Strain identifier

BacDive ID: 139519

Type strain: No

Species: Vibrio cholerae

Strain Designation: N16961

Strain history: CIP <- 2000, J.B. Kaper, Maryland Univ., Baltimore, Maryland, USA: strain N16961 <- J. Craig <- Cholera Res. Lab., Bangladesh

NCBI tax ID(s): 666 (species)

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General

@ref: 41792

BacDive-ID: 139519

keywords: Bacteria, facultative anaerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Vibrio cholerae N16961 is a facultative anaerobe, mesophilic, Gram-negative bacterium of the family Vibrionaceae.

NCBI tax id

  • NCBI tax id: 666
  • Matching level: species

strain history

  • @ref: 41792
  • history: CIP <- 2000, J.B. Kaper, Maryland Univ., Baltimore, Maryland, USA: strain N16961 <- J. Craig <- Cholera Res. Lab., Bangladesh

doi: 10.13145/bacdive139519.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Vibrionales
  • family: Vibrionaceae
  • genus: Vibrio
  • species: Vibrio cholerae
  • full scientific name: Vibrio cholerae Pacini 1854 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Vibrio albensis

@ref: 41792

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Vibrionales

family: Vibrionaceae

genus: Vibrio

species: Vibrio cholerae

strain designation: N16961

type strain: no

Morphology

cell morphology

  • @ref: 41792
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
41792MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
41792CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
41792positivegrowth30mesophilic
41792positivegrowth15-41
41792nogrowth5psychrophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 41792
  • oxygen tolerance: facultative anaerobe

halophily

@refsaltgrowthtested relationconcentration
41792NaClpositivegrowth0-6 %
41792NaClnogrowth8 %
41792NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4179216947citrate+carbon source
417924853esculin-hydrolysis
4179217234glucose+fermentation
4179217716lactose-fermentation
4179217632nitrate+reduction
4179216301nitrite-reduction
41792132112sodium thiosulfate-builds gas from
4179217234glucose+degradation
4179217632nitrate+respiration

antibiotic resistance

  • @ref: 41792
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

  • @ref: 41792
  • Chebi-ID: 35581
  • metabolite: indole
  • production: yes

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
4179215688acetoin+
4179217234glucose+

enzymes

@refvalueactivityec
41792oxidase+
41792beta-galactosidase+3.2.1.23
41792alcohol dehydrogenase-1.1.1.1
41792gelatinase+
41792amylase+
41792DNase+
41792caseinase+3.4.21.50
41792catalase+1.11.1.6
41792tween esterase+
41792gamma-glutamyltransferase+2.3.2.2
41792lecithinase+
41792lipase+
41792lysine decarboxylase+4.1.1.18
41792ornithine decarboxylase+4.1.1.17
41792phenylalanine ammonia-lyase-4.3.1.24
41792protease+
41792tryptophan deaminase+
41792urease-3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)-
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase-3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase-3.1.3.2
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
41792--------------------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
41792+++++--+-++------+-+-----------+-+--------------+++--+-----++--------------+---++-+---+--++++-+--++

Isolation, sampling and environmental information

isolation

@refcountryorigin.countrycontinentsample typeisolation date
41792BangladeshBGDAsia
41792BangladeshBGDAsiaHuman, Stool1975

Safety information

risk assessment

  • @ref: 41792
  • biosafety level: 2
  • biosafety level comment: Risk group (French classification)

External links

@ref: 41792

culture collection no.: CIP 106855, ATCC 39315

straininfo link

  • @ref: 96006
  • straininfo: 41492

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Pathogenicity25915771gbpA as a Novel qPCR Target for the Species-Specific Detection of Vibrio cholerae O1, O139, Non-O1/Non-O139 in Environmental, Stool, and Historical Continuous Plankton Recorder Samples.Vezzulli L, Stauder M, Grande C, Pezzati E, Verheye HM, Owens NJ, Pruzzo CPLoS One10.1371/journal.pone.01239832015Feces/*microbiology, *Genes, Bacterial, Humans, Microbiota/*genetics, Molecular Typing/*methods, Plankton/*microbiology, Polymerase Chain Reaction/methods, Seawater/*microbiology, Sensitivity and Specificity, Vibrio cholerae/classification/*genetics/isolation & purificationPhylogeny
32994896Aptamer-nanobody based ELASA for detection of Vibrio cholerae O1.Mojarad AE, Gargaria SLMIran J Microbiol10.18502/ijm.v12i4.39282020

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
41792Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106855Collection of Institut Pasteur (CIP 106855)
68382Automatically annotated from API zym
96006Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID41492.1