Strain identifier

BacDive ID: 13917

Type strain: Yes

Species: Thauera aminoaromatica

Strain Designation: S2

Strain history: CIP <- 2010, DSMZ <- G. Fuchs, Freiburg Univ., Freiburg, Germany <- E. Lang, DSMZ, Braunschweig, Germany: strain S2

NCBI tax ID(s): 164330 (species)

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General

@ref: 5437

BacDive-ID: 13917

DSM-Number: 14742

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Thauera aminoaromatica S2 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from anaerobic sawage sludge.

NCBI tax id

  • NCBI tax id: 164330
  • Matching level: species

strain history

@refhistory
5437<- G. Fuchs; S2
116331CIP <- 2010, DSMZ <- G. Fuchs, Freiburg Univ., Freiburg, Germany <- E. Lang, DSMZ, Braunschweig, Germany: strain S2

doi: 10.13145/bacdive13917.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Rhodocyclales
  • family: Zoogloeaceae
  • genus: Thauera
  • species: Thauera aminoaromatica
  • full scientific name: Thauera aminoaromatica Mechichi et al. 2002

@ref: 5437

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Rhizobiales

family: Zoogloeaceae

genus: Thauera

species: Thauera aminoaromatica

full scientific name: Thauera aminoaromatica Mechichi et al. 2002

strain designation: S2

type strain: yes

Morphology

cell morphology

  • @ref: 116331
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5437REACTIVATION WITH LIQUID MEDIUM 830 (DSMZ Medium 830c)yeshttps://mediadive.dsmz.de/medium/830cName: REACTIVATION WITH LIQUID MEDIUM 830 (DSMZ Medium 830c) Composition: Agar 15.0 g/l Yeast extract 0.5 g/l Proteose peptone 0.5 g/l Casamino acids 0.5 g/l Glucose 0.5 g/l Starch 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
5437THAUERA AROMATICA MEDIUM (DSMZ Medium 586)yeshttps://mediadive.dsmz.de/medium/586Name: THAUERA AROMATICA MEDIUM (DSMZ Medium 586) Composition: K2HPO4 5.83251 g/l KNO3 1.97044 g/l KH2PO4 0.803941 g/l Na-benzoate 0.70936 g/l NH4Cl 0.522168 g/l MgSO4 x 7 H2O 0.197044 g/l CaCl2 x 2 H2O 0.0246305 g/l HCl 0.0246305 g/l FeCl2 x 4 H2O 0.0147783 g/l CoCl2 x 6 H2O 0.00187192 g/l MnCl2 x 4 H2O 0.000985222 g/l ZnCl2 0.000689655 g/l Na2MoO4 x 2 H2O 0.00035468 g/l p-Aminobenzoic acid 0.000246305 g/l alpha-lipoic acid 0.000246305 g/l Riboflavin 0.000246305 g/l Pantothenic acid 0.000246305 g/l Vitamin B12 0.000246305 g/l Thiamine-HCl x 2 H2O 0.000246305 g/l NiCl2 x 6 H2O 0.000236453 g/l Nicotine amide 0.000123153 g/l Nicotinic acid 0.000123153 g/l Folic acid 9.85222e-05 g/l Biotin 9.85222e-05 g/l H3BO3 5.91133e-05 g/l Pyridoxamine hydrochloride 4.92611e-05 g/l CuCl2 x 2 H2O 1.97044e-05 g/l Distilled water
40651MEDIUM 566- Reasoner's 2A agar for Flavobacterium micromatiyesDistilled water make up to (1000.000 ml);R2A agar (18.200 g)
116331CIP Medium 566yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=566

culture temp

@refgrowthtypetemperaturerange
5437positivegrowth30mesophilic
40651positivegrowth30mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
5437aerobe
5437anaerobe
116331obligate aerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
11633117632nitrate+reduction
11633116301nitrite+reduction

metabolite production

  • @ref: 116331
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
116331oxidase+
116331catalase+1.11.1.6
116331urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
116331-+++-+----++--------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
5437anaerobic sawage sludgeKonstanzGermanyDEUEurope
116331Environment, Sewage sludgeGermanyDEUEurope1991

isolation source categories

Cat1Cat2Cat3
#Engineered#Waste#Sewage sludge
#Condition#Anoxic (anaerobic)

taxonmaps

  • @ref: 69479
  • File name: preview.99_1902.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_129;96_594;97_695;98_810;99_1902&stattab=map
  • Last taxonomy: Thauera
  • 16S sequence: AJ315677
  • Sequence Identity:
  • Total samples: 548
  • soil counts: 20
  • aquatic counts: 491
  • animal counts: 31
  • plant counts: 6

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
54371Risk group (German classification)
1163311Risk group (French classification)

Sequence information

16S sequences

  • @ref: 5437
  • description: Thauera sp. S2 16S rRNA gene, strain S2
  • accession: AJ315677
  • length: 1496
  • database: ena
  • NCBI tax ID: 1234381

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Thauera aminoaromatica S2GCA_000310185contigncbi1234381
66792Thauera aminoaromatica S21234381.3wgspatric1234381
66792Thauera aminoaromatica S22537561694draftimg1234381

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes90.822no
gram-positiveno99.241no
anaerobicno90.792no
aerobicno67.756no
halophileno92.074no
spore-formingno94.161no
glucose-utilno73.913no
flagellatedyes91.153no
thermophileno94.822yes
glucose-fermentno87.107no

External links

@ref: 5437

culture collection no.: DSM 14742, LMG 26917, CIP 110164

straininfo link

  • @ref: 83092
  • straininfo: 87977

literature

  • topic: Phylogeny
  • Pubmed-ID: 29185938
  • title: Thauera propionica sp. nov., isolated from downstream sediment sample of the river Ganges, Kanpur, India.
  • authors: Pal D, Bhardwaj A, Sudan SK, Kaur N, Kumari M, Bisht B, Vyas B, Krishnamurthi S, Mayilraj S
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002508
  • year: 2017
  • mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Geologic Sediments/*microbiology, India, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Rivers/*microbiology, Sequence Analysis, DNA, Thauera/*classification/genetics/isolation & purification, Ubiquinone/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorscataloguedoi/urltitle
5437Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14742)https://www.dsmz.de/collection/catalogue/details/culture/DSM-14742
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
40651Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/7993
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
83092Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID87977.1StrainInfo: A central database for resolving microbial strain identifiers
116331Curators of the CIPCollection of Institut Pasteur (CIP 110164)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20110164