Strain identifier

BacDive ID: 13896

Type strain: Yes

Species: Dechloromonas hortensis

Strain Designation: MA-1

Strain history: <- A. Wolterink, Wageningen Univ.; MA-1

NCBI tax ID(s): 337779 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 6009

BacDive-ID: 13896

DSM-Number: 15637

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, motile

description: Dechloromonas hortensis MA-1 is an anaerobe, mesophilic, motile bacterium that was isolated from garden soil.

NCBI tax id

  • NCBI tax id: 337779
  • Matching level: species

strain history

  • @ref: 6009
  • history: <- A. Wolterink, Wageningen Univ.; MA-1

doi: 10.13145/bacdive13896.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Rhodocyclales
  • family: Azonexaceae
  • genus: Dechloromonas
  • species: Dechloromonas hortensis
  • full scientific name: Dechloromonas hortensis Wolterink et al. 2005

@ref: 6009

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Rhizobiales

family: Azonexaceae

genus: Dechloromonas

species: Dechloromonas hortensis

full scientific name: Dechloromonas hortensis Wolterink et al. 2005

strain designation: MA-1

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram stain
69480yes99.1
69480100negative

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6009DECHLOROMONAS MEDIUM (DSMZ Medium 944a)yeshttps://mediadive.dsmz.de/medium/944aName: MEDIUM MODIFIED FOR DECHLOROMONAS SPP. (DSMZ Medium 944a) Composition: NaHCO3 3.73 g/l Na-acetate x 3 H2O 2.72 g/l NaClO3 1.06 g/l Na2HPO4 0.53 g/l NH4HCO3 0.44 g/l KH2PO4 0.41 g/l Na2SO4 0.2 g/l CaCl2 0.11 g/l MgCl2 0.1 g/l Yeast extract 0.0999999 g/l HCl 0.0025 g/l NaOH 0.002 g/l FeCl2 x 4 H2O 0.0015 g/l Resazurin 0.0005 g/l CoCl2 x 6 H2O 0.00019 g/l MnCl2 x 4 H2O 0.0001 g/l ZnCl2 7e-05 g/l p-Aminobenzoic acid 5e-05 g/l alpha-lipoic acid 5e-05 g/l Pantothenic acid 5e-05 g/l Thiamine-HCl x 2 H2O 5e-05 g/l Riboflavin 5e-05 g/l Vitamin B12 5e-05 g/l Na2MoO4 x 2 H2O 3.6e-05 g/l Nicotine amide 2.5e-05 g/l Nicotinic acid 2.5e-05 g/l NiCl2 x 6 H2O 2.4e-05 g/l Biotin 2e-05 g/l Folic acid 2e-05 g/l Na2WO4 x 2 H2O 1.6e-05 g/l Na2SeO3 x 5 H2O 1.2e-05 g/l Pyridoxamine hydrochloride 1e-05 g/l H3BO3 6e-06 g/l CuCl2 x 2 H2O 2e-06 g/l Distilled water
6009MEDIUM R2A MODIFIED (DSMZ Medium 830a)yeshttps://mediadive.dsmz.de/medium/830aName: MEDIUM R2A MODIFIED (DSMZ Medium 830a) Composition: Agar 15.0 g/l Yeast extract 0.5 g/l Proteose peptone 0.5 g/l Casamino acids 0.5 g/l Glucose 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Tween 80 Distilled water

culture temp

  • @ref: 6009
  • growth: positive
  • type: growth
  • temperature: 30
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
6009anaerobe
6009microaerophile

spore formation

@refspore formationconfidence
69481no100
69480no99.97

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate-assimilation
6836917128adipate-assimilation
6836927689decanoate-assimilation
6836924265gluconate-assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose-assimilation
683695291gelatin-hydrolysis
683694853esculin+hydrolysis
6836916199urea+hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate+reduction

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68369cytochrome oxidase+1.9.3.1
68369gelatinase-
68369beta-glucosidase+3.2.1.21
68369urease+3.5.1.5
68369arginine dihydrolase-3.5.3.6

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
6009+---++--------------+

Isolation, sampling and environmental information

isolation

  • @ref: 6009
  • sample type: garden soil
  • country: Netherlands
  • origin.country: NLD
  • continent: Europe

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Soil
#Engineered#Agriculture#Garden

Safety information

risk assessment

  • @ref: 6009
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 6009
  • description: Dechloromonas hortensis strain MA-1 16S ribosomal RNA gene, partial sequence
  • accession: AY277621
  • length: 1511
  • database: ena
  • NCBI tax ID: 337779

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Dechloromonas hortensis MA-1GCA_009469765contigncbi337779
66792Dechloromonas hortensis strain MA-1337779.3wgspatric337779

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
gram-positiveno99.169no
anaerobicno87.651no
halophileno96.016no
spore-formingno94.649no
glucose-utilno67.87no
motileyes91.41no
flagellatedyes79.818no
thermophileno98.471yes
aerobicno63.81no
glucose-fermentno90.174yes

External links

@ref: 6009

culture collection no.: DSM 15637, ATCC BAA 776

straininfo link

  • @ref: 83071
  • straininfo: 265595

literature

  • topic: Phylogeny
  • Pubmed-ID: 16166710
  • title: Dechloromonas hortensis sp. nov. and strain ASK-1, two novel (per)chlorate-reducing bacteria, and taxonomic description of strain GR-1.
  • authors: Wolterink A, Kim S, Muusse M, Kim IS, Roholl PJM, van Ginkel CG, Stams AJM, Kengen SWM
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.63404-0
  • year: 2005
  • mesh: Chlorates/*metabolism, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Genes, rRNA, Molecular Sequence Data, Nucleic Acid Hybridization, Oxidation-Reduction, Perchlorates/*metabolism, RNA, Ribosomal, 16S/genetics, Rhodocyclaceae/*classification/genetics/isolation & purification/metabolism, Sequence Analysis, DNA, Sewage/*microbiology, *Soil Microbiology, Species Specificity, Waste Disposal, Fluid/methods, Water Pollution, Chemical
  • topic2: Metabolism

Reference

@idauthorscataloguedoi/urltitle
6009Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15637)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15637
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68369Automatically annotated from API 20NE
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
83071Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID265595.1StrainInfo: A central database for resolving microbial strain identifiers