Strain identifier

BacDive ID: 138959

Type strain: No

Species: Bacillus subtilis

Strain Designation: CB 22

Strain history: CIP <- 1976, G. Balassa: strain CB 22

NCBI tax ID(s): 1423 (species)

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General

@ref: 40959

BacDive-ID: 138959

keywords: Bacteria, obligate aerobe, spore-forming, mesophilic, Gram-positive, motile, rod-shaped

description: Bacillus subtilis CB 22 is an obligate aerobe, spore-forming, mesophilic bacterium of the family Bacillaceae.

NCBI tax id

  • NCBI tax id: 1423
  • Matching level: species

strain history

@refhistory
409591976, G. Balassa: strain CB 22
40959CIP <- 1976, G. Balassa: strain CB 22

doi: 10.13145/bacdive138959.20241212.9.2

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Bacillus
  • species: Bacillus subtilis
  • full scientific name: Bacillus subtilis (Ehrenberg 1835) Cohn 1872 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Vibrio subtilis

@ref: 40959

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Caryophanales

family: Bacillaceae

genus: Bacillus

species: Bacillus subtilis

strain designation: CB 22

type strain: no

Morphology

cell morphology

  • @ref: 40959
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 40959
  • hemolysis ability: 1

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
40959MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
40959CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3
40959CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72
40959CIP Medium 29yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=29

culture temp

@refgrowthtypetemperature
40959positivegrowth30
40959positivegrowth22-45
40959nogrowth10
40959nogrowth55

culture pH

  • @ref: 40959
  • ability: positive
  • type: growth
  • pH: 6

Physiology and metabolism

oxygen tolerance

  • @ref: 40959
  • oxygen tolerance: obligate aerobe

spore formation

  • @ref: 40959
  • spore formation: yes

halophily

  • @ref: 40959
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-10 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371xylitol-builds acid from17151
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose-builds acid from17306
68371cellobiose+builds acid from17057
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371arbutin+builds acid from18305
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
40959esculin+hydrolysis4853
40959hippurate-hydrolysis606565
40959nitrate+reduction17632
40959nitrite-reduction16301
40959nitrate-respiration17632

metabolite production

  • @ref: 40959
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
4095915688acetoin+
4095917234glucose-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase+3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
40959oxidase+
40959beta-galactosidase+3.2.1.23
40959alcohol dehydrogenase-1.1.1.1
40959gelatinase+
40959amylase+
40959DNase+
40959caseinase+3.4.21.50
40959catalase+1.11.1.6
40959tween esterase+
40959gamma-glutamyltransferase+2.3.2.2
40959lecithinase-
40959lipase-
40959lysine decarboxylase-4.1.1.18
40959ornithine decarboxylase+4.1.1.17
40959protease+
40959urease+3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
40959-+++-+----++++-++---

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
40959---+/-+/------+++---+/-+/-+/----+/-++++---++---+/-+/--+/-+/-------+/---

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
40959++-++--+-++----++++++-+--------++++-+----+--+----+-----+---++-------------------+-----+--+++-+-----

Safety information

risk assessment

  • @ref: 40959
  • biosafety level: 1
  • biosafety level comment: Risk group (French classification)

External links

@ref: 40959

culture collection no.: CIP 76.26

straininfo link

  • @ref: 95579
  • straininfo: 67469

literature

  • topic: Cultivation
  • Pubmed-ID: 15574932
  • title: Pressure inactivation of Bacillus endospores.
  • authors: Margosch D, Ganzle MG, Ehrmann MA, Vogel RF
  • journal: Appl Environ Microbiol
  • DOI: 10.1128/AEM.70.12.7321-7328.2004
  • year: 2004
  • mesh: Bacillus/classification/*growth & development/*physiology, Bacillus subtilis/genetics/growth & development/physiology, Colony Count, Microbial, Culture Media, Daucus carota/microbiology, Food Preservation/methods, *Hot Temperature, *Hydrostatic Pressure, Picolinic Acids/metabolism, Spores, Bacterial/physiology, Temperature
  • topic2: Stress

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
40959Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2076.26Collection of Institut Pasteur (CIP 76.26)
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
95579Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID67469.1