Strain identifier

BacDive ID: 139286

Type strain: No

Species: Lactobacillus crispatus

Strain Designation: Sp34

Strain history: CIP <- 1996, JCM <- E. Lauer, Lactobacillus acidophilus <- G. Reuter: strain Sp34

NCBI tax ID(s): 47770 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 8.1 (current version)

General

@ref: 41425

BacDive-ID: 139286

keywords: Bacteria, facultative anaerobe, mesophilic, Gram-positive, motile, rod-shaped

description: Lactobacillus crispatus Sp34 is a facultative anaerobe, mesophilic, Gram-positive bacterium that was isolated from Sputum .

NCBI tax id

  • NCBI tax id: 47770
  • Matching level: species

strain history

@refhistory
67770E. Lauer <-- G. Reuter Sp34.
41425CIP <- 1996, JCM <- E. Lauer, Lactobacillus acidophilus <- G. Reuter: strain Sp34

doi: 10.13145/bacdive139286.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Lactobacillus
  • species: Lactobacillus crispatus
  • full scientific name: Lactobacillus crispatus (Brygoo and Aladame 1953) Moore and Holdeman 1970 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Eubacterium crispatum

@ref: 41425

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Lactobacillus

species: Lactobacillus crispatus

strain designation: Sp34

type strain: no

Morphology

cell morphology

  • @ref: 41425
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: yes

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
41425MEDIUM 40- for Lactobacillus and LeuconostocyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
41425CIP Medium 40yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40

culture temp

@refgrowthtypetemperaturerange
41425positivegrowth37mesophilic
67770positivegrowth37mesophilic
41425positivegrowth22-37
41425nogrowth10psychrophilic
41425nogrowth15psychrophilic
41425nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 41425
  • oxygen tolerance: facultative anaerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4142517632nitrate-reduction
4142516301nitrite-reduction
4142517632nitrate+respiration

antibiotic resistance

  • @ref: 41425
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite tests

  • @ref: 41425
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
41425oxidase-
41425alcohol dehydrogenase-1.1.1.1
41425catalase-1.11.1.6
41425lysine decarboxylase-4.1.1.18
41425ornithine decarboxylase-4.1.1.17
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase+3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
41425--++-+-+--++++-++---

Isolation, sampling and environmental information

isolation

@refsample type
67770Sputum (adult)
41425Human, Adult

Safety information

risk assessment

  • @ref: 41425
  • biosafety level: 1
  • biosafety level comment: Risk group (French classification)

Sequence information

GC content

  • @ref: 67770
  • GC-content: 33.4
  • method: thermal denaturation, midpoint method (Tm)

External links

@ref: 41425

culture collection no.: CIP 105001, JCM 5808, KCTC 3176, LMG 18189

straininfo link

  • @ref: 95826
  • straininfo: 125703

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
41425Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105001Collection of Institut Pasteur (CIP 105001)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
95826Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID125703.1