Strain identifier
BacDive ID: 139869
Type strain: 
Species: Vibrio cholerae
Strain Designation: A1552
Strain history: <- F. Kemter, Univ. Marburg <- T. Waldminghaus, Univ. Marburg <- M. Blokesch, Ecole Polytechnique Fédérale de Lausanne, Switzerland <- G. Schoolnik and F.H. Yildiz, Stanford University; A1552
NCBI tax ID(s): 666 (species)
version 7.1 (current version)
General
@ref: 42477
BacDive-ID: 139869
DSM-Number: 106276
keywords: genome sequence, Bacteria, mesophilic
description: Vibrio cholerae A1552 is a mesophilic bacterium that was isolated from traveler from Peru.
NCBI tax id
- NCBI tax id: 666
- Matching level: species
strain history: <- F. Kemter, Univ. Marburg <- T. Waldminghaus, Univ. Marburg <- M. Blokesch, Ecole Polytechnique Fédérale de Lausanne, Switzerland <- G. Schoolnik and F.H. Yildiz, Stanford University; A1552
doi: 10.13145/bacdive139869.20221219.7.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Vibrionales
- family: Vibrionaceae
- genus: Vibrio
- species: Vibrio cholerae
- full scientific name: Vibrio cholerae Pacini 1854 (Approved Lists 1980)
synonyms
- @ref: 20215
- synonym: Vibrio albensis
@ref: 42477
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Vibrionales, not validated
family: Vibrionaceae
genus: Vibrio
species: Vibrio cholerae
full scientific name: Vibrio cholerae Pacini 1854
strain designation: A1552
type strain: no
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 42477 | COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) | yes | https://mediadive.dsmz.de/medium/693 | Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base |
| 42477 | TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) | yes | https://mediadive.dsmz.de/medium/92 | Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water |
culture temp
- @ref: 42477
- growth: positive
- type: growth
- temperature: 37
- range: mesophilic
Physiology and metabolism
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 68368 | 30849 | L-arabinose | - | fermentation |
| 68368 | 27613 | amygdalin | - | fermentation |
| 68368 | 28053 | melibiose | - | fermentation |
| 68368 | 17992 | sucrose | + | fermentation |
| 68368 | 62345 | L-rhamnose | - | fermentation |
| 68368 | 30911 | sorbitol | - | fermentation |
| 68368 | 17268 | myo-inositol | - | fermentation |
| 68368 | 27897 | tryptophan | + | energy source |
| 68368 | 16947 | citrate | + | assimilation |
| 68368 | 18257 | ornithine | + | degradation |
| 68368 | 29016 | arginine | - | hydrolysis |
metabolite production
| @ref | Chebi-ID | metabolite | production |
|---|---|---|---|
| 68368 | 35581 | indole | yes |
| 68368 | 16136 | hydrogen sulfide | no |
metabolite tests
- @ref: 68368
- Chebi-ID: 35581
- metabolite: indole
- indole test: +
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 68368 | cytochrome oxidase | + | 1.9.3.1 |
| 68368 | tryptophan deaminase | - | 4.1.99.1 |
| 68368 | ornithine decarboxylase | + | 4.1.1.17 |
| 68368 | arginine dihydrolase | - | 3.5.3.6 |
| 68368 | beta-galactosidase | + | 3.2.1.23 |
API 20E
| @ref | ONPG | ADH Arg | LDC Lys | ODC | CIT | H2S | URE | TDA Trp | IND | VP | GEL | GLU | MAN | INO | Sor | RHA | SAC | MEL | AMY | ARA | OX |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 42477 | + | - | - | + | + | - | - | - | + | - | - | +/- | + | - | - | - | + | - | - | - | + |
| 42477 | + | - | + | + | + | - | + | - | + | + | + | +/- | +/- | - | - | - | + | - | - | - | + |
Isolation, sampling and environmental information
isolation
- @ref: 42477
- sample type: traveler from Peru
- country: Peru
- origin.country: PER
- continent: Middle and South America
- latitude: -10
- longitude: -76
Safety information
risk assessment
- @ref: 42477
- biosafety level: 2
- biosafety level comment: Risk group (German classification)
Sequence information
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Vibrio cholerae A1552 | GCA_017948285 | complete | ncbi | 666 |
| 66792 | Vibrio cholerae A1552 | GCA_003097695 | complete | ncbi | 666 |
| 66792 | Vibrio cholerae A1552 | GCA_002997215 | complete | ncbi | 666 |
| 66792 | Vibrio cholerae A1552 | GCA_002892855 | complete | ncbi | 666 |
External links
@ref: 42477
culture collection no.: DSM 106276
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Metabolism | 18681937 | A new Vibrio cholerae sRNA modulates colonization and affects release of outer membrane vesicles. | Song T, Mika F, Lindmark B, Liu Z, Schild S, Bishop A, Zhu J, Camilli A, Johansson J, Vogel J, Wai SN | Mol Microbiol | 10.1111/j.1365-2958.2008.06392.x | 2008 | Animals, Bacterial Outer Membrane Proteins/*genetics, Base Sequence, Cell Membrane/*metabolism, Cholera/microbiology, DNA, Bacterial/genetics, Fimbriae Proteins/genetics, Gene Expression Regulation, Bacterial, Genes, Bacterial, Intestines/microbiology, Mice, Molecular Sequence Data, Protein Biosynthesis, RNA, Bacterial/*genetics, RNA, Untranslated/*genetics, Sequence Deletion, Sigma Factor/genetics, Transcription Initiation Site, Vibrio cholerae O1/*genetics/metabolism | Genetics |
| Metabolism | 19103773 | Role of melanin pigment in expression of Vibrio cholerae virulence factors. | Valeru SP, Rompikuntal PK, Ishikawa T, Vaitkevicius K, Sjoling A, Dolganov N, Zhu J, Schoolnik G, Wai SN | Infect Immun | 10.1128/IAI.00929-08 | 2008 | Bacterial Proteins/genetics, Blotting, Western, DNA Transposable Elements, Gene Expression Regulation, Bacterial/*genetics, Genes, Bacterial/*genetics, Melanins/genetics/*metabolism, Mutation, Oligonucleotide Array Sequence Analysis, Oxidoreductases/*genetics, Reverse Transcriptase Polymerase Chain Reaction, Sigma Factor/genetics, Vibrio cholerae/*genetics/pathogenicity, Virulence Factors/*genetics | Transcriptome |
| Pathogenicity | 19411843 | A novel sRNA that modulates virulence and environmental fitness of Vibrio cholerae. | Song T, Wai SN | RNA Biol | 10.4161/rna.6.3.8371 | 2009 | Animals, Gene Expression Regulation, Bacterial, Genome-Wide Association Study, Humans, Microbial Viability/radiation effects, RNA, Bacterial/*genetics, RNA, Untranslated/*genetics, Vibrio cholerae/*genetics/*pathogenicity/radiation effects, Virulence | Genetics |
| Genetics | 19701456 | Quorum sensing regulation of the two hcp alleles in Vibrio cholerae O1 strains. | Ishikawa T, Rompikuntal PK, Lindmark B, Milton DL, Wai SN | PLoS One | 10.1371/journal.pone.0006734 | 2009 | *Alleles, Amino Acid Sequence, Bacterial Proteins/chemistry/*genetics, Base Sequence, DNA Primers, DNA, Bacterial, *Gene Expression Regulation, Bacterial, *Genes, Bacterial, Hemolysin Proteins/chemistry/*genetics, Molecular Sequence Data, Polymerase Chain Reaction, *Quorum Sensing, Species Specificity, Vibrio cholerae/genetics/*physiology | Phylogeny |
| Metabolism | 21314704 | In situ grazing resistance of Vibrio cholerae in the marine environment. | Erken M, Weitere M, Kjelleberg S, McDougald D | FEMS Microbiol Ecol | 10.1111/j.1574-6941.2011.01067.x | 2011 | *Antibiosis, Bacterial Proteins/genetics/*metabolism, *Biofilms, Frameshift Mutation, Glucose/metabolism, Kinetoplastida/*physiology, Seawater/chemistry/microbiology, Vibrio cholerae/genetics/metabolism/*physiology, Water Microbiology | |
| Metabolism | 22083711 | Pathoadaptive conditional regulation of the type VI secretion system in Vibrio cholerae O1 strains. | Ishikawa T, Sabharwal D, Broms J, Milton DL, Sjostedt A, Uhlin BE, Wai SN | Infect Immun | 10.1128/IAI.05510-11 | 2011 | Bacterial Proteins/genetics/*metabolism, Bacteriological Techniques, Environment, Escherichia coli K12, Gene Expression Regulation, Bacterial/*physiology, Osmolar Concentration, Plasmids, Reverse Transcriptase Polymerase Chain Reaction, Secretory Pathway/*physiology, Temperature, Vibrio cholerae/*classification/*metabolism/pathogenicity, Virulence | Phylogeny |
| Metabolism | 23642157 | A functional VipA-VipB interaction is required for the type VI secretion system activity of Vibrio cholerae O1 strain A1552. | Broms JE, Ishikawa T, Wai SN, Sjostedt A | BMC Microbiol | 10.1186/1471-2180-13-96 | 2013 | Bacterial Proteins/genetics/*metabolism, *Bacterial Secretion Systems, DNA Mutational Analysis, Escherichia coli/growth & development, Humans, Mutant Proteins/genetics/metabolism, *Protein Interaction Mapping, Vibrio cholerae O1/genetics/growth & development/*metabolism | Pathogenicity |
| Metabolism | 24098113 | Role of the Vibrio cholerae matrix protein Bap1 in cross-resistance to antimicrobial peptides. | Duperthuy M, Sjostrom AE, Sabharwal D, Damghani F, Uhlin BE, Wai SN | PLoS Pathog | 10.1371/journal.ppat.1003620 | 2013 | Antimicrobial Cationic Peptides/metabolism/*pharmacology, Bacterial Proteins/genetics/*metabolism, *Drug Resistance, Bacterial, Extracellular Matrix Proteins/genetics/*metabolism, Porins/genetics/*metabolism, Vibrio cholerae/genetics/*metabolism | |
| Genetics | 29496831 | Closed Genome Sequence of Vibrio cholerae O1 El Tor Inaba Strain A1552. | Allue-Guardia A, Echazarreta M, Koenig SSK, Klose KE, Eppinger M | Genome Announc | 10.1128/genomeA.00098-18 | 2018 | ||
| Genetics | 30574591 | Long-Read-Based Genome Sequences of Pandemic and Environmental Vibrio cholerae Strains. | Matthey N, Drebes Dorr NC, Blokesch M | Microbiol Resour Announc | 10.1128/MRA.01574-18 | 2018 | ||
| Metabolism | 32592280 | Elevated levels of VCA0117 (VasH) in response to external signals activate the type VI secretion system of Vibrio cholerae O1 El Tor A1552. | Seibt H, Aung KM, Ishikawa T, Sjostrom A, Gullberg M, Atkinson GC, Wai SN, Shingler V | Environ Microbiol | 10.1111/1462-2920.15141 | 2020 | Bacterial Proteins/metabolism, Gene Expression Regulation, Bacterial/*genetics, Multigene Family/genetics, Promoter Regions, Genetic/genetics, Signal Transduction/physiology, Transcriptional Activation/*genetics, Type VI Secretion Systems/*metabolism, Vibrio cholerae O1/genetics/*metabolism | Genetics |
| Genetics | 32860313 | Comparison of chitin-induced natural transformation in pandemic Vibrio cholerae O1 El Tor strains. | Stutzmann S, Blokesch M | Environ Microbiol | 10.1111/1462-2920.15214 | 2020 | *Chitin, Cholera/epidemiology, Genome, Bacterial, Genomics, Humans, Pandemics, Phylogeny, *Vibrio cholerae O1/genetics/isolation & purification | Phylogeny |
| Metabolism | 33735319 | Phosphatidic acid-mediated binding and mammalian cell internalization of the Vibrio cholerae cytotoxin MakA. | Nadeem A, Alam A, Toh E, Myint SL, Ur Rehman Z, Liu T, Bally M, Arnqvist A, Wang H, Zhu J, Persson K, Uhlin BE, Wai SN | PLoS Pathog | 10.1371/journal.ppat.1009414 | 2021 | Animals, Bacterial Proteins/*metabolism, Cell Line, Cholera/metabolism, Cytotoxins/*metabolism, Humans, Mice, Phosphatidic Acids/*metabolism, Vibrio cholerae/*pathogenicity, Virulence Factors/*metabolism, Virus Internalization | Pathogenicity |
| 36090081 | Experimental evolution of Vibrio cholerae identifies hypervesiculation as a way to increase motility in the presence of polymyxin B. | Giacomucci S, Mathieu-Denoncourt A, Vincent AT, Jannadi H, Duperthuy M | Front Microbiol | 10.3389/fmicb.2022.932165 | 2022 |
Reference
| @id | authors | title | doi/url | catalogue |
|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
| 42477 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-106276 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 106276) | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
| 68368 | Automatically annotated from API 20E |