Strain identifier

BacDive ID: 1392

Type strain: Yes

Species: Thalassobacillus cyri

Strain Designation: HS286

Strain history: A. Ventosa HS286.

NCBI tax ID(s): 571932 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 15851

BacDive-ID: 1392

DSM-Number: 21635

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, moderately halophilic, spore-forming, mesophilic, Gram-positive, motile, rod-shaped, colony-forming

description: Thalassobacillus cyri HS286 is an obligate aerobe, moderately halophilic, spore-forming bacterium that forms circular colonies and was isolated from brine.

NCBI tax id

  • NCBI tax id: 571932
  • Matching level: species

strain history

@refhistory
15851<- C. Sanchez-Porro, Univ. Seville, Dept. Microbiol. Parasitol., Spain; HS286 <- R. Rohban and M. A. Amoozegar
67770A. Ventosa HS286.

doi: 10.13145/bacdive1392.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Thalassobacillus
  • species: Thalassobacillus cyri
  • full scientific name: Thalassobacillus cyri Sánchez-Porro et al. 2009

@ref: 15851

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Thalassobacillus

species: Thalassobacillus cyri

full scientific name: Thalassobacillus cyri Sánchez-Porro et al. 2009

strain designation: HS286

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
23022positive1.3-8.5 µm0.6-0.8 µmrod-shapedyes
69480yes98.274
69480positive100

colony morphology

  • @ref: 23022
  • colony size: 2.0 mm
  • colony color: cream-beige
  • colony shape: circular
  • incubation period: 2 days
  • medium used: 10 % HM agar medium

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
15851THALASSOBACILLUS CYRI MEDIUM (DSMZ Medium 1213)yeshttps://mediadive.dsmz.de/medium/1213Name: THALASSOBACILLUS CYRI MEDIUM (DSMZ Medium 1213) Composition: NaCl 78.0 g/l MgSO4 x 7 H2O 20.3 g/l Agar 15.0 g/l MgCl2 x 6 H2O 13.0 g/l Yeast extract 5.0 g/l KCl 2.0 g/l CaCl2 x 2 H2O 0.33 g/l NaBr 0.23 g/l NaHCO3 0.06 g/l Distilled water
2302210 % HM agar mediumyes

culture temp

@refgrowthtypetemperaturerange
15851positivegrowth35mesophilic
23022positivegrowth5.0-45.0
23022positiveoptimum40.0thermophilic
67770positivegrowth34mesophilic

culture pH

@refabilitytypepHPH range
23022positivegrowth7.0-9.5alkaliphile
23022positiveoptimum7.5

Physiology and metabolism

oxygen tolerance

  • @ref: 23022
  • oxygen tolerance: obligate aerobe

spore formation

@refspore descriptiontype of sporespore formationconfidence
23022ellipsoidal,central-subterminal,in swollen sporangiaendosporeyes
69481yes100
69480yes100

halophily

@refhalophily levelsaltgrowthtested relationconcentration
23022moderately halophilic
23022NaClpositivegrowth1.0-15.0 %(w/v)
23022NaCloptimum8.0 %(w/v)

murein

  • @ref: 23022
  • murein short key: A31
  • type: A1gamma m-Dpm-direct

observation

  • @ref: 67770
  • observation: quinones: MK-7

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2302265327D-xylose-builds acid from
2302217716lactose-builds acid from
2302217268myo-inositol-builds acid from
230225291gelatin-hydrolysis
2302216301nitrite-reduction
2302215824D-fructose+builds acid from
2302217634D-glucose+builds acid from
2302216024D-mannose+builds acid from
2302228260galactose+builds acid from
2302217306maltose+builds acid from
2302228053melibiose+builds acid from
2302227082trehalose+builds acid from
2302217234glucose+fermentation
23022casein+hydrolysis
2302216991dna+hydrolysis
230224853esculin+hydrolysis
2302228017starch+hydrolysis
2302253426tween 80+hydrolysis
2302217632nitrate+reduction

antibiotic resistance

@refChEBImetaboliteis antibioticis resistantresistance conc.is sensitivesensitivity conc.
2302228864tobramycinyesyes10 µg
230226104kanamycinyesyes30 µg
230228309polymyxin byesyes100 Unit
230223393carbenicillinyesyes100 µg
230222676amoxicillinyesyes30 µg
2302217833gentamicinyesyes30 µg
2302227902tetracyclineyesyes30 µg
2302271415nitrofurantoinyesyes300 µg
2302228077rifampicinyesyes5 µg

metabolite production

@refChebi-IDmetaboliteproduction
2302235581indoleno
2302216136hydrogen sulfideno
2302215688acetoinno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
2302215688acetoin-
2302217234glucose+

enzymes

@refvalueactivityec
23022arginine dihydrolase+3.5.3.6
23022beta-galactosidase-3.2.1.23
23022catalase+1.11.1.6
23022cytochrome oxidase+1.9.3.1
23022lysine decarboxylase+4.1.1.18
23022ornithine decarboxylase-4.1.1.17
23022phenylalanine deaminase-4.3.1.5
23022urease-3.5.1.5

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentenrichment cultureenrichment culture compositionenrichment culture temperature
15851brineHowz Soltan LakeIranIRNAsia
23022water of the hypersaline lake10 % HM mediumcontaining (l-1): 81 g NaCl, 7 g MgCl2 , 9.6 g MgSO4 , 0.36 g CaCl2 , 2 g KCl, 0.06 g NaHCO3 , 0.026 g NaBr, 5 g proteose-peptone no. 3 (Difco); 10 g yeast extract (Difco) and 1 g glucose37.0
67770Water from the hypersaline lake Howz-Soltan in Iran

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Lake (large)
#Condition#Saline

Safety information

risk assessment

  • @ref: 15851
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 15851
  • description: Thalassobacillus cyri partial 16S rRNA gene, type strain HS286T
  • accession: FM864226
  • length: 1470
  • database: ena
  • NCBI tax ID: 571932

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Thalassobacillus cyri strain CCM7597571932.3wgspatric571932
66792Thalassobacillus cyri CCM75972675903014draftimg571932
67770Thalassobacillus cyri CCM7597GCA_900107755scaffoldncbi571932

GC content

@refGC-contentmethod
1585143.0thermal denaturation, midpoint method (Tm)
6777043thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
gram-positiveyes83.703no
anaerobicno98.568no
halophileyes73.539no
spore-formingyes92.461yes
glucose-utilyes91.033no
flagellatedyes75.002no
aerobicyes93.408no
thermophileno93.084no
motileyes86.948no
glucose-fermentno89.374no

External links

@ref: 15851

culture collection no.: DSM 21635, CCM 7597, JCM 15722

straininfo link

  • @ref: 71043
  • straininfo: 403649

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny19622638Thalassobacillus cyri sp. nov., a moderately halophilic Gram-positive bacterium from a hypersaline lake.Sanchez-Porro C, Amoozegar MA, Rohban R, Hajighasemi M, Ventosa AInt J Syst Evol Microbiol10.1099/ijs.0.010488-02009Bacillaceae/classification/genetics/*isolation & purification/*metabolism, Base Composition, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/chemistry/metabolism, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sodium Chloride/*metabolism, *Water MicrobiologyMetabolism
Phylogeny19854876Thalassobacillus hwangdonensis sp. nov., isolated from a tidal flat sediment.Lee SY, Oh TK, Yoon JHInt J Syst Evol Microbiol10.1099/ijs.0.016204-02009Bacillaceae/*classification/genetics/*isolation & purification/metabolism, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/metabolism, Geologic Sediments/*microbiology, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiologyMetabolism
Phylogeny20562240Thalassobacillus pellis sp. nov., a moderately halophilic, Gram-positive bacterium isolated from salted hides.Sanchez-Porro C, Yilmaz P, de la Haba RR, Birbir M, Ventosa AInt J Syst Evol Microbiol10.1099/ijs.0.024778-02010Bacillaceae/*classification/genetics/*isolation & purification/metabolism, DNA, Bacterial/genetics, Fatty Acids/metabolism, Geologic Sediments/*microbiology, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sodium Chloride/*metabolismMetabolism

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
15851Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21635)https://www.dsmz.de/collection/catalogue/details/culture/DSM-21635
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
23022C. Sanchez-Porro,M. A. Amoozegar,R. Rohban,M. Hajighasemi,A. Ventosa10.1099/ijs.0.010488-0Thalassobacillus cyri sp. nov., a moderately halophilic Gram-positive bacterium from a hypersaline lakeIJSEM 59: 2565-2570 200919622638
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
71043Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID403649.1StrainInfo: A central database for resolving microbial strain identifiers