Strain identifier

BacDive ID: 13982

Type strain: No

Species: Azospirillum largimobile

Strain Designation: Sp A3a

Strain history: <- N.R. Krieg, Sp A3a <- J. Döbereiner

NCBI tax ID(s): 52761 (species)

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General

@ref: 897

BacDive-ID: 13982

DSM-Number: 1838

keywords: Bacteria, mesophilic

description: Azospirillum largimobile Sp A3a is a mesophilic bacterium that was isolated from grass roots.

NCBI tax id

  • NCBI tax id: 52761
  • Matching level: species

strain history

  • @ref: 897
  • history: <- N.R. Krieg, Sp A3a <- J. Döbereiner

doi: 10.13145/bacdive13982.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Rhodospirillales
  • family: Azospirillaceae
  • genus: Azospirillum
  • species: Azospirillum largimobile
  • full scientific name: Azospirillum largimobile corrig. (Skerman et al. 1983) Ben Dekhil et al. 1997

  • synonyms

    @refsynonym
    20215Conglomeromonas largomobilis
    20215Azospirillum largomobile

@ref: 897

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhodospirillales

family: Azospirillaceae

genus: Azospirillum

species: Azospirillum largimobile

full scientific name: Azospirillum largimobile (Skerman et al. 1983) Ben Dekhil et al. 1997

strain designation: Sp A3a

type strain: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
897HETEROTROPHIC MEDIUM H3P (DSMZ Medium 428)yeshttps://mediadive.dsmz.de/medium/428Name: HETEROTROPHIC MEDIUM H3P (DSMZ Medium 428) Composition: Agar 19.802 g/l Na2HPO4 x 2 H2O 2.87129 g/l KH2PO4 2.27723 g/l D-Glucose 1.9802 g/l Na-lactate 0.990099 g/l Na-pyruvate 0.990099 g/l D-Mannitol 0.990099 g/l DL-Malate 0.990098 g/l Na-acetate 0.990098 g/l Yeast extract 0.990098 g/l NH4Cl 0.990098 g/l Disodium succinate 0.990098 g/l MgSO4 x 7 H2O 0.495049 g/l Ferric ammonium citrate 0.049505 g/l CaCl2 x 2 H2O 0.00990098 g/l NaVO3 x H2O 0.00495049 g/l Thiamine-HCl x 2 H2O 0.00247525 g/l Nicotinic acid 0.00247525 g/l Pyridoxine hydrochloride 0.00247525 g/l Calcium pantothenate 0.00247525 g/l H3BO3 0.00148515 g/l CoCl2 x 6 H2O 0.000990099 g/l Riboflavin 0.00049505 g/l ZnSO4 x 7 H2O 0.00049505 g/l MnCl2 x 4 H2O 0.000148515 g/l Na2MoO4 x 2 H2O 0.000148515 g/l NiCl2 x 6 H2O 9.90099e-05 g/l CuCl2 x 2 H2O 4.95049e-05 g/l Vitamin B12 4.95049e-05 g/l Folic acid 9.90099e-06 g/l Biotin 4.9505e-06 g/l Distilled water
897MINERAL MEDIUM FOR CHEMOLITHOTROPHIC GROWTH (H-3) (DSMZ Medium 81)yeshttps://mediadive.dsmz.de/medium/81Name: MINERAL MEDIUM FOR CHEMOLITHOTROPHIC GROWTH (H-3) (DSMZ Medium 81) Composition: Agar 20.1005 g/l Na2HPO4 x 2 H2O 2.91457 g/l KH2PO4 2.31156 g/l NH4Cl 1.00503 g/l MgSO4 x 7 H2O 0.502512 g/l Ferric ammonium citrate 0.0502513 g/l CaCl2 x 2 H2O 0.0100503 g/l NaVO3 x H2O 0.00502512 g/l Calcium pantothenate 0.00251256 g/l Pyridoxine hydrochloride 0.00251256 g/l Nicotinic acid 0.00251256 g/l Thiamine-HCl x 2 H2O 0.00251256 g/l H3BO3 0.00150754 g/l CoCl2 x 6 H2O 0.00100503 g/l Riboflavin 0.000502513 g/l ZnSO4 x 7 H2O 0.000502513 g/l MnCl2 x 4 H2O 0.000150754 g/l Na2MoO4 x 2 H2O 0.000150754 g/l NiCl2 x 6 H2O 0.000100503 g/l CuCl2 x 2 H2O 5.02513e-05 g/l Vitamin B12 5.02513e-05 g/l Folic acid 1.00503e-05 g/l Biotin 5.02513e-06 g/l Distilled water
897AZOSPIRILLUM MEDIUM (DSMZ Medium 221)yeshttps://mediadive.dsmz.de/medium/221Name: AZOSPIRILLUM MEDIUM (DSMZ Medium 221) Composition: Agar 15.0 g/l Na malate 5.0 g/l Glucose 5.0 g/l (NH4)2SO4 1.0 g/l K2HPO4 0.25 g/l MgSO4 x 7 H2O 0.2 g/l NaCl 0.1 g/l Yeast extract 0.05 g/l CaCl2 x 2 H2O 0.02 g/l FeSO4 x 7 H2O 0.01 g/l MnSO4 x H2O 0.002 g/l Na2MoO4 x 2 H2O 0.001 g/l Biotin 0.0001 g/l Distilled water

culture temp

  • @ref: 897
  • growth: positive
  • type: growth
  • temperature: 30
  • range: mesophilic

Isolation, sampling and environmental information

isolation

  • @ref: 897
  • sample type: grass roots
  • geographic location: Dakar
  • country: Senegal
  • origin.country: SEN
  • continent: Africa

isolation source categories

Cat1Cat2Cat3
#Host#Plants#Herbaceous plants (Grass,Crops)
#Host Body-Site#Plant#Root (Rhizome)

Safety information

risk assessment

  • @ref: 897
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

External links

@ref: 897

culture collection no.: DSM 1838

straininfo link

  • @ref: 83153
  • straininfo: 47502

Reference

@idauthorscataloguedoi/urltitle
897Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 1838)https://www.dsmz.de/collection/catalogue/details/culture/DSM-1838
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
83153Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID47502.1StrainInfo: A central database for resolving microbial strain identifiers