Strain identifier

BacDive ID: 13913

Type strain: Yes

Species: Thauera linaloolentis

Strain Designation: 47Lol

Strain history: CIP <- 1999, CCUG <- 1998, J. Harder, Max,Planck Inst., Bremen, Germany: strain 47Lol

NCBI tax ID(s): 1123367 (strain), 76112 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 4614

BacDive-ID: 13913

DSM-Number: 12138

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, motile

description: Thauera linaloolentis 47Lol is an aerobe, mesophilic, motile bacterium that was isolated from activated sludge.

NCBI tax id

NCBI tax idMatching level
76112species
1123367strain

strain history

@refhistory
4614<- J. Harder; 47Lol
67770IAM 15112 <-- DSM 12138 <-- J. Harder 47Lol.
120609CIP <- 1999, CCUG <- 1998, J. Harder, Max,Planck Inst., Bremen, Germany: strain 47Lol

doi: 10.13145/bacdive13913.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Rhodocyclales
  • family: Zoogloeaceae
  • genus: Thauera
  • species: Thauera linaloolentis
  • full scientific name: Thauera linaloolentis Foss and Harder 1999

@ref: 4614

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Rhizobiales

family: Zoogloeaceae

genus: Thauera

species: Thauera linaloolentis

full scientific name: Thauera linaloolentis Foss and Harder 1999

strain designation: 47Lol

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480yes99.559
69480100negative
120609nonegativerod-shaped

colony morphology

  • @ref: 120609

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
4614NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
39415MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
120609CIP Medium 29yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=29
120609CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
4614positivegrowth30mesophilic
39415positivegrowth30mesophilic
55109positivegrowth30mesophilic
67770positivegrowth30mesophilic
120609positivegrowth25-41
120609nogrowth5psychrophilic
120609nogrowth15psychrophilic
120609nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
55109aerobe
120609obligate aerobe

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.977

halophily

@refsaltgrowthtested relationconcentration
120609NaClpositivegrowth0-4 %
120609NaClnogrowth6 %
120609NaClnogrowth8 %
120609NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1206094853esculin-hydrolysis
120609606565hippurate+hydrolysis
12060917632nitrate+builds gas from
12060917632nitrate+reduction
12060916301nitrite+builds gas from
12060916301nitrite+reduction
12060915792malonate-assimilation
12060917234glucose-degradation
12060917632nitrate+respiration

antibiotic resistance

  • @ref: 120609
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 120609
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12060915688acetoin-
12060917234glucose-

enzymes

@refvalueactivityec
120609oxidase+
120609beta-galactosidase-3.2.1.23
120609alcohol dehydrogenase-1.1.1.1
120609gelatinase-
120609amylase-
120609DNase-
120609caseinase-3.4.21.50
120609catalase+1.11.1.6
120609tween esterase-
120609gamma-glutamyltransferase-2.3.2.2
120609lecithinase-
120609lipase-
120609lysine decarboxylase-4.1.1.18
120609ornithine decarboxylase-4.1.1.17
120609phenylalanine ammonia-lyase+4.3.1.24
120609protease-
120609tryptophan deaminase-
120609urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120609-++--+----++--------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
120609-------------------------------------------------+-----------+-+-----------+---++---+---++++++--+--

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
4614activated sludgeGermanyDEUEurope
55109Activated sludgeGermanyDEUEurope
67770Activated sewage sludge
120609Environment, Activated sludgeGermanyDEUEurope

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Waste
  • Cat3: #Activated sludge

taxonmaps

  • @ref: 69479
  • File name: preview.99_3282.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_129;96_594;97_2050;98_2517;99_3282&stattab=map
  • Last taxonomy: Thauera linaloolentis
  • 16S sequence: AJ005816
  • Sequence Identity:
  • Total samples: 10
  • aquatic counts: 7
  • animal counts: 2
  • plant counts: 1

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
46141Risk group (German classification)
1206091Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Thauera linaloolentis 16S rRNA gene, type strain 47LolAJ0058161482ena1123367
20218Thauera linaloolentis gene for 16S rRNA, partial sequence, strain: NBRC 102519AB6818531463ena1123367

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Thauera linaloolentis 47Lol = DSM 121381123367.4wgspatric1123367
66792Thauera linaloolentis 47Lol = DSM 121381123367.11wgspatric1123367
66792Thauera linaloolentis DSM 121382556921623draftimg1123367
66792Thauera linaloolentis 47Lol, DSM 121382531839277draftimg1123367
67770Thauera linaloolentis 47Lol = DSM 12138GCA_000310205contigncbi1123367
67770Thauera linaloolentis 47Lol = DSM 12138GCA_000621305scaffoldncbi1123367

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno87no
motileyes91.894no
flagellatedyes89.745no
gram-positiveno98.709no
anaerobicno93.521no
aerobicyes80.947yes
halophileno92.971no
spore-formingno94.781no
thermophileno98.833yes
glucose-utilno89.91no
glucose-fermentno88.359no

External links

@ref: 4614

culture collection no.: DSM 12138, CCUG 41526, JCM 21573, CIP 105981, IAM 15112

straininfo link

  • @ref: 83088
  • straininfo: 49239

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny9841126Thauera linaloolentis sp. nov. and Thauera terpenica sp. nov., isolated on oxygen-containing monoterpenes (linalool, menthol, and eucalyptol) nitrate.Foss S, Harder JSyst Appl Microbiol10.1016/s0723-2020(98)80046-51998Acyclic Monoterpenes, Anaerobiosis, Base Composition, Biodegradation, Environmental, Culture Media, *Cyclohexanols, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Eucalyptol, Fatty Acids/analysis, Gram-Negative Facultatively Anaerobic Rods/*classification/growth & development/isolation & purification/*metabolism, Menthol/analogs & derivatives/metabolism, *Monoterpenes, Nitrates/*metabolism, Nucleic Acid Hybridization, Oxidation-Reduction, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Terpenes/*metabolismMetabolism
Enzymology26979141Linalool isomerase, a membrane-anchored enzyme in the anaerobic monoterpene degradation in Thauera linaloolentis 47Lol.Marmulla R, Safaric B, Markert S, Schweder T, Harder JBMC Biochem10.1186/s12858-016-0062-02016Acyclic Monoterpenes, Cell Wall/metabolism, Centrifugation, Density Gradient, Chromatography, Liquid, Electrophoresis, Polyacrylamide Gel, Hydro-Lyases/metabolism, Hydrogen-Ion Concentration, Isomerases/chemistry/genetics/*metabolism, Isomerism, Kinetics, Monoterpenes/chemistry/*metabolism, Recombinant Proteins/biosynthesis/chemistry/isolation & purification, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Spheroplasts/isolation & purification/metabolism, Substrate Specificity, Temperature, Terpenes/chemistry/metabolism, Thauera/chemistry/*enzymologyMetabolism
Metabolism27118314The anaerobic linalool metabolism in Thauera linaloolentis 47 Lol.Marmulla R, Cala EP, Markert S, Schweder T, Harder JBMC Microbiol10.1186/s12866-016-0693-82016Acyclic Monoterpenes, Anaerobiosis, Bacterial Proteins/*genetics/metabolism, DNA Transposable Elements, Genome, Bacterial, Monoterpenes/*metabolism, Mutagenesis, Insertional, Thauera/genetics/*growth & developmentGenetics
Phylogeny30113299Thauera hydrothermalis sp. nov., a thermophilic bacterium isolated from hot spring.Yang L, Muhadesi JB, Wang MM, Wang BJ, Liu SJ, Jiang CYInt J Syst Evol Microbiol10.1099/ijsem.0.0029602018Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Hot Springs/*microbiology, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Thauera/*classification/genetics/isolation & purification, Tibet, Ubiquinone/chemistryTranscriptome
Enzymology33296469Differential expression of clade I and II N2O reductase genes in denitrifying Thauera linaloolentis 47LolT under different nitrogen conditions.Semedo M, Wittorf L, Hallin S, Song BFEMS Microbiol Lett10.1093/femsle/fnaa2052020Gene Expression Profiling, Gene Expression Regulation, Bacterial/drug effects, Nitrates/pharmacology, Nitrogen/*pharmacology, Nitrous Oxide/pharmacology, Oxidoreductases/genetics, Soil Microbiology, Thauera/*enzymology/*geneticsPathogenicity
Phylogeny35596013Thauera sedimentorum sp. nov., Isolated from Coastal Sediment.Baek J, Jaikwang N, Kim JH, Yoon JH, Lee JS, Sukhoom A, Kim WCurr Microbiol10.1007/s00284-022-02890-w2022Bacterial Typing Techniques, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nucleic Acid Hybridization, *Phospholipids/chemistry, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Thauera/geneticsTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
4614Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 12138)https://www.dsmz.de/collection/catalogue/details/culture/DSM-12138
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
39415Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/18060
55109Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 41526)https://www.ccug.se/strain?id=41526
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
83088Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID49239.1StrainInfo: A central database for resolving microbial strain identifiers
120609Curators of the CIPCollection of Institut Pasteur (CIP 105981)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105981