Strain identifier

BacDive ID: 13914

Type strain: Yes

Species: Thauera terpenica

Strain Designation: 58Eu

Strain history: CIP <- 1999, CCUG <- 1998, J. Harder, Max Planck Inst., Bremen, Germany: strain 58Eu

NCBI tax ID(s): 76113 (species)

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General

@ref: 4615

BacDive-ID: 13914

DSM-Number: 12139

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Thauera terpenica 58Eu is an aerobe, mesophilic, Gram-negative bacterium that was isolated from ditch in a forest.

NCBI tax id

  • NCBI tax id: 76113
  • Matching level: species

strain history

@refhistory
4615<- J. Harder; 58Eu
123448CIP <- 1999, CCUG <- 1998, J. Harder, Max Planck Inst., Bremen, Germany: strain 58Eu

doi: 10.13145/bacdive13914.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Rhodocyclales
  • family: Zoogloeaceae
  • genus: Thauera
  • species: Thauera terpenica
  • full scientific name: Thauera terpenica Foss and Harder 1999

@ref: 4615

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Rhizobiales

family: Zoogloeaceae

genus: Thauera

species: Thauera terpenica

full scientific name: Thauera terpenica Foss and Harder 1999

strain designation: 58Eu

type strain: yes

Morphology

cell morphology

  • @ref: 123448
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 4615
  • incubation period: >14 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
4615R2A MEDIUM (DSMZ Medium 830)yeshttps://mediadive.dsmz.de/medium/830Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
4615ANAEROBIC THAUERA MEDIUM (DSMZ Medium 1315)yeshttps://mediadive.dsmz.de/medium/1315Name: ANAEROBIC THAUERA MEDIUM (DSMZ Medium 1315) Composition: NaHCO3 2.52 g/l Na2HPO4 x 2 H2O 1.45 g/l K-acetate 0.98 g/l KNO3 0.85 g/l MgSO4 x 7 H2O 0.5 g/l NH4Cl 0.3 g/l KH2PO4 0.25 g/l NaCl 0.05 g/l MnSO4 0.01 g/l CaCl2 0.01 g/l HCl 0.0025 g/l FeCl2 x 4 H2O 0.0015 g/l NaOH 0.001 g/l alpha-lipoic acid 0.00025 g/l Riboflavin 0.00025 g/l Thiamine-HCl x 2 H2O 0.00025 g/l Vitamin B12 0.00025 g/l Pantothenic acid 0.00025 g/l p-Aminobenzoic acid 0.00025 g/l CoCl2 x 6 H2O 0.00019 g/l Nicotinic acid 0.000125 g/l Nicotine amide 0.000125 g/l Folic acid 0.0001 g/l MnCl2 x 4 H2O 0.0001 g/l Biotin 0.0001 g/l ZnCl2 7e-05 g/l Pyridoxamine hydrochloride 5e-05 g/l Na2MoO4 x 2 H2O 3.6e-05 g/l NiCl2 x 6 H2O 2.4e-05 g/l Na2WO4 x 2 H2O 8e-06 g/l Na2SeO3 x 5 H2O 6e-06 g/l H3BO3 6e-06 g/l CuCl2 x 2 H2O 2e-06 g/l Distilled water
39416MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
123448CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
4615positivegrowth30mesophilic
39416positivegrowth30mesophilic
55110positivegrowth30mesophilic
123448positivegrowth15-41
123448nogrowth5psychrophilic
123448nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
4615aerobe
4615anaerobe
55110aerobe

halophily

@refsaltgrowthtested relationconcentration
123448NaClpositivegrowth0-2 %
123448NaClnogrowth4 %
123448NaClnogrowth6 %
123448NaClnogrowth8 %
123448NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1234484853esculin-hydrolysis
123448606565hippurate+hydrolysis
12344817632nitrate+builds gas from
12344817632nitrate+reduction
12344816301nitrite+builds gas from
12344816301nitrite+reduction
12344815792malonate-assimilation
12344817234glucose-degradation

antibiotic resistance

  • @ref: 123448
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 123448
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12344815688acetoin-
12344817234glucose-

enzymes

@refvalueactivityec
123448oxidase-
123448beta-galactosidase-3.2.1.23
123448alcohol dehydrogenase-1.1.1.1
123448gelatinase-
123448amylase-
123448DNase-
123448caseinase-3.4.21.50
123448catalase+1.11.1.6
123448tween esterase-
123448gamma-glutamyltransferase+2.3.2.2
123448lecithinase-
123448lipase-
123448lysine decarboxylase-4.1.1.18
123448ornithine decarboxylase-4.1.1.17
123448phenylalanine ammonia-lyase-4.3.1.24
123448protease-
123448tryptophan deaminase-
123448urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase-3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123448-+++-+-----+--------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
4615ditch in a forestGermanyDEUEurope
55110Ditch in a forestGermanyDEUEurope
123448Environment, Ditch in a forestGermanyDEUEurope

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Forest

taxonmaps

  • @ref: 69479
  • File name: preview.99_6080.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_129;96_594;97_3564;98_4511;99_6080&stattab=map
  • Last taxonomy: Thauera terpenica subclade
  • 16S sequence: AJ005817
  • Sequence Identity:
  • Total samples: 3052
  • soil counts: 51
  • aquatic counts: 2844
  • animal counts: 129
  • plant counts: 28

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
46151Risk group (German classification)
1234481Risk group (French classification)

Sequence information

16S sequences

  • @ref: 20218
  • description: Thauera terpenica 16S rRNA gene, type strain 58Eu
  • accession: AJ005817
  • length: 1484
  • database: ena
  • NCBI tax ID: 1348657

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Thauera terpenica 58EuGCA_000443165contigncbi1348657
66792Thauera terpenica 58Eu1348657.5wgspatric1348657
66792Thauera terpenica 58Eu2579778713draftimg1348657

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes90.955no
gram-positiveno98.247no
anaerobicno93.289no
halophileno77.574no
spore-formingno96.284no
thermophileno97.261yes
glucose-utilno85.743no
aerobicyes57.621no
flagellatedyes76.307no
glucose-fermentno91.773no

External links

@ref: 4615

culture collection no.: CCUG 41527, DSM 12139, CIP 105982

straininfo link

  • @ref: 83089
  • straininfo: 49240

literature

  • topic: Phylogeny
  • Pubmed-ID: 9841126
  • title: Thauera linaloolentis sp. nov. and Thauera terpenica sp. nov., isolated on oxygen-containing monoterpenes (linalool, menthol, and eucalyptol) nitrate.
  • authors: Foss S, Harder J
  • journal: Syst Appl Microbiol
  • DOI: 10.1016/s0723-2020(98)80046-5
  • year: 1998
  • mesh: Acyclic Monoterpenes, Anaerobiosis, Base Composition, Biodegradation, Environmental, Culture Media, *Cyclohexanols, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Eucalyptol, Fatty Acids/analysis, Gram-Negative Facultatively Anaerobic Rods/*classification/growth & development/isolation & purification/*metabolism, Menthol/analogs & derivatives/metabolism, *Monoterpenes, Nitrates/*metabolism, Nucleic Acid Hybridization, Oxidation-Reduction, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Terpenes/*metabolism
  • topic2: Metabolism

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
4615Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 12139)https://www.dsmz.de/collection/catalogue/details/culture/DSM-12139
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
39416Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/18061
55110Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 41527)https://www.ccug.se/strain?id=41527
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
83089Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID49240.1StrainInfo: A central database for resolving microbial strain identifiers
123448Curators of the CIPCollection of Institut Pasteur (CIP 105982)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105982