Strain identifier

BacDive ID: 13915

Type strain: No

Species: Thauera terpenica

Strain Designation: 21Mol

Strain history: <- J. Harder; 21Mol

NCBI tax ID(s): 76113 (species)

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General

@ref: 4616

BacDive-ID: 13915

DSM-Number: 12140

keywords: Bacteria, aerobe, mesophilic

description: Thauera terpenica 21Mol is an aerobe, mesophilic bacterium that was isolated from activated sludge.

NCBI tax id

  • NCBI tax id: 76113
  • Matching level: species

strain history

  • @ref: 4616
  • history: <- J. Harder; 21Mol

doi: 10.13145/bacdive13915.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Rhodocyclales
  • family: Zoogloeaceae
  • genus: Thauera
  • species: Thauera terpenica
  • full scientific name: Thauera terpenica Foss and Harder 1999

@ref: 4616

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Rhizobiales

family: Zoogloeaceae

genus: Thauera

species: Thauera terpenica

full scientific name: Thauera terpenica Foss and Harder 1999

strain designation: 21Mol

type strain: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
4616ANAEROBIC THAUERA MEDIUM (DSMZ Medium 1315)yeshttps://mediadive.dsmz.de/medium/1315Name: ANAEROBIC THAUERA MEDIUM (DSMZ Medium 1315) Composition: NaHCO3 2.52 g/l Na2HPO4 x 2 H2O 1.45 g/l K-acetate 0.98 g/l KNO3 0.85 g/l MgSO4 x 7 H2O 0.5 g/l NH4Cl 0.3 g/l KH2PO4 0.25 g/l NaCl 0.05 g/l MnSO4 0.01 g/l CaCl2 0.01 g/l HCl 0.0025 g/l FeCl2 x 4 H2O 0.0015 g/l NaOH 0.001 g/l alpha-lipoic acid 0.00025 g/l Riboflavin 0.00025 g/l Thiamine-HCl x 2 H2O 0.00025 g/l Vitamin B12 0.00025 g/l Pantothenic acid 0.00025 g/l p-Aminobenzoic acid 0.00025 g/l CoCl2 x 6 H2O 0.00019 g/l Nicotinic acid 0.000125 g/l Nicotine amide 0.000125 g/l Folic acid 0.0001 g/l MnCl2 x 4 H2O 0.0001 g/l Biotin 0.0001 g/l ZnCl2 7e-05 g/l Pyridoxamine hydrochloride 5e-05 g/l Na2MoO4 x 2 H2O 3.6e-05 g/l NiCl2 x 6 H2O 2.4e-05 g/l Na2WO4 x 2 H2O 8e-06 g/l Na2SeO3 x 5 H2O 6e-06 g/l H3BO3 6e-06 g/l CuCl2 x 2 H2O 2e-06 g/l Distilled water
4616BHI MEDIUM (DSMZ Medium 215)yeshttps://mediadive.dsmz.de/medium/215Name: BHI MEDIUM (DSMZ Medium 215) Composition: Brain heart infusion 37.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
4616positivegrowth30mesophilic
55111positivegrowth30mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
4616aerobe
4616anaerobe
55111aerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate-assimilation
6836927689decanoate-assimilation
6836924265gluconate-assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose-assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate+reduction

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68369cytochrome oxidase+1.9.3.1
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase-3.1.3.2
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    55111C10:00.210
    55111C12:08.712
    55111C14:01.114
    55111C15:00.515
    55111C16:016.716
    55111C10:0 3OH7.811.423
    55111C13:0 ISO 2OH0.813.814
    55111C15:0 ISO 3OH1.616.135
    55111C16:1 ω5c0.515.908
    55111C16:1 ω7c57.115.819
    55111C18:1 ω7c /12t/9t1.717.824
    55111C18:2 ω6,9c/C18:0 ANTE0.817.724
    55111Unidentified1.916.293
    55111Unidentified0.518.305
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
55111--++-+--------------

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
55111+----------------+--+

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
4616activated sludgeGermanyDEUEurope
55111Activated sludgeGermanyDEUEurope

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Waste
  • Cat3: #Activated sludge

Safety information

risk assessment

  • @ref: 4616
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

External links

@ref: 4616

culture collection no.: DSM 12140, CCUG 41528

straininfo link

  • @ref: 83090
  • straininfo: 49241

literature

  • topic: Phylogeny
  • Pubmed-ID: 9841126
  • title: Thauera linaloolentis sp. nov. and Thauera terpenica sp. nov., isolated on oxygen-containing monoterpenes (linalool, menthol, and eucalyptol) nitrate.
  • authors: Foss S, Harder J
  • journal: Syst Appl Microbiol
  • DOI: 10.1016/s0723-2020(98)80046-5
  • year: 1998
  • mesh: Acyclic Monoterpenes, Anaerobiosis, Base Composition, Biodegradation, Environmental, Culture Media, *Cyclohexanols, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Eucalyptol, Fatty Acids/analysis, Gram-Negative Facultatively Anaerobic Rods/*classification/growth & development/isolation & purification/*metabolism, Menthol/analogs & derivatives/metabolism, *Monoterpenes, Nitrates/*metabolism, Nucleic Acid Hybridization, Oxidation-Reduction, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Terpenes/*metabolism
  • topic2: Metabolism

Reference

@idauthorscataloguedoi/urltitle
4616Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 12140)https://www.dsmz.de/collection/catalogue/details/culture/DSM-12140
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
55111Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 41528)https://www.ccug.se/strain?id=41528
68369Automatically annotated from API 20NE
68382Automatically annotated from API zym
83090Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID49241.1StrainInfo: A central database for resolving microbial strain identifiers