Strain identifier

BacDive ID: 138865

Type strain: No

Species: Pseudomonas aeruginosa

Strain Designation: EML534, 4821/1952-222

Strain history: CIP <- 1959, H. Kleinmaier, Hyg. Inst., Bonn, Germany: strain 4821/1952-222

NCBI tax ID(s): 287 (species)

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General

@ref: 40836

BacDive-ID: 138865

keywords: genome sequence, Bacteria, obligate aerobe, mesophilic, motile

description: Pseudomonas aeruginosa EML534 is an obligate aerobe, mesophilic, motile bacterium that was isolated from Unknown source.

NCBI tax id

  • NCBI tax id: 287
  • Matching level: species

strain history

  • @ref: 40836
  • history: CIP <- 1959, H. Kleinmaier, Hyg. Inst., Bonn, Germany: strain 4821/1952-222

doi: 10.13145/bacdive138865.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas aeruginosa
  • full scientific name: Pseudomonas aeruginosa (Schroeter 1872) Migula 1900 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Bacterium aeruginosum

@ref: 40836

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas aeruginosa

strain designation: EML534, 4821/1952-222

type strain: no

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480yes99.046
6948099.99negative
40836yesnegativerod-shaped

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
40836MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
40836CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72
40836CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

  • @ref: 40836
  • growth: positive
  • type: growth
  • temperature: 30
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 40836
  • oxygen tolerance: obligate aerobe

spore formation

@refspore formationconfidence
69481no97
69480no99.932

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4083617632nitrate+reduction
4083616301nitrite+reduction

metabolite production

  • @ref: 40836
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
40836oxidase+
40836catalase+1.11.1.6
40836urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)+
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
40836-++++++-+-++--------

Isolation, sampling and environmental information

isolation

  • @ref: 40836
  • sample type: Unknown source

Safety information

risk assessment

  • @ref: 40836
  • biosafety level: 2
  • biosafety level comment: Risk group (French classification)

Sequence information

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Pseudomonas aeruginosa ATCC 33349GCA_001420185scaffoldncbi287
66792Pseudomonas aeruginosa strain ATCC 33349287.2004wgspatric287
66792Pseudomonas aeruginosa ATCC 333492695420489draftimg287

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno97no
flagellatedyes84.725no
gram-positiveno98.447no
anaerobicno98.516no
aerobicyes93.222no
halophileno92.021no
spore-formingno93.657no
thermophileno99.792no
glucose-utilyes89.281no
motileyes88.117no
glucose-fermentno89.397no

External links

@ref: 40836

culture collection no.: CIP 59.34, ATCC 33349, NCTC 11441

straininfo link

  • @ref: 95501
  • straininfo: 7941

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
40836Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2059.34Collection of Institut Pasteur (CIP 59.34)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
95501Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID7941.1