Strain identifier

BacDive ID: 139287

Type strain: No

Species: Lactobacillus crispatus

Strain history: CIP <- 1996, JCM <- E. Lauer <- T. Mitsuoka: strain T-91, Lactobacillus acidophilus

NCBI tax ID(s): 47770 (species)

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For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 41426

BacDive-ID: 139287

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe

description: Lactobacillus crispatus CIP 105002 is a facultative anaerobe bacterium that was isolated from Chicken feces.

NCBI tax id

  • NCBI tax id: 47770
  • Matching level: species

strain history

@refhistory
67770E. Lauer <-- T. Mitsuoka T-91.
41426CIP <- 1996, JCM <- E. Lauer <- T. Mitsuoka: strain T-91, Lactobacillus acidophilus

doi: 10.13145/bacdive139287.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Lactobacillus
  • species: Lactobacillus crispatus
  • full scientific name: Lactobacillus crispatus (Brygoo and Aladame 1953) Moore and Holdeman 1970 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Eubacterium crispatum

@ref: 41426

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Lactobacillus

species: Lactobacillus crispatus

type strain: no

Morphology

cell morphology

@refgram stainconfidencemotilitycell shape
69480positive92.045
6948092.5no
41426positivenorod-shaped

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
41426MEDIUM 40- for Lactobacillus and LeuconostocyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
41426CIP Medium 40yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40

culture temp

@refgrowthtypetemperature
41426positivegrowth37
67770positivegrowth37
41426positivegrowth30-45
41426nogrowth10
41426nogrowth15
41426nogrowth25

Physiology and metabolism

oxygen tolerance

  • @ref: 41426
  • oxygen tolerance: facultative anaerobe

spore formation

@refspore formationconfidence
69480no91.425
69481no100

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4142617632nitrate-reduction
4142616301nitrite-reduction
4142617632nitrate+respiration

metabolite tests

  • @ref: 41426
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
41426oxidase-
41426alcohol dehydrogenase-1.1.1.1
41426catalase-1.11.1.6
41426lysine decarboxylase-4.1.1.18
41426ornithine decarboxylase-4.1.1.17
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase-3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
41426--+-------++++-++---

Isolation, sampling and environmental information

isolation

@refsample type
67770Chicken feces
41426Chicken, feces

Safety information

risk assessment

  • @ref: 41426
  • biosafety level: 1
  • biosafety level comment: Risk group (French classification)

Sequence information

16S sequences

  • @ref: 124043
  • description: Lactobacillus crispatus gene for 16S rRNA, partial sequence, strain: JCM 5810.
  • accession: AB289071
  • length: 675
  • database: nuccore
  • NCBI tax ID: 47770

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Lactobacillus crispatus JCM 5810GCA_001567095contigncbi47770
66792Lactobacillus crispatus strain JCM 581047770.37wgspatric47770
66792Lactobacillus crispatus JCM 58102690315764draftimg47770

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69481spore-formingspore-formingAbility to form endo- or exosporesno100no
69480gram-positivegram-positivePositive reaction to Gram-stainingyes92.045no
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no70.144no
69480spore-formingspore-formingAbility to form endo- or exosporesno91.425no
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)no96.69yes
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno96.5yes
69480flagellatedmotile2+Ability to perform flagellated movementno92.5no

External links

@ref: 41426

culture collection no.: CIP 105002, JCM 5810, KCTC 3178, LMG 18191

straininfo link

  • @ref: 95827
  • straininfo: 11442

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Metabolism11053389Characterization of the collagen-binding S-layer protein CbsA of Lactobacillus crispatus.Sillanpaa J, Martinez B, Antikainen J, Toba T, Kalkkinen N, Tankka S, Lounatmaa K, Keranen J, Hook M, Westerlund-Wikstrom B, Pouwels PH, Korhonen TKJ Bacteriol10.1128/JB.182.22.6440-6450.20002000Amino Acid Sequence, Animals, Artificial Gene Fusion, Bacterial Proteins/genetics/*metabolism, Chickens, Cloning, Molecular, Collagen/*metabolism, Genes, Bacterial, Lactobacillus/chemistry/*genetics, *Membrane Glycoproteins, Membrane Proteins/genetics/*metabolism, Molecular Sequence Data, Point MutationEnzymology
Metabolism11073938Expression of cbsA encoding the collagen-binding S-protein of Lactobacillus crispatus JCM5810 in Lactobacillus casei ATCC 393(T).Martinez B, Sillanpaa J, Smit E, Korhonen TK, Pouwels PHJ Bacteriol10.1128/JB.182.23.6857-6861.20002000Bacterial Proteins/*biosynthesis/genetics, Cell Membrane/metabolism, Integrins/*biosynthesis/genetics, Lactobacillus/*genetics, Lactobacillus casei/genetics/*metabolism, *Membrane Glycoproteins, Membrane Proteins/*biosynthesis/genetics, Receptors, Collagen
Enzymology11872114Inhibition of the adherence of Escherichia coli strains to basement membrane by Lactobacillus crispatus expressing an S-layer.Horie M, Ishiyama A, Fujihira-Ueki Y, Sillanpaa J, Korhonen TK, Toba TJ Appl Microbiol10.1046/j.1365-2672.2002.01539.x2002*Antibiosis, *Bacterial Adhesion/drug effects/physiology, Bacterial Proteins/isolation & purification/pharmacology, Basement Membrane/chemistry/*microbiology, Collagen, Drug Combinations, Escherichia coli/growth & development/*physiology, Lactobacillus/*growth & development/metabolism, Laminin, *Membrane Glycoproteins, Membrane Proteins/isolation & purification/pharmacology, ProteoglycansPhylogeny
Metabolism12406216Domains in the S-layer protein CbsA of Lactobacillus crispatus involved in adherence to collagens, laminin and lipoteichoic acids and in self-assembly.Antikainen J, Anton L, Sillanpaa J, Korhonen TKMol Microbiol10.1046/j.1365-2958.2002.03180.x2002*Bacterial Adhesion, Bacterial Proteins/*chemistry/genetics/metabolism, Cell Wall/metabolism, Collagen/*metabolism, Gene Deletion, Lactobacillus/genetics/*metabolism/physiology, Lactobacillus casei/genetics/metabolism, Laminin/*metabolism, Lipopolysaccharides/*metabolism, *Membrane Glycoproteins, Membrane Proteins/*chemistry/genetics/metabolism, Microscopy, Electron, Molecular Sequence Data, Sequence Analysis, DNA, Teichoic Acids/*metabolismGenetics
16535065A Collagen-Binding S-Layer Protein in Lactobacillus crispatus.Toba T, Virkola R, Westerlund B, Bjorkman Y, Sillanpaa J, Vartio T, Kalkkinen N, Korhonen TKAppl Environ Microbiol10.1128/aem.61.7.2467-2471.19951995
Genetics27081134Draft Genome Sequence of Lactobacillus crispatus JCM5810, Which Can Reduce Campylobacter jejuni Colonization in Chicken Intestine.Wooten J, Liu X, Miller MJGenome Announc10.1128/genomeA.00255-162016
Metabolism32045605S-layer protein 2 of Lactobacillus crispatus 2029, its structural and immunomodulatory characteristics and roles in protective potential of the whole bacteria against foodborne pathogens.Abramov VM, Kosarev IV, Priputnevich TV, Machulin AV, Khlebnikov VS, Pchelintsev SY, Vasilenko RN, Sakulin VK, Suzina NE, Chikileva IO, Derysheva EI, Melnikov VG, Nikonov IN, Samoilenko VA, Svetoch EE, Sukhikh GT, Uversky VN, Karlyshev AVInt J Biol Macromol10.1016/j.ijbiomac.2020.02.0652020*Antibiosis, Bacterial Adhesion, Bacterial Outer Membrane Proteins/chemistry/immunology/metabolism, Bile Acids and Salts, Caspase 3/metabolism, Caspase 9/metabolism, Cell Line, Cell Survival, Epithelial Cells, Foodborne Diseases/*diet therapy/*microbiology, *Immunomodulation, Inflammation Mediators/metabolism, Intestinal Mucosa/metabolism/microbiology, Lactobacillus crispatus/*physiology, Membrane Glycoproteins/*chemistry/*immunology, *Probiotics, Stress, Physiological, Structure-Activity RelationshipPathogenicity

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
41426Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105002Collection of Institut Pasteur (CIP 105002)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
95827Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID11442.1
124043Dr. Isabel Schober, Dr. Julia KoblitzData extracted from sequence databases, automatically matched based on designation and taxonomy