Strain identifier

BacDive ID: 139250

Type strain: No

Species: Nocardia pseudobrasiliensis

Strain Designation: N 249

Strain history: CIP <- 1995, R. Ruimy, CNRS, Villefranche/Mer, France: strain N 249 <- R.J. Wallace, Texas, USA

NCBI tax ID(s): 45979 (species)

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General

@ref: 41380

BacDive-ID: 139250

keywords: Bacteria, obligate aerobe, mesophilic, Gram-positive, rod-shaped

description: Nocardia pseudobrasiliensis N 249 is an obligate aerobe, mesophilic, Gram-positive bacterium of the family Nocardiaceae.

NCBI tax id

  • NCBI tax id: 45979
  • Matching level: species

strain history

  • @ref: 41380
  • history: CIP <- 1995, R. Ruimy, CNRS, Villefranche/Mer, France: strain N 249 <- R.J. Wallace, Texas, USA

doi: 10.13145/bacdive139250.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Nocardiaceae
  • genus: Nocardia
  • species: Nocardia pseudobrasiliensis
  • full scientific name: Nocardia pseudobrasiliensis Ruimy et al. 1996

@ref: 41380

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Mycobacteriales

family: Nocardiaceae

genus: Nocardia

species: Nocardia pseudobrasiliensis

strain designation: N 249

type strain: no

Morphology

cell morphology

  • @ref: 41380
  • gram stain: positive
  • cell shape: rod-shaped

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
41380MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
41380CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
41380positivegrowth30mesophilic
41380positivegrowth37mesophilic
41380nogrowth10psychrophilic
41380nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 41380
  • oxygen tolerance: obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
41380NaClpositivegrowth0-4 %
41380NaClnogrowth6 %
41380NaClnogrowth8 %
41380NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
413804853esculin-hydrolysis
41380606565hippurate-hydrolysis
4138017632nitrate+reduction
4138016301nitrite-reduction
4138017632nitrate-respiration

metabolite production

  • @ref: 41380
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 41380
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: +

enzymes

@refvalueactivityec
41380oxidase-
41380beta-galactosidase-3.2.1.23
41380alcohol dehydrogenase-1.1.1.1
41380gelatinase+
41380amylase-
41380DNase+
41380caseinase+3.4.21.50
41380catalase+1.11.1.6
41380tween esterase-
41380lecithinase-
41380lysine decarboxylase-4.1.1.18
41380ornithine decarboxylase-4.1.1.17
41380urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase+3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase+3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
41380-+++-+++--++-+-++++-

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
41380++++--------------++-----------++--------------+-+----+----+------------+--+---++-------+++--++--++

Isolation, sampling and environmental information

isolation

@refgeographic locationcountryorigin.countrycontinentsample type
41380TexasUSAUSANorth America
41380TexasUnited States of AmericaUSANorth AmericaHuman, Brain abscess

Safety information

risk assessment

  • @ref: 41380
  • biosafety level: 2
  • biosafety level comment: Risk group (French classification)

External links

@ref: 41380

culture collection no.: CIP 104601

straininfo link

  • @ref: 95797
  • straininfo: 69525

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
41380Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104601Collection of Institut Pasteur (CIP 104601)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar SchomburgBRENDA, the ELIXIR core data resource in 2021: new developments and updates10.1093/nar/gkaa1025Nucleic Acids Res. 49: D498-D508 202033211880
68382Automatically annotated from API zym
95797Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID69525.1