Strain identifier
BacDive ID: 13972
Type strain: 
Species: Azospirillum brasilense
Strain Designation: Sp 7
Strain history: ATCC 29145 <-- J. Döbereiner Sp 7.
NCBI tax ID(s): 192 (species)
version 9.3 (current version)
General
@ref: 862
BacDive-ID: 13972
DSM-Number: 1690
keywords: genome sequence, 16S sequence, Bacteria, mesophilic, Gram-negative
description: Azospirillum brasilense Sp 7 is a mesophilic, Gram-negative bacterium that was isolated from Digitaria decumbens roots.
NCBI tax id
- NCBI tax id: 192
- Matching level: species
strain history
| @ref | history |
|---|---|
| 862 | <- ATCC <- J. Döbereiner, Sp 7 |
| 67770 | ATCC 29145 <-- J. Döbereiner Sp 7. |
doi: 10.13145/bacdive13972.20250331.9.3
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Alphaproteobacteria
- order: Rhodospirillales
- family: Azospirillaceae
- genus: Azospirillum
- species: Azospirillum brasilense
- full scientific name: Azospirillum brasilense corrig. Tarrand et al. 1979 (Approved Lists 1980)
synonyms
@ref synonym 20215 Roseomonas fauriae 20215 Azospirillum brasiliense
@ref: 862
domain: Bacteria
phylum: Proteobacteria
class: Alphaproteobacteria
order: Rhodospirillales
family: Azospirillaceae
genus: Azospirillum
species: Azospirillum brasilense
full scientific name: Azospirillum brasilense Tarrand et al. 1979
strain designation: Sp 7
type strain: yes
Morphology
cell morphology
| @ref | gram stain | confidence |
|---|---|---|
| 125438 | negative | 98.333 |
| 125439 | negative | 97.4 |
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 862 | R2A MEDIUM (DSMZ Medium 830) | yes | https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium830.pdf | |
| 862 | SSE/HD1:10 (DSMZ Medium 1426) | yes | https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1426.pdf | |
| 862 | HETEROTROPHIC MEDIUM H3P (DSMZ Medium 428) | yes | https://mediadive.dsmz.de/medium/428 | Name: HETEROTROPHIC MEDIUM H3P (DSMZ Medium 428) Composition: Agar 19.802 g/l Na2HPO4 x 2 H2O 2.87129 g/l KH2PO4 2.27723 g/l D-Glucose 1.9802 g/l Na-lactate 0.990099 g/l Na-pyruvate 0.990099 g/l D-Mannitol 0.990099 g/l DL-Malate 0.990098 g/l Na-acetate 0.990098 g/l Yeast extract 0.990098 g/l NH4Cl 0.990098 g/l Disodium succinate 0.990098 g/l MgSO4 x 7 H2O 0.495049 g/l Ferric ammonium citrate 0.049505 g/l CaCl2 x 2 H2O 0.00990098 g/l NaVO3 x H2O 0.00495049 g/l Thiamine-HCl x 2 H2O 0.00247525 g/l Nicotinic acid 0.00247525 g/l Pyridoxine hydrochloride 0.00247525 g/l Calcium pantothenate 0.00247525 g/l H3BO3 0.00148515 g/l CoCl2 x 6 H2O 0.000990099 g/l Riboflavin 0.00049505 g/l ZnSO4 x 7 H2O 0.00049505 g/l MnCl2 x 4 H2O 0.000148515 g/l Na2MoO4 x 2 H2O 0.000148515 g/l NiCl2 x 6 H2O 9.90099e-05 g/l CuCl2 x 2 H2O 4.95049e-05 g/l Vitamin B12 4.95049e-05 g/l Folic acid 9.90099e-06 g/l Biotin 4.9505e-06 g/l Distilled water |
culture temp
| @ref | growth | type | temperature |
|---|---|---|---|
| 862 | positive | growth | 28 |
| 862 | positive | growth | 30 |
| 67770 | positive | growth | 30 |
Physiology and metabolism
oxygen tolerance
- @ref: 125439
- oxygen tolerance: obligate aerobe
- confidence: 96.7
spore formation
- @ref: 125439
- spore formation: no
- confidence: 96.6
compound production
- @ref: 862
- compound: nitrogenase
Isolation, sampling and environmental information
isolation
| @ref | sample type | host species | country | origin.country | continent | geographic location |
|---|---|---|---|---|---|---|
| 862 | Digitaria decumbens roots | Digitaria decumbens | Brazil | BRA | Middle and South America | |
| 67770 | Digitaria decumbens roots | Digitaria decumbens | Brazil | BRA | Middle and South America | Rio de Janeiro |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Host | #Plants | #Herbaceous plants (Grass,Crops) |
| #Host Body-Site | #Plant | #Root (Rhizome) |
Safety information
risk assessment
- @ref: 862
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 20218 | Azospirillum brasilense strain ATCC 29145 16S ribosomal RNA gene, partial sequence | AY324110 | 1442 | nuccore | 192 |
| 20218 | Azospirillum brasilense strain DSM 1690 16S ribosomal RNA gene, partial sequence | GU256438 | 1400 | nuccore | 192 |
| 20218 | Azospirillum brasilense gene for 16S rRNA, partial sequence, strain: NBRC 102289 | AB681745 | 1265 | nuccore | 192 |
| 20218 | A.brasilense (NCIMB 11860) 16S ribosomal RNA | Z29617 | 1461 | nuccore | 192 |
| 20218 | A.brasilense (Sp7) 16S rRNA gene | X79739 | 1403 | nuccore | 192 |
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Azospirillum brasilense strain Sp 7 strain Sp7 | 192.73 | plasmid | patric | 192 |
| 66792 | Azospirillum brasilense strain Sp 7 strain Sp7 | 192.76 | plasmid | patric | 192 |
| 66792 | Azospirillum brasilense strain Sp 7 strain Sp7 | 192.77 | plasmid | patric | 192 |
| 66792 | Azospirillum brasilense strain Sp 7 strain Sp7 | 192.75 | plasmid | patric | 192 |
| 66792 | Azospirillum brasilense strain Sp 7 strain Sp7 | 192.74 | plasmid | patric | 192 |
| 66792 | Azospirillum brasilense strain Sp7 | 192.50 | complete | patric | 192 |
| 66792 | Azospirillum sp. Sp 7 | 1685931.3 | wgs | patric | 1685931 |
| 66792 | Azospirillum sp. TSA6c | 709813.3 | wgs | patric | 709813 |
| 66792 | Azospirillum sp. TSH100 | 652764.3 | wgs | patric | 652764 |
| 66792 | Azospirillum sp. TSH20 | 652754.3 | wgs | patric | 652754 |
| 66792 | Azospirillum sp. TSH58 | 664962.4 | wgs | patric | 664962 |
| 66792 | Azospirillum sp. TSH64 | 652740.3 | wgs | patric | 652740 |
| 66792 | Azospirillum sp. TSH7 | 652751.3 | wgs | patric | 652751 |
| 66792 | Azospirillum sp. TSO22-1 | 716789.3 | wgs | patric | 716789 |
| 66792 | Azospirillum sp. TSO35-2 | 716796.3 | wgs | patric | 716796 |
| 66792 | Azospirillum sp. TSO5 | 716760.3 | wgs | patric | 716760 |
| 66792 | Azospirillum brasilense DSM 1690 | 2596583658 | draft | img | 192 |
| 67770 | Azospirillum brasilense Sp 7 | GCA_002027385 | contig | ncbi | 192 |
| 66792 | Azospirillum brasilense Sp 7 | GCA_001315015 | complete | ncbi | 192 |
| 66792 | Azospirillum brasilense Sp 7 | GCA_007827425 | contig | ncbi | 192 |
| 66792 | Azospirillum brasilense Sp 7 | GCA_008274945 | complete | ncbi | 192 |
GC content
| @ref | GC-content | method |
|---|---|---|
| 862 | 70.0 | |
| 862 | 67.5 | |
| 67770 | 67.5 | thermal denaturation, midpoint method (Tm) |
| 67770 | 70 | thermal denaturation, midpoint method (Tm) |
Genome-based predictions
predictions
| @ref | model | trait | description | prediction | confidence | training_data |
|---|---|---|---|---|---|---|
| 125438 | gram-positive | gram-positive | Positive reaction to Gram-staining | no | 98.333 | no |
| 125438 | anaerobic | anaerobic | Ability to grow under anoxygenic conditions (including facultative anaerobes) | no | 84.14 | no |
| 125438 | spore-forming | spore-forming | Ability to form endo- or exospores | no | 82.468 | no |
| 125438 | aerobic | aerobic | Ability to grow under oxygenic conditions (including facultative aerobes) | yes | 72.228 | no |
| 125438 | thermophile | thermophilic | Ability to grow at temperatures above or equal to 45°C | no | 96.229 | yes |
| 125438 | motile2+ | flagellated | Ability to perform flagellated movement | yes | 80.894 | no |
| 125439 | BacteriaNet | spore_formation | Ability to form endo- or exospores | no | 96.6 | |
| 125439 | BacteriaNet | motility | Ability to perform movement | yes | 77.2 | |
| 125439 | BacteriaNet | gram_stain | Reaction to gram-staining | negative | 97.4 | |
| 125439 | BacteriaNet | oxygen_tolerance | Oxygenic conditions needed for growth | obligate aerobe | 96.7 |
External links
@ref: 862
culture collection no.: DSM 1690, ATCC 29145, JCM 1224, BCRC 12270, CCM 3862, CECT 590, IAM 12400, ICMP 8671, LMG 1263, LMG 13127, NBRC 102289, NCIMB 11860, NRRL B-14647, VKM B-1547
straininfo link
- @ref: 83143
- straininfo: 7462
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 356945 | A taxonomic study of the Spirillum lipoferum group, with descriptions of a new genus, Azospirillum gen. nov. and two species, Azospirillum lipoferum (Beijerinck) comb. nov. and Azospirillum brasilense sp. nov. | Tarrand JJ, Krieg NR, Dobereiner J | Can J Microbiol | 10.1139/m78-160 | 1978 | Culture Media, Culture Techniques, DNA, Bacterial/*analysis, Microscopy, Electron, Microscopy, Phase-Contrast, Nucleic Acid Denaturation, Spirillum/*classification/cytology/physiology, Terminology as Topic | Cultivation |
| Enzymology | 2675764 | Cloning and expression in Escherichia coli of the Azospirillum brasilense Sp7 gene encoding ampicillin resistance. | Verreth C, Cammue B, Swinnen P, Crombez D, Michielsen A, Michiels K, Van Gool A, Vanderleyden J | Appl Environ Microbiol | 10.1128/aem.55.8.2056-2060.1989 | 1989 | Ampicillin Resistance/*genetics, Blotting, Southern, Centrifugation, Density Gradient, Cloning, Molecular, DNA, Bacterial/genetics, Escherichia coli/drug effects/enzymology/*genetics, *Gene Expression Regulation, Genes, Bacterial, Gram-Negative Bacteria/drug effects/enzymology/*genetics, Nucleic Acid Hybridization, Restriction Mapping, beta-Lactamases/biosynthesis/*genetics | Pathogenicity |
| Enzymology | 2893582 | Cloning and characterization of the glnA gene of Azospirillum brasilense Sp7. | Bozouklian H, Fogher C, Elmerich C | Ann Inst Pasteur Microbiol (1985) | 10.1016/s0769-2609(86)80089-8 | 1986 | *Cloning, Molecular, *Genes, *Genes, Bacterial, Glutamate-Ammonia Ligase/genetics, Nitrogen Fixation/genetics, Plasmids, Spirillum/*genetics | |
| Cultivation | 3104311 | Cyst production and brown pigment formation in aging cultures of Azospirillum brasilense ATCC 29145. | Sadasivan L, Neyra CA | J Bacteriol | 10.1128/jb.169.4.1670-1677.1987 | 1987 | Bacteria/*cytology/metabolism/ultrastructure, Centrifugation, Density Gradient, Culture Media, Cytoplasmic Granules/ultrastructure, Hydroxybutyrates/analysis, Microscopy, Electron, Pigments, Biological/analysis/*biosynthesis, *Polyesters, Polymers/analysis | Biotechnology |
| Phylogeny | 3182731 | Isolation and characterization of Azospirillum brasilense loci that correct Rhizobium meliloti exoB and exoC mutations. | Michiels KW, Vanderleyden J, Van Gool AP, Signer ER | J Bacteriol | 10.1128/jb.170.11.5401-5404.1988 | 1988 | *Genes, *Genes, Bacterial, Genetic Complementation Test, *Mutation, Nitrogenase/genetics, Polysaccharides, Bacterial/*genetics, Rhizobium/*genetics, Spirillum/*genetics | |
| Phylogeny | 3894333 | Flocculation in Azospirillum brasilense and Azospirillum lipoferum: exopolysaccharides and cyst formation. | Sadasivan L, Neyra CA | J Bacteriol | 10.1128/jb.163.2.716-723.1985 | 1985 | Aerobiosis, Cell Survival, Flocculation, Gram-Negative Aerobic Bacteria/cytology/growth & development/*physiology, Microscopy, Electron, Microscopy, Phase-Contrast, Polysaccharides, Bacterial/*analysis, Species Specificity, Spores, Bacterial/physiology | |
| Metabolism | 6425263 | Intermediary carbon metabolism of Azospirillum brasilense. | Loh WH, Randles CI, Sharp WR, Miller RH | J Bacteriol | 10.1128/jb.158.1.264-268.1984 | 1984 | Acetates/*metabolism, Acetyl Coenzyme A/metabolism, Bacteria/enzymology/*metabolism, Carbon Dioxide/metabolism, Cell Wall/metabolism, Citric Acid Cycle, Culture Media, Gluconeogenesis, Glucose/*metabolism, Kinetics, Lipids/biosynthesis, Oxygen Consumption, Pyruvate Dehydrogenase Complex/metabolism, Pyruvates/*metabolism, Pyruvic Acid, Succinates/*metabolism, Succinic Acid | Enzymology |
| Metabolism | 6588050 | Catabolism of carbohydrates and organic acids and expression of nitrogenase by azospirilla. | Martinez-Drets G, Del Gallo M, Burpee C, Burris RH | J Bacteriol | 10.1128/jb.159.1.80-85.1984 | 1984 | *Carbohydrate Metabolism, Carbon Radioisotopes, Carboxylic Acids/*metabolism, Citric Acid Cycle, Fructose/metabolism, Glucose/metabolism, Gram-Negative Aerobic Bacteria/genetics/*metabolism, Hexosephosphates/metabolism, Ketoglutaric Acids/metabolism, Kinetics, Nitrogenase/*genetics, Species Specificity, Sucrose/metabolism | Enzymology |
| Metabolism | 6789740 | Physiological properties and plasmid content of several strains of Azospirillum brasilense and A. lipoferum. | Franche C, Elmerich C | Ann Microbiol (Paris) | 1981 | Bacteria/metabolism, *Bacterial Physiological Phenomena, Bacteriophages/isolation & purification, Lysogeny/drug effects, Microbial Sensitivity Tests, Mitomycin, Mitomycins/pharmacology, Molecular Weight, Nitrogen Fixation, *Plasmids | Enzymology | |
| Enzymology | 6812526 | Enhancement of specific nitrogenase activity in Azospirillum brasilense and Klebsiella pneumoniae, inhibition in Rhizobium japonicum under air by phenol. | Werner D, Krotzky A, Berggold R, Thierfelder H, Preiss M | Arch Microbiol | 10.1007/BF00690817 | 1982 | Bacteria/drug effects/*enzymology, Klebsiella pneumoniae/drug effects/*enzymology, Nitrogenase/*analysis, Phenol, Phenols/metabolism/*pharmacology, Rhizobium/drug effects/*enzymology | Pathogenicity |
| Pathogenicity | 11949252 | [Effect of Azospirillum brasilense lectin on the kinetics of lymph nodes cell populations and cytokine status in experimental animals]. | Nikitina VE, Bugaeva IO, Ponomareva EG, Tikhomirova EI, Bogomolova NV | Zh Mikrobiol Epidemiol Immunobiol | 2002 | Animals, Azospirillum brasilense, Cell Cycle/drug effects, Cytokines/*drug effects/*metabolism, Lectins/isolation & purification/*pharmacology, Lymph Nodes/*cytology/*drug effects, Lymphocyte Count, Male, Mice | Enzymology | |
| Phylogeny | 15213753 | Molecular and physiological comparison of Azospirillum spp. isolated from Rhizoctonia solani mycelia, wheat rhizosphere, and human skin wounds. | Cohen MF, Han XY, Mazzola M | Can J Microbiol | 10.1139/w04-007 | 2004 | Azospirillum/*classification/genetics/isolation & purification/physiology, Culture Media, Humans, Plant Roots/*microbiology, Rhizoctonia, Sequence Analysis, DNA, Skin/injuries, Wounds and Injuries/*microbiology | Cultivation |
| 16345490 | Association of azospirillum with grass roots. | Umali-Garcia M, Hubbell DH, Gaskins MH, Dazzo FB | Appl Environ Microbiol | 10.1128/aem.39.1.219-226.1980 | 1980 | |||
| 16346311 | Enhanced Mineral Uptake by Zea mays and Sorghum bicolor Roots Inoculated with Azospirillum brasilense. | Lin W, Okon Y, Hardy RW | Appl Environ Microbiol | 10.1128/aem.45.6.1775-1779.1983 | 1983 | |||
| 16346482 | Application of the Indirect Immunoperoxidase Stain Technique to the Flagella of Azospirillum brasilense. | Hall PG, Krieg NR | Appl Environ Microbiol | 10.1128/aem.47.2.433-435.1984 | 1984 | |||
| Metabolism | 16347043 | Comparison of Two Cellulomonas Strains and Their Interaction with Azospirillum brasilense in Degradation of Wheat Straw and Associated Nitrogen Fixation. | Halsall DM, Gibson AH | Appl Environ Microbiol | 10.1128/aem.51.4.855-861.1986 | 1986 | ||
| 16349041 | Chemotaxis of azospirillum species to aromatic compounds. | Lopez-de-Victoria G, Lovell CR | Appl Environ Microbiol | 10.1128/aem.59.9.2951-2955.1993 | 1993 | |||
| Phylogeny | 21742820 | Azospirillum formosense sp. nov., a diazotroph from agricultural soil. | Lin SY, Shen FT, Young LS, Zhu ZL, Chen WM, Young CC | Int J Syst Evol Microbiol | 10.1099/ijs.0.030585-0 | 2011 | Azospirillum brasilense/*classification/genetics/*isolation & purification/metabolism, Bacterial Typing Techniques, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Molecular Sequence Data, Nitrogen Fixation, Nucleic Acid Hybridization, Phospholipids/analysis, Phylogeny, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Taiwan | Genetics |
| Pathogenicity | 22991739 | Osmoregulation in Azospirillum brasilense: glycine betaine transport enhances growth and nitrogen fixation under salt stress. | Riou N, Le Rudulier D | J Gen Microbiol | 10.1099/00221287-136-8-1455 | 1990 | Azospirillum brasilense/*drug effects/*metabolism, *Betaine/metabolism/pharmacology, Biological Transport, Kinetics, Nitrogen Fixation/*drug effects, Periplasm/metabolism, Protein Binding, Salts/pharmacology, Stress, Physiological/*drug effects, Water-Electrolyte Balance/*drug effects | Metabolism |
| Phylogeny | 23645021 | Azospirillum fermentarium sp. nov., a nitrogen-fixing species isolated from a fermenter. | Lin SY, Liu YC, Hameed A, Hsu YH, Lai WA, Shen FT, Young CC | Int J Syst Evol Microbiol | 10.1099/ijs.0.050872-0 | 2013 | Azospirillum/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, Bioreactors/*microbiology, DNA, Bacterial/genetics, Fatty Acids/analysis, *Fermentation, Molecular Sequence Data, Nitrogen Fixation, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Taiwan, Ubiquinone/analysis | Genetics |
| Metabolism | 28559297 | Characterization of a Novel cis-3-Hydroxy-l-Proline Dehydratase and a trans-3-Hydroxy-l-Proline Dehydratase from Bacteria. | Watanabe S, Fukumori F, Miyazaki M, Tagami S, Watanabe Y | J Bacteriol | 10.1128/JB.00255-17 | 2017 | Azospirillum brasilense/*enzymology/genetics/growth & development/metabolism, Carbon/metabolism, Gene Knockout Techniques, Hydro-Lyases/*isolation & purification/*metabolism, Hydroxyproline/genetics/*metabolism, Multigene Family, Protein Subunits/genetics/metabolism, Transcription, Genetic | Enzymology |
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 862 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 1690) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-1690 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
| 83143 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID7462.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
| 125438 | Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann | 10.1101/2024.08.12.607695 | Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets | |||
| 125439 | Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy | https://github.com/GenomeNet/deepG | deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 |