Strain identifier

BacDive ID: 13893

Type strain: Yes

Species: Azospira restricta

Strain history: <- W. M. Moe; SUA2 <- H.-S. Bae

NCBI tax ID(s): 404405 (species)

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General

@ref: 7626

BacDive-ID: 13893

DSM-Number: 18626

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Azospira restricta DSM 18626 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from groundwater.

NCBI tax id

  • NCBI tax id: 404405
  • Matching level: species

strain history

  • @ref: 7626
  • history: <- W. M. Moe; SUA2 <- H.-S. Bae

doi: 10.13145/bacdive13893.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Rhodocyclales
  • family: Rhodocyclaceae
  • genus: Azospira
  • species: Azospira restricta
  • full scientific name: Azospira restricta Bae et al. 2007

@ref: 7626

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Rhizobiales

family: Rhodocyclaceae

genus: Azospira

species: Azospira restricta

full scientific name: Azospira restricta Bae et al. 2007

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
32100negative1.85 µm0.5 µmrod-shapedyes
69480yes98.946
69480negative99.998

colony morphology

  • @ref: 60664
  • incubation period: 2-3 days

Culture and growth conditions

culture medium

  • @ref: 7626
  • name: R2A MEDIUM (DSMZ Medium 830)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/830
  • composition: Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
7626positivegrowth37mesophilic
32100positivegrowth20-40
32100positiveoptimum37mesophilic
60664positivegrowth37mesophilic

culture pH

  • @ref: 32100
  • ability: positive
  • type: optimum
  • pH: 7

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
32100aerobe
60664aerobe

spore formation

@refspore formationconfidence
32100no
69481no97
69480no99.991

halophily

@refsaltgrowthtested relationconcentration
32100NaClpositivegrowth0.0-2.0 %
32100NaClpositiveoptimum1 %

metabolite utilization

  • @ref: 32100
  • Chebi-ID: 17148
  • metabolite: putrescine
  • utilization activity: +
  • kind of utilization tested: carbon source

enzymes

@refvalueactivityec
32100catalase+1.11.1.6
32100cytochrome oxidase+1.9.3.1

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
7626groundwaterLouisiana, Baton RougeUSAUSANorth America
60664Groundwater

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic
  • Cat3: #Groundwater

taxonmaps

  • @ref: 69479
  • File name: preview.99_163652.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_129;96_29574;97_41973;98_54795;99_163652&stattab=map
  • Last taxonomy: Azospira restricta subclade
  • 16S sequence: DQ974114
  • Sequence Identity:
  • Total samples: 272
  • soil counts: 62
  • aquatic counts: 181
  • animal counts: 8
  • plant counts: 21

Safety information

risk assessment

  • @ref: 7626
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 7626
  • description: Azospira restricta strain SUA2 16S ribosomal RNA gene, partial sequence
  • accession: DQ974114
  • length: 1482
  • database: ena
  • NCBI tax ID: 404405

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Azospira restricta DSM 18626GCA_016858125completencbi404405
66792Azospira restricta strain DSM 18626404405.4completepatric404405

GC content

  • @ref: 32100
  • GC-content: 67.9

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno97no
flagellatedyes89.578no
gram-positiveno99.321yes
anaerobicno92.403yes
aerobicyes59.701yes
halophileno96.087yes
spore-formingno95.135yes
thermophileno93.057yes
glucose-utilno79.259no
motileyes90.29no
glucose-fermentno92.351no

External links

@ref: 7626

culture collection no.: DSM 18626, CCUG 55598, NRRL B-41660, LMG 23819, Sua SUA2, Sua 2

straininfo link

  • @ref: 83068
  • straininfo: 292492

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny17625187Description of Azospira restricta sp. nov., a nitrogen-fixing bacterium isolated from groundwater.Bae HS, Rash BA, Rainey FA, Nobre MF, Tiago I, da Costa MS, Moe WMInt J Syst Evol Microbiol10.1099/ijs.0.64965-02007Bacterial Typing Techniques, Catalase/analysis, Cytoplasmic Granules, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Flagella, Genes, rRNA/genetics, Hydroxybutyrates/metabolism, Molecular Sequence Data, Nitrogen/metabolism, Nitrogen Fixation, Oxidoreductases/analysis, Phylogeny, Polyesters/metabolism, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Rhodocyclaceae/chemistry/*classification/genetics/*isolation & purification, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, *Soil Microbiology, Spores, Bacterial, *Water MicrobiologyMetabolism
Genetics33958413Genome Sequence of the Type Strain Azospira restricta SUA2 (DSM 18626).Mikes MC, Moe WMMicrobiol Resour Announc10.1128/MRA.00156-212021

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
7626Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 18626)https://www.dsmz.de/collection/catalogue/details/culture/DSM-18626
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
32100Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2834528776041
60664Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 55598)https://www.ccug.se/strain?id=55598
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
83068Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID292492.1StrainInfo: A central database for resolving microbial strain identifiers