Archive BacDiveID:10.13145/bacdive139921.20250331.9.3

Strain identifier

BacDive ID: 139921

Type strain:

Species: Bosea sp.

Strain Designation: WAO

Strain history: <- A. Walczak, Rutgers, State Univ. New Jersey, New Brunswick, USA; WAO <- D. Rhine

NCBI tax ID(s): 406341 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.

General

@ref: 42529

BacDive-ID: 139921

DSM-Number: 102914

keywords: genome sequence, 16S sequence, Bacteria, mesophilic, Gram-negative

description: Bosea sp. WAO is a mesophilic, Gram-negative bacterium that was isolated from Aerobic black shale slurry amended with 5mM As.

NCBI tax id

NCBI tax id: 406341
Matching level: species

strain history

@ref: 42529
history: <- A. Walczak, Rutgers, State Univ. New Jersey, New Brunswick, USA; WAO <- D. Rhine

doi: 10.13145/bacdive139921.20250331.9.3

Name and taxonomic classification

LPSN

@ref: 20215
description: domain/bacteria
keyword: phylum/pseudomonadota
domain: Bacteria
phylum: Pseudomonadota
class: Alphaproteobacteria
order: Hyphomicrobiales
family: Boseaceae
genus: Bosea
species: Bosea sp.
full scientific name: Bosea Das et al. 1996

@ref: 42529

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhizobiales

family: Boseaceae

genus: Bosea

species: Bosea sp.

full scientific name: Bosea sp.

strain designation: WAO

type strain: no

Morphology

cell morphology

@ref	gram stain	confidence
125438	negative	97.833
125439	negative	96.6

Culture and growth conditions

culture medium

@ref	name	growth	link	composition
42529	TRYPTONE SOYA BROTH (TSB) (DSMZ Medium 545)	yes	https://mediadive.dsmz.de/medium/545	Name: TRYPTONE SOYA BROTH (TSB) (DSMZ Medium 545) Composition: Casein peptone 17.0 g/l NaCl 5.0 g/l Soy peptone 3.0 g/l D(+)-Glucose 2.5 g/l K2HPO4 2.5 g/l Distilled water
42529	TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)	yes	https://mediadive.dsmz.de/medium/535	Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water

culture temp

@ref: 42529
growth: positive
type: growth
temperature: 28

Physiology and metabolism

oxygen tolerance

@ref: 125439
oxygen tolerance: aerobe
confidence: 95.1

spore formation

@ref: 125439
spore formation: no
confidence: 96.8

Isolation, sampling and environmental information

isolation

@ref: 42529
sample type: Aerobic black shale slurry amended with 5mM As(III)
country: USA
origin.country: USA
continent: North America
latitude: 40.2893
longitude: 74.8144

isolation source categories

Cat1: #Environmental
Cat2: #Terrestrial
Cat3: #Mud (Sludge)

Sequence information

16S sequences

@ref: 42529
description: Bosea sp. WAO 16S ribosomal RNA gene, partial sequence
accession: DQ986321.1
length: 1207
database: nuccore
NCBI tax ID: 406341

Genome sequences

@ref	description	accession	assembly level	database	NCBI tax ID
66792	Bosea sp. WAO	GCA_001509835	contig	ncbi	406341
66792	Bosea sp. DSM 102914	2735892.3	wgs	patric	406341
66792	Bosea sp. WAO	406341.6	wgs	patric	406341
66792	Bosea sp. DSM 102914	2861304655	draft	img	2735892

Genome-based predictions

predictions

@ref	model	trait	description	prediction	confidence	training_data
125438	gram-positive	gram-positive	Positive reaction to Gram-staining	no	97.833	no
125438	anaerobic	anaerobic	Ability to grow under anoxygenic conditions (including facultative anaerobes)	no	96.501	no
125438	spore-forming	spore-forming	Ability to form endo- or exospores	no	89.319	no
125438	thermophile	thermophilic	Ability to grow at temperatures above or equal to 45°C	no	98.332	yes
125438	aerobic	aerobic	Ability to grow under oxygenic conditions (including facultative aerobes)	yes	83.2	no
125438	motile2+	flagellated	Ability to perform flagellated movement	yes	73.031	no
125439	BacteriaNet	spore_formation	Ability to form endo- or exospores	no	96.8
125439	BacteriaNet	motility	Ability to perform movement	yes	70.5
125439	BacteriaNet	gram_stain	Reaction to gram-staining	negative	96.6
125439	BacteriaNet	oxygen_tolerance	Oxygenic conditions needed for growth	aerobe	95.1

External links

@ref: 42529

culture collection no.: DSM 102914

straininfo link

@ref: 96351
straininfo: 400091

literature

topic: Genetics
Pubmed-ID: 29682167
title: Draft genome sequence of Bosea sp. WAO an arsenite and sulfide oxidizer isolated from a pyrite rock outcrop in New Jersey.
authors: Walczak AB, Yee N, Young LY
journal: Stand Genomic Sci
DOI: 10.1186/s40793-018-0312-4
year: 2018

Reference

@id	authors	title	doi/url	catalogue
20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.	List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ	10.1099/ijsem.0.004332
42529	Curators of the DSMZ		https://www.dsmz.de/collection/catalogue/details/culture/DSM-102914	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 102914)
66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann	Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)	https://diaspora-project.de/progress.html#genomes
96351	Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.	StrainInfo: A central database for resolving microbial strain identifiers	10.60712/SI-ID400091.1
125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann	Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets	10.1101/2024.08.12.607695
125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy	deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1	https://github.com/GenomeNet/deepG