Archive BacDiveID:10.13145/bacdive13881.20230509.8.1

Strain identifier

BacDive ID: 13881

Type strain:

Species: Aromatoleum toluolicum

Strain Designation: T

Strain history: <- J. Zeyer, EAWAG, Swiss Fed. Inst. Aquatic Sci. & Technol., Switzerland; T

NCBI tax ID(s): 29544 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.

General

@ref: 3618

BacDive-ID: 13881

DSM-Number: 9506

keywords: genome sequence, Bacteria, aerobe, mesophilic

description: Aromatoleum toluolicum T is an aerobe, mesophilic bacterium that was isolated from effluent from laboratory aquifer column.

NCBI tax id

NCBI tax id: 29544
Matching level: species

strain history

@ref: 3618
history: <- J. Zeyer, EAWAG, Swiss Fed. Inst. Aquatic Sci. & Technol., Switzerland; T

doi: 10.13145/bacdive13881.20230509.8.1

Name and taxonomic classification

LPSN

@ref: 20215
description: domain/bacteria
keyword: phylum/pseudomonadota
domain: Bacteria
phylum: Pseudomonadota
class: Betaproteobacteria
order: Rhodocyclales
family: Zoogloeaceae
genus: Aromatoleum
species: Aromatoleum toluolicum
full scientific name: Aromatoleum toluolicum Rabus et al. 2019

@ref: 3618

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Rhizobiales

family: Zoogloeaceae

genus: Azoarcus

species: Azoarcus sp.

full scientific name: Azoarcus sp.

strain designation: T

type strain: no

Culture and growth conditions

culture medium

@ref	name	growth	link	composition
3618	R2A MEDIUM (DSMZ Medium 830)	yes	https://mediadive.dsmz.de/medium/830	Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
3618	MINERAL MEDIUM (NAGEL AND ANDREESEN) (DSMZ Medium 461)	yes	https://mediadive.dsmz.de/medium/461	Name: MINERAL MEDIUM (NAGEL AND ANDREESEN) (DSMZ Medium 461) Composition: Na2HPO4 x 2 H2O 1.44135 g/l MgSO4 x 7 H2O 0.497018 g/l NH4Cl 0.298211 g/l KH2PO4 0.248509 g/l NaCl 0.0497018 g/l MnSO4 0.00994036 g/l CaCl2 0.00994036 g/l HCl 0.00248509 g/l FeCl2 x 4 H2O 0.00149105 g/l Thiamine-HCl x 2 H2O 0.000248509 g/l p-Aminobenzoic acid 0.000248509 g/l Vitamin B12 0.000248509 g/l Pantothenic acid 0.000248509 g/l Riboflavin 0.000248509 g/l alpha-lipoic acid 0.000248509 g/l CoCl2 x 6 H2O 0.000188867 g/l Nicotine amide 0.000124254 g/l Nicotinic acid 0.000124254 g/l Folic acid 9.94036e-05 g/l Biotin 9.94036e-05 g/l MnCl2 x 4 H2O 9.94036e-05 g/l ZnCl2 6.95825e-05 g/l Pyridoxamine hydrochloride 4.97018e-05 g/l Na2MoO4 x 2 H2O 3.57853e-05 g/l NiCl2 x 6 H2O 2.38569e-05 g/l H3BO3 5.96421e-06 g/l CuCl2 x 2 H2O 1.98807e-06 g/l Distilled water
3618	NUTRIENT AGAR (DSMZ Medium 1)	yes	https://mediadive.dsmz.de/medium/1	Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water

culture temp

@ref: 3618
growth: positive
type: growth
temperature: 28
range: mesophilic

Physiology and metabolism

oxygen tolerance

@ref	oxygen tolerance
3618	aerobe
3618	anaerobe

compound production

@ref	compound
3618	succinyl-CoA:(R)-benzylsuccinate CoA-transferase
3618	benzylsuccinate synthase

Isolation, sampling and environmental information

isolation

@ref: 3618
sample type: effluent from laboratory aquifer column
country: Switzerland
origin.country: CHE
continent: Europe

isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Laboratory
#Engineered	#Waste	#Wastewater

Sequence information

Genome sequences

@ref: 66792
description: Aromatoleum toluolicum T
accession: GCA_012911005
assembly level: contig
database: ncbi
NCBI tax ID: 90060

Genome-based predictions

predictions

trait	prediction	confidence	training_data
motile	yes	89.512	no
motile	yes	89.512	no
flagellated	yes	80.097	no
flagellated	yes	80.097	no
gram-positive	no	99.097	no
gram-positive	no	99.097	no
anaerobic	no	93.907	no
anaerobic	no	93.907	no
aerobic	no	55.25	no
aerobic	no	55.25	no
halophile	no	89.786	no
halophile	no	89.786	no
spore-forming	no	95.321	no
spore-forming	no	95.321	no
glucose-util	yes	54.084	no
glucose-util	yes	54.084	no
thermophile	no	98.357	yes
thermophile	no	98.357	yes
glucose-ferment	no	89.549	no
glucose-ferment	no	89.549	no

External links

@ref: 3618

culture collection no.: DSM 9506, DSM 19020, LMG 30751

straininfo link

@ref: 83056
straininfo: 48638

literature

topic: Phylogeny
Pubmed-ID: 30762514
title: Aromatoleum gen. nov., a novel genus accommodating the phylogenetic lineage including Azoarcus evansii and related species, and proposal of Aromatoleum aromaticum sp. nov., Aromatoleum petrolei sp. nov., Aromatoleum bremense sp. nov., Aromatoleum toluolicum sp. nov. and Aromatoleum diolicum sp. nov.
authors: Rabus R, Wohlbrand L, Thies D, Meyer M, Reinhold-Hurek B, Kampfer P
journal: Int J Syst Evol Microbiol
DOI: 10.1099/ijsem.0.003244
year: 2019
mesh: Azoarcus, Bacterial Typing Techniques, DNA, Bacterial/genetics, Nucleic Acid Hybridization, *Phylogeny, Proteomics, RNA, Ribosomal, 16S/genetics, Rhodocyclaceae/*classification, Sequence Analysis, DNA
topic2: Transcriptome

Reference

@id	authors	catalogue	doi/url	title
3618	Curators of the DSMZ	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 9506)	https://www.dsmz.de/collection/catalogue/details/culture/DSM-9506
20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.		10.1099/ijsem.0.004332	List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann		https://diaspora-project.de/progress.html#genomes	Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
83056	Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.		10.60712/SI-ID48638.1	StrainInfo: A central database for resolving microbial strain identifiers