Strain identifier

BacDive ID: 139291

Type strain: No

Species: Clostridium perfringens

Strain history: CIP <- 1999, J.P. Carlier, Inst. Pasteur, Paris, France: strain AIP 10201 <- NCTC <- B.C. Hobbs, PHLS, London, UK

NCBI tax ID(s): 1502 (species)

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General

@ref: 41430

BacDive-ID: 139291

keywords: genome sequence, Bacteria, anaerobe, spore-forming, mesophilic, Gram-positive

description: Clostridium perfringens CIP 106157 is an anaerobe, spore-forming, mesophilic bacterium of the family Clostridiaceae.

NCBI tax id

  • NCBI tax id: 1502
  • Matching level: species

strain history

  • @ref: 41430
  • history: CIP <- 1999, J.P. Carlier, Inst. Pasteur, Paris, France: strain AIP 10201 <- NCTC <- B.C. Hobbs, PHLS, London, UK

doi: 10.13145/bacdive139291.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Clostridiaceae
  • genus: Clostridium
  • species: Clostridium perfringens
  • full scientific name: Clostridium perfringens (Veillon and Zuber 1898) Hauduroy et al. 1937 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Bacillus perfringens

@ref: 41430

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Eubacteriales

family: Clostridiaceae

genus: Clostridium

species: Clostridium perfringens

type strain: no

Morphology

cell morphology

@refgram stainconfidencemotility
69480positive100
41430positiveno

colony morphology

  • @ref: 41430

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
41430MEDIUM 20 - for Anaerobic bacteriayesAgar (15.000 g);Glucose (5.000 g);Yeast extract (20.000 g);Tryptone (30.000 g);Cysteine hydrochloride (0.500 g);distilled water (1000.000 ml);Hemin solution -M00149 (25.000 ml)
41430CIP Medium 20yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=20

culture temp

  • @ref: 41430
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
69480anaerobe99.508
41430anaerobe

spore formation

@refspore formationconfidence
69481yes100
69480yes99.999
41430yes

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4143017108D-arabinose-degradation
4143015824D-fructose+/-degradation
4143017634D-glucose+/-degradation
4143065327D-xylose-degradation
4143017057cellobiose-degradation
4143017716lactose+/-degradation
4143017306maltose+/-degradation
4143017814salicin-degradation
4143017992sucrose+/-degradation
414304853esculin-hydrolysis
4143017632nitrate-reduction
4143016301nitrite+reduction
4143017632nitrate+respiration

antibiotic resistance

  • @ref: 41430
  • ChEBI: 6909
  • metabolite: metronidazole
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 41430
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
41430oxidase-
41430beta-galactosidase+3.2.1.23
41430gelatinase+/-
41430amylase-
41430DNase+
41430caseinase+3.4.21.50
41430catalase-1.11.1.6
41430tween esterase-
41430lecithinase+
41430lipase-
41430protease+
41430urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase-3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
41430-+++----+-++++-+-+--

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
41430---------------------------------------------------------------------------------------------------

Isolation, sampling and environmental information

isolation

@refcountryorigin.countrycontinentsample typeisolation date
41430United KingdomGBREurope
41430United KingdomGBREuropeFood, Meat rissole1953

Safety information

risk assessment

  • @ref: 41430
  • biosafety level: 2
  • biosafety level comment: Risk group (French classification)

Sequence information

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Clostridium perfringens NCTC8798GCA_900447105contigncbi1502
66792Clostridium perfringens strain NCTC87981502.485wgspatric1502

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
motileno84.641no
gram-positiveyes95.69no
anaerobicyes97.72no
aerobicno94.84no
halophileno66.573no
spore-formingyes90.694no
thermophileno97.455yes
glucose-utilyes90.474no
flagellatedno92.192no
glucose-fermentyes78.418no

External links

@ref: 41430

culture collection no.: CIP 106157, NCTC 8798, AIP 10201

straininfo link

  • @ref: 95829
  • straininfo: 67932

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Pathogenicity173240Repair of heat-injured Clostridium perfringens spores during outgrowth.Barach JT, Flowers RS, Adams DMAppl Microbiol10.1128/am.30.5.873-875.19751975Alkalies/pharmacology, Clostridium perfringens/drug effects/*growth & development, Culture Media, *Hot Temperature, Muramidase/pharmacology, Neomycin/pharmacology, Polymyxins/pharmacology, Spores, Bacterial/drug effects/growth & developmentCultivation
Metabolism180885Influence of starch source on sporulation and enterotoxin production by Clostridium perfringens type A.Labbe R, Somers E, Duncan CAppl Environ Microbiol10.1128/aem.31.3.455-457.19761976Clostridium perfringens/*growth & development/metabolism, Enterotoxins/*biosynthesis, Hot Temperature, Oryza, Spores, Bacterial/growth & development/metabolism, Starch/*metabolism, Triticum, Vegetables, Zea maysStress
Stress1369407Stimulation of the onset of sporulation of Clostridium perfringens type A by netropsin and distamycin.Ryu S, Labbe RGCurr Microbiol10.1007/BF015707171992Clostridium perfringens/*drug effects/physiology/ultrastructure, Distamycins/*pharmacology, Hot Temperature, Kinetics, Microscopy, Electron, Netropsin/*pharmacology, Spores, BacterialEnzymology
Enzymology4343403Biological characteristics of Clostridium perfringens type A enterotoxin.Stark RL, Duncan CLInfect Immun10.1128/iai.4.2.89-96.19711971Animals, Antitoxins/pharmacology, Bacterial Proteins/analysis, Cell-Free System, Chromatography, *Clostridium perfringens/immunology, Electrophoresis, Disc, Electrophoresis, Polyacrylamide Gel, *Enterotoxins/analysis/biosynthesis/isolation & purification/toxicity, Erythema/chemically induced, Guinea Pigs, Humans, Ileum/drug effects, Immunodiffusion, Immunoelectrophoresis, Mice, Neutralization Tests, Rabbits/immunology, Spores, BacterialPathogenicity
Metabolism4350345A paracrystalline inclusion formed during sporulation of enterotoxin-producing strains of Clostridium perfringens type A.Duncan CL, King GJ, Frieben WRJ Bacteriol10.1128/jb.114.2.845-859.19731973Clostridium/growth & development, Clostridium perfringens/*cytology/metabolism, Crystallography, Enterotoxins/*biosynthesis, Genes, *Inclusion Bodies, Microscopy, Electron, Microscopy, Phase-Contrast, Mutation, Serotyping, Spores/*growth & development, Spores, Bacterial/cytology/growth & developmentPathogenicity
Pathogenicity4353692Assay methods for Clostridium perfringens type A enterotoxin.Genigeorgis C, Sakaguchi G, Riemann HAppl Microbiol10.1128/am.26.1.111-115.19731973Animals, Clostridium perfringens/growth & development/*metabolism, Enterotoxins/*analysis/biosynthesis/isolation & purification/toxicity, Evaluation Studies as Topic, Guinea Pigs, Hemagglutination Tests, Immune Sera, Immunodiffusion, Intestines/drug effects/microbiology, Male, Methods, Mice, Neutralization Tests, Rabbits/immunology, Skin Tests, Spores, Bacterial/growth & developmentMetabolism
Stress4356461In vitro production of Clostridium perfringens enterotoxin and its detection by reversed passive hemagglutination.Uemura T, Sakaguchi G, Riemann HPAppl Microbiol10.1128/am.26.3.381-385.19731973Bacteriological Techniques, Clostridium perfringens/analysis/growth & development/*metabolism, Enterotoxins/analysis/*biosynthesis, Evaluation Studies as Topic, Hemagglutination Tests/*standards, Hot Temperature, Immunodiffusion, Methods, Species Specificity, Spores, Bacterial/growth & development, Time Factors, VibrationPhylogeny
Cultivation6257363Ultrastructure of sporulating cells of Clostridium perfringens type A grown in the presence of raffinose.Labbe RG, Rufner RCan J Microbiol10.1139/m80-1901980Clostridium perfringens/*physiology/ultrastructure, Culture Media, Microscopy, Electron, Oligosaccharides/*pharmacology, Raffinose/*pharmacology, Spores, Bacterial/ultrastructure
Cultivation6261681Effects of pH shifts, bile salts, and glucose on sporulation of Clostridium perfringens NCTC 8798.Hickey CS, Johnson MGAppl Environ Microbiol10.1128/aem.41.1.124-129.19811981Cholic Acids/*pharmacology, Clostridium perfringens/drug effects/*physiology, Culture Media, Deoxycholic Acid/*pharmacology, Glucose/*pharmacology, Hydrogen-Ion Concentration, Spores, Bacterial/physiology, Starch/pharmacologyPathogenicity
Cultivation6316852Influence of carbohydrates on growth and sporulation of Clostridium perfringens in a defined medium with or without guanosine.Sacks LEAppl Environ Microbiol10.1128/aem.46.5.1169-1175.19831983Caffeine/pharmacology, Carbohydrates/*pharmacology, Clostridium perfringens/*physiology, Culture Media, Dextrins/pharmacology, Glucose/pharmacology, Guanosine/*pharmacology, Melibiose/pharmacology, Raffinose/pharmacology, Spores, Bacterial/physiology, Sucrose/pharmacology, Theophylline/pharmacology
8868239A comparative study on the conditions of growth and sporulation of three strains of Clostridium perfringens type A.Decaudin M, Tholozan JLCan J Microbiol10.1139/m96-0441996Clostridium perfringens/*growth & development
Pathogenicity9516540Comparison of different methods of cell lysis and protein measurements in Clostridium perfringens: application to the cell volume determination.Guerlava P, Izac V, Tholozan JLCurr Microbiol10.1007/pl000067561998Bacterial Proteins/*analysis/drug effects, *Bacteriolysis, Carbon Radioisotopes/analysis, Cell-Free System/drug effects, Clostridium perfringens/*chemistry/*cytology/drug effects, Cytoplasm/chemistry/drug effects/microbiology, Cytosol/chemistry/drug effects/microbiology, Dextrans/analysis/pharmacokinetics, Endopeptidases/pharmacology, Oils/pharmacology, Sodium Dodecyl Sulfate/pharmacology, Sodium Hydroxide/pharmacology, Solubility, Sonication, Sorbitol/analysis/pharmacokineticsEnzymology
Cultivation10063637A new growth and in vitro sporulation medium for Clostridium perfringens.Meyer M, Tholozan JLLett Appl Microbiol10.1046/j.1365-2672.1999.00494.x1999Bacteriological Techniques, Biomass, Clostridium perfringens/*growth & development/physiology, *Culture Media/chemistry, Spores, Bacterial/physiology
Enzymology10419949Cloning, sequence, and transcriptional regulation of the operon encoding a putative N-acetylmannosamine-6-phosphate epimerase (nanE) and sialic acid lyase (nanA) in Clostridium perfringens.Walters DM, Stirewalt VL, Melville SBJ Bacteriol10.1128/JB.181.15.4526-4532.19991999*Bacterial Proteins, Base Sequence, Carbohydrate Epimerases/*genetics/metabolism, Cloning, Molecular, Clostridium perfringens/*enzymology/*genetics, Escherichia coli, *Gene Expression Regulation, Bacterial, Gene Expression Regulation, Enzymologic, Molecular Sequence Data, *Operon, Oxo-Acid-Lyases/*genetics/metabolism, Promoter Regions, Genetic, Recombinant Proteins/metabolism, *Transcription, GeneticMetabolism
Metabolism28052992CodY Promotes Sporulation and Enterotoxin Production by Clostridium perfringens Type A Strain SM101.Li J, Freedman JC, Evans DR, McClane BAInfect Immun10.1128/IAI.00855-162017Bacterial Proteins/genetics/*metabolism, Clostridium Infections/microbiology, Clostridium perfringens/*physiology, Enterotoxins/*biosynthesis, Gene Expression Regulation, Bacterial, Genetic Complementation Test, Mutation, Spores, Bacterial/*physiology, Transcription Factors/genetics/*metabolismBiotechnology

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
41430Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106157Collection of Institut Pasteur (CIP 106157)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
95829Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID67932.1