Strain identifier

BacDive ID: 13908

Type strain: Yes

Species: Thauera aromatica

Strain Designation: K172

Strain history: <- G. Fuchs <- A. Tschech; K172 (Pseudomonas sp.)

NCBI tax ID(s): 59405 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 2936

BacDive-ID: 13908

DSM-Number: 6984

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, motile

description: Thauera aromatica K172 is an aerobe, mesophilic, motile bacterium that was isolated from sewage sludge.

NCBI tax id

  • NCBI tax id: 59405
  • Matching level: species

strain history

  • @ref: 2936
  • history: <- G. Fuchs <- A. Tschech; K172 (Pseudomonas sp.)

doi: 10.13145/bacdive13908.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Rhodocyclales
  • family: Zoogloeaceae
  • genus: Thauera
  • species: Thauera aromatica
  • full scientific name: Thauera aromatica Anders et al. 1995

@ref: 2936

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Rhizobiales

family: Zoogloeaceae

genus: Thauera

species: Thauera aromatica

full scientific name: Thauera aromatica Anders et al. 1995

strain designation: K172

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram stain
69480yes99.167
6948099.999negative

multimedia

  • @ref: 2936
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_6984.jpg
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
2936R2A MEDIUM (DSMZ Medium 830)yeshttps://mediadive.dsmz.de/medium/830Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
2936THAUERA AROMATICA MEDIUM (DSMZ Medium 586)yeshttps://mediadive.dsmz.de/medium/586Name: THAUERA AROMATICA MEDIUM (DSMZ Medium 586) Composition: K2HPO4 5.83251 g/l KNO3 1.97044 g/l KH2PO4 0.803941 g/l Na-benzoate 0.70936 g/l NH4Cl 0.522168 g/l MgSO4 x 7 H2O 0.197044 g/l CaCl2 x 2 H2O 0.0246305 g/l HCl 0.0246305 g/l FeCl2 x 4 H2O 0.0147783 g/l CoCl2 x 6 H2O 0.00187192 g/l MnCl2 x 4 H2O 0.000985222 g/l ZnCl2 0.000689655 g/l Na2MoO4 x 2 H2O 0.00035468 g/l p-Aminobenzoic acid 0.000246305 g/l alpha-lipoic acid 0.000246305 g/l Riboflavin 0.000246305 g/l Pantothenic acid 0.000246305 g/l Vitamin B12 0.000246305 g/l Thiamine-HCl x 2 H2O 0.000246305 g/l NiCl2 x 6 H2O 0.000236453 g/l Nicotine amide 0.000123153 g/l Nicotinic acid 0.000123153 g/l Folic acid 9.85222e-05 g/l Biotin 9.85222e-05 g/l H3BO3 5.91133e-05 g/l Pyridoxamine hydrochloride 4.92611e-05 g/l CuCl2 x 2 H2O 1.97044e-05 g/l Distilled water

culture temp

  • @ref: 2936
  • growth: positive
  • type: growth
  • temperature: 30
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
2936aerobe
2936anaerobe

spore formation

@refspore formationconfidence
69481no99
69480no99.994

Isolation, sampling and environmental information

isolation

  • @ref: 2936
  • sample type: sewage sludge
  • geographic location: Konstanz
  • country: Germany
  • origin.country: DEU
  • continent: Europe

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Waste
  • Cat3: #Sewage sludge

Safety information

risk assessment

  • @ref: 2936
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 20218
  • description: T.aromatica 16S rRNA gene
  • accession: X77118
  • length: 1500
  • database: ena
  • NCBI tax ID: 59405

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Thauera aromatica K172GCA_003030465completencbi44139
66792Thauera aromatica K17244139.8completepatric44139
66792Thauera aromatica K1722791355019completeimg44139

GC content

  • @ref: 2936
  • GC-content: 67.5
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno99no
flagellatedyes67.786no
gram-positiveno99.078no
anaerobicno90.13no
halophileno93.131no
spore-formingno94.314no
glucose-utilno81.404no
aerobicno72.001no
thermophileno96.138yes
motileyes89.403no
glucose-fermentno90.933no

External links

@ref: 2936

culture collection no.: DSM 6984, CIP 107765

straininfo link

  • @ref: 83083
  • straininfo: 48343

literature

  • topic: Phylogeny
  • Pubmed-ID: 29236233
  • title: Thauera sinica sp. nov., a phenol derivative-degrading bacterium isolated from activated sludge.
  • authors: Qiao N, Xi L, Zhang J, Liu D, Ge B, Liu J
  • journal: Antonie Van Leeuwenhoek
  • DOI: 10.1007/s10482-017-0993-5
  • year: 2017
  • mesh: Bacterial Typing Techniques, Base Composition/genetics, DNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sewage/microbiology, Thauera/*genetics/isolation & purification
  • topic2: Enzymology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
2936Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 6984)https://www.dsmz.de/collection/catalogue/details/culture/DSM-6984
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
83083Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID48343.1StrainInfo: A central database for resolving microbial strain identifiers