Strain identifier

BacDive ID: 138822

Type strain: No

Species: Klebsiella pneumoniae

Strain Designation: LRA 003 09 73

Strain history: CIP <- 1993, R. Mercier, bioMérieux, La Balme-les-Grottes, France: strain LRA 003 09 73

NCBI tax ID(s): 573 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 8.1 (current version)

General

@ref: 40768

BacDive-ID: 138822

keywords: Bacteria, facultative anaerobe, mesophilic, Gram-negative, rod-shaped

description: Klebsiella pneumoniae LRA 003 09 73 is a facultative anaerobe, mesophilic, Gram-negative bacterium of the family Enterobacteriaceae.

NCBI tax id

  • NCBI tax id: 573
  • Matching level: species

strain history

  • @ref: 40768
  • history: CIP <- 1993, R. Mercier, bioMérieux, La Balme-les-Grottes, France: strain LRA 003 09 73

doi: 10.13145/bacdive138822.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Enterobacteriaceae
  • genus: Klebsiella
  • species: Klebsiella pneumoniae
  • full scientific name: Klebsiella pneumoniae (Schroeter 1886) Trevisan 1887 (Approved Lists 1980)
  • synonyms

    • @ref: 20215
    • synonym: Hyalococcus pneumoniae

@ref: 40768

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacterales

family: Enterobacteriaceae

genus: Klebsiella

species: Klebsiella pneumoniae

strain designation: LRA 003 09 73

type strain: no

Morphology

cell morphology

  • @ref: 40768
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
40768MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
40768CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
40768positivegrowth30mesophilic
40768positivegrowth10-41
40768nogrowth5psychrophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 40768
  • oxygen tolerance: facultative anaerobe

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate+builds acid from
68371gluconate+builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol+builds acid from18333
68371L-fucose+builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose+builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose+builds acid from32528
68371gentiobiose+builds acid from28066
68371xylitol-builds acid from17151
68371glycogen+builds acid from28087
68371starch+builds acid from28017
68371raffinose+builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose+builds acid from28053
68371lactose+builds acid from17716
68371maltose+builds acid from17306
68371cellobiose+builds acid from17057
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371arbutin+builds acid from18305
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside+builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol+builds acid from17924
68371D-mannitol+builds acid from16899
68371myo-inositol+builds acid from17268
68371galactitol+builds acid from16813
68371L-rhamnose+builds acid from62345
68371L-sorbose+builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol+builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose+builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose+builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol+builds acid from17754
40768mannitol+fermentation29864
40768citrate+carbon source16947
40768glucose+fermentation17234
40768lactose+fermentation17716
40768nitrate+reduction17632
40768nitrite-reduction16301
40768malonate+assimilation15792
40768sodium thiosulfate-builds gas from132112
40768glucose+degradation17234

antibiotic resistance

  • @ref: 40768
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 40768
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
4076815688acetoin+
4076817234glucose-

enzymes

@refvalueactivityec
40768oxidase-
40768beta-galactosidase+3.2.1.23
40768alcohol dehydrogenase-1.1.1.1
40768gelatinase-
40768catalase+1.11.1.6
40768lysine decarboxylase+4.1.1.18
40768ornithine decarboxylase-4.1.1.17
40768tryptophan deaminase-
40768urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
40768-+++-++---++++-++---

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
40768+--+++-+-++++++++++-+++/-+++++++++--+++-++-+-++-++-

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
40768+++++++++++++++++++++++++-+--+++++++++---+-+++-+++++-++++--+++++++-+-+--++-+---++++++-+-++++++++--+

Safety information

risk assessment

  • @ref: 40768
  • biosafety level: 2
  • biosafety level comment: Risk group (French classification)

External links

@ref: 40768

culture collection no.: CIP 103687, ATCC 23357

literature

topicPubmed-IDtitleauthorsjournalyearmeshtopic2DOI
Pathogenicity19967999Chemical composition and antibacterial activity of the essential oil of Monticalia andicola (Asteraceae) collected in Venezuela.Baldovino S, Rojas J, Rojas LB, Lucena M, Buitrago A, Morales ANat Prod Commun2009Anti-Bacterial Agents/*chemistry/*pharmacology, Asteraceae/*chemistry, Bacteria/drug effects, Chromatography, Gas, Gas Chromatography-Mass Spectrometry, Microbial Sensitivity Tests, Oils, Volatile/*chemistry/*pharmacology, Venezuela
Enzymology21615035Essential oil composition and antibacteral activity of Vismia baccifera fruits collected from Merida, Venezuela.Rojas J, Buitrago A, Rojas L, Morales A, Lucena M, Baldovino SNat Prod Commun2011Anti-Bacterial Agents/*chemistry, Clusiaceae/*chemistry, Fruit/chemistry, Microbial Sensitivity Tests, Oils, Volatile/*chemistry/isolation & purification, VenezuelaPhylogeny
Pathogenicity21834255Chemical composition and in vitro antibacterial activity of the essential oil of Phthirusa adunca from Venezuelan Andes.Mora FD, Rios N, Rojas LB, Diaz T, Velasco J, Carmona JA, Silva BNat Prod Commun2011Anti-Bacterial Agents/*chemistry/*pharmacology, Bacteria/drug effects, Gas Chromatography-Mass Spectrometry, Loranthaceae/*chemistry, Microbial Sensitivity Tests, Monoterpenes/chemistry/*isolation & purification/pharmacology, Oils, Volatile/*chemistry/*pharmacology, Plant Components, Aerial/chemistry, Plant Oils/*chemistry/pharmacology, VenezuelaEnzymology
Pathogenicity31325570Assessment of antibiotic resistance in bacteriophage-insensitive Klebsiella pneumoniae.Uddin MJ, Kim B, Dawan J, Ding T, Kim JC, Ahn JMicrob Pathog2019Aminoglycosides/genetics, Anti-Bacterial Agents/*pharmacology, Bacteriophages/*physiology, Cefotaxime/pharmacology, Cephalothin/pharmacology, Chloramphenicol/pharmacology, Ciprofloxacin/pharmacology, Drug Resistance, Multiple, Bacterial/*drug effects/genetics, Gene Expression Regulation, Bacterial, Humans, Klebsiella pneumoniae/*drug effects/*virology, Levofloxacin/pharmacology, Phage Therapy, Quinolones/pharmacology, beta-Lactamases/genetics10.1016/j.micpath.2019.103625
35561980Assessment of bacteriophage-encoded endolysin as a potent antimicrobial agent against antibiotic-resistant Salmonella Typhimurium.Kim J, Kim JC, Ahn JMicrob Pathog202210.1016/j.micpath.2022.105576

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
40768Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103687Collection of Institut Pasteur (CIP 103687)
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym