Strain identifier
BacDive ID: 13981
Type strain: 
Species: Azospirillum halopraeferens
Strain Designation: AU4
Strain history: <- B. Reinhold, AU4
NCBI tax ID(s): 1121033 (strain), 34010 (species)
version 8.1 (current version)
General
@ref: 1452
BacDive-ID: 13981
DSM-Number: 3675
keywords: genome sequence, 16S sequence, Bacteria, mesophilic, motile
description: Azospirillum halopraeferens AU4 is a mesophilic, motile bacterium that was isolated from roots of Kallar grass Kunth).
NCBI tax id
| NCBI tax id | Matching level |
|---|---|
| 34010 | species |
| 1121033 | strain |
strain history
- @ref: 1452
- history: <- B. Reinhold, AU4
doi: 10.13145/bacdive13981.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Alphaproteobacteria
- order: Rhodospirillales
- family: Azospirillaceae
- genus: Azospirillum
- species: Azospirillum halopraeferens
- full scientific name: Azospirillum halopraeferens Reinhold et al. 1987
@ref: 1452
domain: Bacteria
phylum: Proteobacteria
class: Alphaproteobacteria
order: Rhodospirillales
family: Azospirillaceae
genus: Azospirillum
species: Azospirillum halopraeferens
full scientific name: Azospirillum halopraeferens Reinhold et al. 1987
strain designation: AU4
type strain: yes
Morphology
cell morphology
| @ref | motility | confidence | gram stain |
|---|---|---|---|
| 69480 | yes | 98.167 | |
| 69480 | 99.996 | negative |
Culture and growth conditions
culture medium
- @ref: 1452
- name: HETEROTROPHIC MEDIUM H3P (DSMZ Medium 428)
- growth: yes
- link: https://mediadive.dsmz.de/medium/428
- composition: Name: HETEROTROPHIC MEDIUM H3P (DSMZ Medium 428) Composition: Agar 19.802 g/l Na2HPO4 x 2 H2O 2.87129 g/l KH2PO4 2.27723 g/l D-Glucose 1.9802 g/l Na-lactate 0.990099 g/l Na-pyruvate 0.990099 g/l D-Mannitol 0.990099 g/l DL-Malate 0.990098 g/l Na-acetate 0.990098 g/l Yeast extract 0.990098 g/l NH4Cl 0.990098 g/l Disodium succinate 0.990098 g/l MgSO4 x 7 H2O 0.495049 g/l Ferric ammonium citrate 0.049505 g/l CaCl2 x 2 H2O 0.00990098 g/l NaVO3 x H2O 0.00495049 g/l Thiamine-HCl x 2 H2O 0.00247525 g/l Nicotinic acid 0.00247525 g/l Pyridoxine hydrochloride 0.00247525 g/l Calcium pantothenate 0.00247525 g/l H3BO3 0.00148515 g/l CoCl2 x 6 H2O 0.000990099 g/l Riboflavin 0.00049505 g/l ZnSO4 x 7 H2O 0.00049505 g/l MnCl2 x 4 H2O 0.000148515 g/l Na2MoO4 x 2 H2O 0.000148515 g/l NiCl2 x 6 H2O 9.90099e-05 g/l CuCl2 x 2 H2O 4.95049e-05 g/l Vitamin B12 4.95049e-05 g/l Folic acid 9.90099e-06 g/l Biotin 4.9505e-06 g/l Distilled water
culture temp
- @ref: 1452
- growth: positive
- type: growth
- temperature: 37
- range: mesophilic
Physiology and metabolism
spore formation
- @ref: 69480
- spore formation: no
- confidence: 99.201
Isolation, sampling and environmental information
isolation
- @ref: 1452
- sample type: roots of Kallar grass (Leptochloa fusca (Linn.) Kunth)
- host species: Leptochloa fusca
- geographic location: Punjab
- country: Pakistan
- origin.country: PAK
- continent: Asia
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Host | #Plants | #Herbaceous plants (Grass,Crops) |
| #Host Body-Site | #Plant | #Root (Rhizome) |
taxonmaps
- @ref: 69479
- File name: preview.99_36100.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_47;96_17280;97_21241;98_26619;99_36100&stattab=map
- Last taxonomy: Azospirillum halopraeferens subclade
- 16S sequence: GU256439
- Sequence Identity:
- Total samples: 14
- soil counts: 1
- aquatic counts: 8
- animal counts: 3
- plant counts: 2
Safety information
risk assessment
- @ref: 1452
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 20218 | A.halopraeferens (Au4) 16S rRNA gene | X79731 | 1323 | ena | 34010 |
| 20218 | Azospirillum halopraeferens strain DSM 3675 16S ribosomal RNA gene, partial sequence | GU256439 | 1393 | ena | 34010 |
| 20218 | A.halopraeferens (DSM 3675) 16S ribosomal RNA | Z29618 | 1417 | ena | 34010 |
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Azospirillum halopraeferens DSM 3675 | GCA_000429625 | scaffold | ncbi | 1121033 |
| 66792 | Azospirillum halopraeferens DSM 3675 | 1121033.3 | wgs | patric | 1121033 |
| 66792 | Azospirillum halopraeferens DSM 3675 | 2522572158 | draft | img | 1121033 |
GC content
- @ref: 1452
- GC-content: 68.1
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | no | 89 | no |
| motile | yes | 84.743 | no |
| flagellated | yes | 62.988 | no |
| gram-positive | no | 98.856 | no |
| anaerobic | no | 88.944 | no |
| aerobic | yes | 58.045 | no |
| halophile | no | 90.981 | no |
| spore-forming | no | 90.758 | no |
| thermophile | no | 93.335 | yes |
| glucose-util | yes | 86.763 | no |
| glucose-ferment | no | 87.264 | no |
External links
@ref: 1452
culture collection no.: DSM 3675, ATCC 43709, LMG 7108, CCUG 45018
straininfo link
- @ref: 83152
- straininfo: 1052
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 1452 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 3675) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-3675 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 83152 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID1052.1 | StrainInfo: A central database for resolving microbial strain identifiers |