Strain identifier

BacDive ID: 13916

Type strain: Yes

Species: Thauera mechernichensis

Strain Designation: TL1

Strain history: CIP <- 2001, DSMZ <- H. Diekmann, Hannover Univ., Hannover, Germany: TL1

NCBI tax ID(s): 82788 (species)

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General

@ref: 4664

BacDive-ID: 13916

DSM-Number: 12266

keywords: genome sequence, Bacteria, aerobe, mesophilic, Gram-negative, rod-shaped

description: Thauera mechernichensis TL1 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from sewage treatment plant.

NCBI tax id

  • NCBI tax id: 82788
  • Matching level: species

strain history

@refhistory
4664<- H. Diekmann, Univ. Hannover; TL1
120866CIP <- 2001, DSMZ <- H. Diekmann, Hannover Univ., Hannover, Germany: TL1

doi: 10.13145/bacdive13916.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Rhodocyclales
  • family: Zoogloeaceae
  • genus: Thauera
  • species: Thauera mechernichensis
  • full scientific name: Thauera mechernichensis Scholten et al. 1999

@ref: 4664

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Rhizobiales

family: Zoogloeaceae

genus: Thauera

species: Thauera mechernichensis

full scientific name: Thauera mechernichensis Scholten et al. 1999

strain designation: TL1

type strain: yes

Morphology

cell morphology

  • @ref: 120866
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 120866
  • hemolysis ability: 1

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
4664REACTIVATION WITH LIQUID MEDIUM 830 (DSMZ Medium 830c)yeshttps://mediadive.dsmz.de/medium/830cName: REACTIVATION WITH LIQUID MEDIUM 830 (DSMZ Medium 830c) Composition: Agar 15.0 g/l Yeast extract 0.5 g/l Proteose peptone 0.5 g/l Casamino acids 0.5 g/l Glucose 0.5 g/l Starch 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
4664THAUERA MECHERNICHI MEDIUM (DSMZ Medium 918)yeshttps://mediadive.dsmz.de/medium/918Name: THAUERA MECHERNICHI MEDIUM (DSMZ Medium 918) Composition: Na2HPO4 4.2 g/l Na-acetate 2.93 g/l KH2PO4 1.5 g/l NH4Cl 0.3 g/l MgSO4 x 7 H2O 0.1 g/l Na2-EDTA 0.1 g/l CaCl2 x 2 H2O 0.011 g/l MnCl2 x 4 H2O 0.01012 g/l FeSO4 x 7 H2O 0.01 g/l ZnSO4 x 7 H2O 0.004 g/l CoCl2 x 6 H2O 0.00322 g/l CuSO4 x 5 H2O 0.00314 g/l (NH4)6Mo7O24 x 4 H2O 0.0022 g/l Distilled water
39622MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
120866CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
4664positivegrowth37mesophilic
39622positivegrowth37mesophilic
58381positivegrowth22-42
120866positivegrowth15-45
120866nogrowth5psychrophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
58381aerobe
120866facultative anaerobe

halophily

  • @ref: 120866
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1208664853esculin+hydrolysis
120866606565hippurate+hydrolysis
12086617632nitrate-builds gas from
12086617632nitrate+reduction
12086616301nitrite+builds gas from
12086616301nitrite+reduction
12086615792malonate-assimilation
12086617632nitrate+respiration

antibiotic resistance

  • @ref: 120866
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 120866
  • Chebi-ID: 35581
  • metabolite: indole
  • production: yes

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12086615688acetoin-
12086617234glucose-

enzymes

@refvalueactivityec
120866oxidase+
120866beta-galactosidase+3.2.1.23
120866alcohol dehydrogenase-1.1.1.1
120866gelatinase-
120866amylase-
120866DNase-
120866caseinase-3.4.21.50
120866catalase+1.11.1.6
120866tween esterase+
120866gamma-glutamyltransferase+2.3.2.2
120866lecithinase-
120866lipase-
120866lysine decarboxylase+4.1.1.18
120866ornithine decarboxylase+4.1.1.17
120866phenylalanine ammonia-lyase-4.3.1.24
120866tryptophan deaminase-
120866urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)-
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase+3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120866-+---+----+++++-----

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
120866+++++-+++++++++--+-+++-++----+-+-+--+-+----++--+++----++---++-+--+---+-----+---++-+---+--+-++++---+

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
4664sewage treatment plantMechernich, near AachenGermanyDEUEurope
58381Sewage treatment plantGermanyDEUEurope
120866Environment, Sewage treatment plantGermanyDEUEurope

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Waste
  • Cat3: #Water treatment plant

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
46641Risk group (German classification)
1208661Risk group (French classification)

Sequence information

Genome sequences

  • @ref: 66792
  • description: Thauera mechernichensis TL1
  • accession: GCA_029588855
  • assembly level: scaffold
  • database: ncbi
  • NCBI tax ID: 82788

GC content

  • @ref: 4664
  • GC-content: 65

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes88.558no
flagellatedyes66.468no
gram-positiveno97.866no
anaerobicno97.029no
aerobicyes60.708no
halophileno85.754no
spore-formingno94.966no
glucose-fermentno83.2no
thermophileno94.376yes
glucose-utilno73.489no

External links

@ref: 4664

culture collection no.: CCUG 48884, CIP 106978, ATCC 700857, DSM 12266

straininfo link

  • @ref: 83091
  • straininfo: 100996

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny10425762Thauera mechernichensis sp. nov., an aerobic denitrifier from a leachate treatment plant.Scholten E, Lukow T, Auling G, Kroppenstedt RM, Rainey FA, Diekmann HInt J Syst Bacteriol10.1099/00207713-49-3-10451999Aerobiosis, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Gram-Negative Aerobic Rods and Cocci/*classification/isolation & purification/*physiology, Molecular Sequence Data, Nitrate Reductase, Nitrate Reductases/metabolism, Nitrates/*metabolism, Nucleic Acid Hybridization, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, *Refuse Disposal, Sequence Analysis, DNAEnzymology
Phylogeny29185938Thauera propionica sp. nov., isolated from downstream sediment sample of the river Ganges, Kanpur, India.Pal D, Bhardwaj A, Sudan SK, Kaur N, Kumari M, Bisht B, Vyas B, Krishnamurthi S, Mayilraj SInt J Syst Evol Microbiol10.1099/ijsem.0.0025082017Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Geologic Sediments/*microbiology, India, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Rivers/*microbiology, Sequence Analysis, DNA, Thauera/*classification/genetics/isolation & purification, Ubiquinone/analogs & derivatives/chemistryTranscriptome

Reference

@idauthorscataloguedoi/urltitle
4664Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 12266)https://www.dsmz.de/collection/catalogue/details/culture/DSM-12266
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
39622Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/19168
58381Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 48884)https://www.ccug.se/strain?id=48884
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68382Automatically annotated from API zym
83091Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID100996.1StrainInfo: A central database for resolving microbial strain identifiers
120866Curators of the CIPCollection of Institut Pasteur (CIP 106978)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106978