Strain identifier

BacDive ID: 139435

Type strain: No

Species: Klebsiella pneumoniae subsp. ozaenae

Strain Designation: O45, E5051

Strain history: CIP <- 1952, I. Oerskov, Copenhagen, Denmark: strain E5051 <- E.P. Snijders, Amsterdam, The Netherlands: strain O45

NCBI tax ID(s): 574 (subspecies)

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General

@ref: 41629

BacDive-ID: 139435

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-negative

description: Klebsiella pneumoniae subsp. ozaenae O45 is a facultative anaerobe, mesophilic, Gram-negative bacterium of the family Enterobacteriaceae.

NCBI tax id

  • NCBI tax id: 574
  • Matching level: subspecies

strain history

  • @ref: 41629
  • history: CIP <- 1952, I. Oerskov, Copenhagen, Denmark: strain E5051 <- E.P. Snijders, Amsterdam, The Netherlands: strain O45

doi: 10.13145/bacdive139435.20250331.9.3

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Enterobacteriaceae
  • genus: Klebsiella
  • species: Klebsiella pneumoniae subsp. ozaenae
  • full scientific name: Klebsiella pneumoniae subsp. ozaenae (Abel 1893) Ørskov 1984

  • synonyms

    @refsynonym
    20215Bacillus ozaenae
    20215Klebsiella ozaenae

@ref: 41629

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacterales

family: Enterobacteriaceae

genus: Klebsiella

species: Klebsiella pneumoniae subsp. ozaenae

strain designation: O45, E5051

type strain: no

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
125438negative100
125439negative97.5
41629negativerod-shapedno

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
41629MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
41629CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3
41629CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

  • @ref: 41629
  • growth: positive
  • type: growth
  • temperature: 30

Physiology and metabolism

oxygen tolerance

  • @ref: 41629
  • oxygen tolerance: facultative anaerobe

spore formation

@refspore formationconfidence
125438no90.329
125439no95

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6837430911sorbitol+builds acid from
6837417057cellobiose+builds acid from
6837417268myo-inositol+builds acid from
6837462345L-rhamnose+builds acid from
6837427082trehalose+builds acid from
6837418333D-arabitol+builds acid from
6837417634D-glucose+builds acid from
6837427897tryptophan+energy source
6837418394palatinose-builds acid from
6837415792malonate-assimilation
68374Potassium 5-ketogluconate-builds acid from
6837418403L-arabitol-builds acid from
6837416199urea+hydrolysis
6837418257ornithine-degradation
4162917632nitrate+reduction
4162916301nitrite-reduction
6837430849L-arabinose-builds acid from
6837417992sucrose+builds acid from
6837415963ribitol+builds acid from
6837417306maltose+builds acid from
6837416899D-mannitol+builds acid from
6837418024D-galacturonic acid+builds acid from
6837425094lysine-degradation
6837429016arginine+hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6837435581indoleyes
4162935581indoleno

metabolite tests

  • @ref: 68374
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: +

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
41629oxidase-
41629catalase+1.11.1.6
41629urease-3.5.1.5
68374alpha-maltosidase+
68374alpha-galactosidase+3.2.1.22
68374N-acetyl-beta-glucosaminidase-3.2.1.52
68374L-aspartate arylamidase+3.4.11.21
68374alpha-glucosidase-3.2.1.20
68374beta-galactosidase+3.2.1.23
68374beta-glucuronidase-3.2.1.31
68374beta-glucosidase+3.2.1.21
68374lipase-
68374urease+3.5.1.5
68374lysine decarboxylase-4.1.1.18
68374arginine dihydrolase+3.5.3.6
68374ornithine decarboxylase-4.1.1.17

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
41629-++--+----++-+-++---

API ID32E

@refODCADH ArgLDC LysURELARLGAT5KGLIPRPbeta GLUMANMALADOPLEbeta GURMNTINDbeta NAGbeta GALGLUSACLARADARLalpha GLUalpha GALTRERHAINOCELSORalphaMALAspA
44196-+-+-+---++++---+-+++-+-++++++++

Isolation, sampling and environmental information

isolation

@refgeographic locationcountryorigin.countrycontinentisolation date
41629AmsterdamNetherlandsNLDEurope
41629AmsterdamNetherlandsNLDEurope1937

Safety information

risk assessment

  • @ref: 41629
  • biosafety level: 2
  • biosafety level comment: Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
124043Klebsiella pneumoniae subsp. ozaenae culture-collection ATCC:11297 clone L21 16S-23S ribosomal RNA intergenic spacer, partial sequence; and tRNA-Glu gene, complete sequence.EU623180343nuccore574
124043Klebsiella pneumoniae subsp. ozaenae culture-collection ATCC:11297 clone L31 16S-23S ribosomal RNA intergenic spacer, partial sequence; and tRNA-Glu gene, complete sequence.EU623181343nuccore574
124043Klebsiella pneumoniae subsp. ozaenae culture-collection ATCC:11297 clone L32 16S-23S ribosomal RNA intergenic spacer, partial sequence; and tRNA-Glu gene, complete sequence.EU623182343nuccore574
124043Klebsiella pneumoniae subsp. ozaenae culture-collection ATCC:11297 clone L24 16S-23S ribosomal RNA intergenic spacer, partial sequence; and tRNA-Ile and tRNA-Ala genes, complete sequence.EU623183427nuccore574
124043Klebsiella pneumoniae subsp. ozaenae culture-collection ATCC:11297 clone L23 16S-23S ribosomal RNA intergenic spacer, partial sequence; and tRNA-Ile and tRNA-Ala genes, complete sequence.EU623184427nuccore574
124043Klebsiella pneumoniae subsp. ozaenae culture-collection ATCC:11297 clone L37 16S-23S ribosomal RNA intergenic spacer, partial sequence; and tRNA-Ile and tRNA-Ala genes, complete sequence.EU623185427nuccore574
124043Klebsiella pneumoniae subsp. ozaenae culture-collection ATCC:11297 clone S4 16S-23S ribosomal RNA intergenic spacer, partial sequence.EU623186198nuccore574
124043Klebsiella pneumoniae subsp. ozaenae culture-collection ATCC:11297 clone S3 16S-23S ribosomal RNA intergenic spacer, partial sequence.EU623187198nuccore574
124043Klebsiella pneumoniae subsp. ozaenae culture-collection ATCC:11297 clone S7 16S-23S ribosomal RNA intergenic spacer, partial sequence.EU623188198nuccore574
124043Klebsiella pneumoniae subsp. ozaenae culture-collection ATCC:11297 clone S6 16S-23S ribosomal RNA intergenic spacer, partial sequence.EU623189198nuccore574
124043Klebsiella pneumoniae subsp. ozaenae culture-collection ATCC:11297 clone L28 16S-23S ribosomal RNA intergenic spacer, partial sequence.EU623190198nuccore574

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Klebsiella pneumoniae NCTC5051GCA_900451855contigncbi573
66792Klebsiella pneumoniae strain NCTC5051573.20147wgspatric573

Genome-based predictions

predictions

@refmodeltraitdescriptionpredictionconfidencetraining_data
125438gram-positivegram-positivePositive reaction to Gram-stainingno100no
125438anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no95.604yes
125438spore-formingspore-formingAbility to form endo- or exosporesno90.329no
125438thermophilethermophilicAbility to grow at temperatures above or equal to 45°Cno99.5yes
125438aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)no58.085no
125438motile2+flagellatedAbility to perform flagellated movementno77.005no
125439BacteriaNetspore_formationAbility to form endo- or exosporesno95
125439BacteriaNetmotilityAbility to perform movementyes75.1
125439BacteriaNetgram_stainReaction to gram-stainingnegative97.5
125439BacteriaNetoxygen_toleranceOxygenic conditions needed for growthaerobe79.6

External links

@ref: 41629

culture collection no.: CIP 52.212, ATCC 11297, NCTC 5051, CCUG 416

straininfo link

  • @ref: 95937
  • straininfo: 3091

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
41629Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2052.212Collection of Institut Pasteur (CIP 52.212)
44196Curators of the CCUGhttps://www.ccug.se/strain?id=416Culture Collection University of Gothenburg (CCUG) (CCUG 416)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar SchomburgBRENDA, the ELIXIR core data resource in 2021: new developments and updates10.1093/nar/gkaa1025Nucleic Acids Res. 49: D498-D508 202033211880
68374Automatically annotated from API ID32E
68382Automatically annotated from API zym
95937Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID3091.1
124043Isabel Schober, Julia KoblitzData extracted from sequence databases, automatically matched based on designation and taxonomy
125438Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg OvermannPredicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets10.1101/2024.08.12.607695
125439Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardydeepG: Deep Learning for Genome Sequence Data. R package version 0.3.1https://github.com/GenomeNet/deepG