Strain identifier

BacDive ID: 139459

Type strain: Yes

Species: Legionella fairfieldensis

Strain Designation: 1725-Aus-E

Strain history: CIP <- 1997, CDC <- W.L. Thacker: strain 1725-Aus-E <- L. Hawes

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 41679

BacDive-ID: 139459

keywords: genome sequence, Bacteria, microaerophile, mesophilic, Gram-negative

description: Legionella fairfieldensis 1725-Aus-E is a microaerophile, mesophilic, Gram-negative bacterium of the family Legionellaceae.

NCBI tax id

NCBI tax idMatching level
1408444strain
45064species

strain history

  • @ref: 41679
  • history: CIP <- 1997, CDC <- W.L. Thacker: strain 1725-Aus-E <- L. Hawes

doi: 10.13145/bacdive139459.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Legionellales
  • family: Legionellaceae
  • genus: Legionella
  • species: Legionella fairfieldensis
  • full scientific name: Legionella fairfieldensis Thacker et al. 1991

@ref: 41679

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Legionellales

family: Legionellaceae

genus: Legionella

species: Legionella fairfieldensis

strain designation: 1725-Aus-E

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480negative99.999
41679negativerod-shapedyes

colony morphology

  • @ref: 63321
  • incubation period: 3-5 days

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
41679MEDIUM 23 - for Afipia and LegionellayesDistilled water make up to (1000.000 ml);Legionella agar (37.000 g);Legionella - enrichment mixture (10.000 ml)
41679CIP Medium 23yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=23

culture temp

@refgrowthtypetemperaturerange
41679positivegrowth37mesophilic
63321positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 63321
  • oxygen tolerance: microaerophile

spore formation

@refspore formationconfidence
69481no100
69480no99.995

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4167917632nitrate-reduction
4167916301nitrite-reduction

metabolite production

  • @ref: 41679
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
41679oxidase-
41679catalase+1.11.1.6
41679urease-3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase-3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
41679--++------++--------

Isolation, sampling and environmental information

isolation

@refgeographic locationcountryorigin.countrycontinentsample typesampling date
41679Fairfield, VictoriaAustraliaAUSAustralia and Oceania
63321Victoria,FairfieldAustraliaAUSAustralia and OceaniaWater,cooling tower1991
41679Fairfield, VictoriaAustraliaAUSAustralia and OceaniaEnvironment, Water, cooling tower

isolation source categories

Cat1Cat2Cat3
#Engineered#Built environment#Water reservoir (Aquarium/pool)
#Environmental#Aquatic

Safety information

risk assessment

  • @ref: 41679
  • biosafety level: 2
  • biosafety level comment: Risk group (French classification)

Sequence information

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Legionella fairfieldensis ATCC 49588GCA_000621525scaffoldncbi1408444
66792Legionella fairfieldensis ATCC 495881408444.3wgspatric1408444
66792Legionella fairfieldensis ATCC 495882558860982draftimg1408444

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileyes81.194no
flagellatedno82.607no
gram-positiveno98.771no
anaerobicno98.681yes
halophileno84.941no
spore-formingno93.417no
thermophileno93.444yes
glucose-utilyes60.176no
aerobicno91.68no
glucose-fermentno88.785no

External links

@ref: 41679

culture collection no.: CIP 105266, ATCC 49588, NCTC 12488, CCUG 31235, CCUG 66341, NCTC 14288

straininfo link

  • @ref: 95959
  • straininfo: 42567

literature

  • topic: Phylogeny
  • Pubmed-ID: 2037664
  • title: Legionella fairfieldensis sp. nov. isolated from cooling tower waters in Australia.
  • authors: Thacker WL, Benson RF, Hawes L, Gidding H, Dwyer B, Mayberry WR, Brenner DJ
  • journal: J Clin Microbiol
  • DOI: 10.1128/jcm.29.3.475-478.1991
  • year: 1991
  • mesh: Australia, DNA, Bacterial/genetics, Fatty Acids/analysis, Legionella/classification/*isolation & purification/physiology, Serotyping, Species Specificity, *Water Microbiology
  • topic2: Enzymology

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
41679Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105266Collection of Institut Pasteur (CIP 105266)
63321Curators of the CCUGhttps://www.ccug.se/strain?id=66341Culture Collection University of Gothenburg (CCUG) (CCUG 66341)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
95959Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID42567.1