacillus huizhouensis sp. nov., isolated from a paddy field soil.Li J, Yang G, Wu M, Zhao Y, Zhou SAntonie Van Leeuwenhoek10.1007/s10482-014-0208-22014Aerobiosis, Anaerobiosis, Bacillus/*classification/*isolation & purification, Bacterial Typing Techniques, Base Composition, Cluster Analysis, Cytosol/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Hydrogen-Ion Concentration, Locomotion, Molecular Sequence Data, Oryza, Phospholipids/analysis, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride/metabolism, *Soil Microbiology, Spores, Bacterial/cytology, Temperature, Vitamin K 2/analysisGenetics
Phylogeny26612584Bacillus cavernae sp. nov. isolated from cave soil.Feng L, Liu D, Sun X, Wang G, Li MInt J Syst Evol Microbiol10.1099/ijsem.0.0007942015
Phylogeny30063204Bacillus acanthi sp. nov., isolated from the rhizosphere soil of a mangrove plant Acanthus ilicifolius.Ma K, Yin Q, Chen L, Lai Q, Xu YInt J Syst Evol Microbiol10.1099/ijsem.0.0029502018Acanthaceae/*microbiology, Bacillus/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Hong Kong, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, *Rhizosphere, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistryTranscriptome

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
40044Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/6289
42596Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-105779Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 105779)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar SchomburgBRENDA, the ELIXIR core data resource in 2021: new developments and updates10.1093/nar/gkaa1025Nucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
96419Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID134414.1
116520Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108638Collection of Institut Pasteur (CIP 108638)
125438Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg OvermannPredicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets10.1101/2024.08.12.607695