Strain identifier

BacDive ID: 138789

Type strain: Yes

Species: Roseomonas gilardii

Strain Designation: 5424

Strain history: CIP <- 1994, ATCC <- D.G. Hollis: strain 5424 <- J.D. Rihs <- G.L. Gilardi

NCBI tax ID(s): 257708 (species)

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General

@ref: 40652

BacDive-ID: 138789

keywords: genome sequence, Bacteria, obligate aerobe, mesophilic, Gram-negative, rod-shaped

description: Roseomonas gilardii 5424 is an obligate aerobe, mesophilic, Gram-negative bacterium that was isolated from Water,drinking quality.

NCBI tax id

  • NCBI tax id: 257708
  • Matching level: species

strain history

  • @ref: 40652
  • history: CIP <- 1994, ATCC <- D.G. Hollis: strain 5424 <- J.D. Rihs <- G.L. Gilardi

doi: 10.13145/bacdive138789.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Rhodospirillales
  • family: Acetobacteraceae
  • genus: Roseomonas
  • species: Roseomonas gilardii
  • full scientific name: Roseomonas gilardii Rihs et al. 1998

@ref: 40652

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhodospirillales

family: Acetobacteraceae

genus: Roseomonas

species: Roseomonas gilardii

strain designation: 5424

type strain: yes

Morphology

cell morphology

  • @ref: 40652
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: no

colony morphology

@refincubation period
513142 days
40652

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
40652MEDIUM 29- Brain heart agaryesDistilled water make up to (1000.000 ml);Brain heart infusion agar (52.000 g)
40652CIP Medium 29yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=29

culture temp

@refgrowthtypetemperaturerange
40652positivegrowth37mesophilic
51314positivegrowth30-37mesophilic
40652positivegrowth37-41
40652nogrowth5psychrophilic
40652nogrowth10psychrophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 40652
  • oxygen tolerance: obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
40652NaClpositivegrowth0-4 %
40652NaClnogrowth6 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate+builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose+builds acid from28847
68371D-tagatose+builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371sucrose+builds acid from17992
68371melibiose+builds acid from28053
68371lactose+builds acid from17716
68371esculin-builds acid from4853
68371amygdalin-builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-glucose-builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
68369malate+assimilation25115
68369adipate-assimilation17128
68369decanoate-assimilation27689
68369gluconate-assimilation24265
68369maltose-assimilation17306
68369N-acetylglucosamine-assimilation59640
68369D-mannitol-assimilation16899
68369D-mannose-assimilation16024
68369L-arabinose-assimilation30849
68369D-glucose-assimilation17634
68369gelatin-hydrolysis5291
68369esculin-hydrolysis4853
68369urea+hydrolysis16199
68369arginine-hydrolysis29016
68369D-glucose-fermentation17634
68369tryptophan-energy source27897
68369nitrate-reduction17632
40652citrate+carbon source16947
40652esculin-hydrolysis4853
40652nitrate-reduction17632
40652nitrite-reduction16301
40652nitrate-respiration17632

antibiotic resistance

  • @ref: 40652
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleno
4065235581indoleno

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68369cytochrome oxidase-1.9.3.1
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease+3.5.1.5
68369arginine dihydrolase-3.5.3.6
40652oxidase-
40652beta-galactosidase-3.2.1.23
40652alcohol dehydrogenase-1.1.1.1
40652gelatinase-
40652catalase+1.11.1.6
40652lysine decarboxylase-4.1.1.18
40652ornithine decarboxylase-4.1.1.17
40652phenylalanine ammonia-lyase-4.3.1.24
40652urease+3.5.1.5

fatty acid profile

  • @reffatty acidpercentageECL
    51314C16:019.616
    51314C18:00.618
    51314C14:0 3OH/C16:1 ISO I1.115.485
    51314C16:0 2OH0.717.233
    51314C16:0 3OH417.52
    51314C16:1 ω7c1.215.819
    51314C18:1 2OH12.219.088
    51314C18:1 ω5c0.617.919
    51314C18:1 ω7c /12t/9t49.117.824
    51314C18:2 ω6,9c/C18:0 ANTE0.517.724
    51314C19:0 CYCLO ω8c10.618.9
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG
  • @reffatty acidpercentageECL
    51314C16:022.416
    51314C16:0 3OH2.517.52
    51314C18:1 2OH419.088
    51314C18:1 ω7c /12t/9t53.817.824
    51314C19:0 CYCLO ω8c17.318.9
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
51314----+------------+---

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
40652-------+/----+/----------+-+/--+/-+/-+/-++++/----------++-----+

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
40652------------------------------------------------++-----------+-++-+--------+---++-------+-------+--

Isolation, sampling and environmental information

isolation

@refsample typesampling dateisolation date
51314Water,drinking quality1980
40652Environment, Potable water1980

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic

Safety information

risk assessment

  • @ref: 40652
  • biosafety level: 1
  • biosafety level comment: Risk group (French classification)

Sequence information

Genome sequences

  • @ref: 66792
  • description: Roseomonas gilardii subsp. gilardii ATCC 49956
  • accession: GCA_023078375
  • assembly level: complete
  • database: ncbi
  • NCBI tax ID: 204527

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedno74.758no
gram-positiveno97.925no
anaerobicno98.768no
aerobicyes94.731no
halophileno91.331no
spore-formingno92.81no
motileyes66.524no
glucose-fermentno91.144yes
thermophileno89.864yes
glucose-utilyes91.608no

External links

@ref: 40652

culture collection no.: CIP 104026, ATCC 49956, CCM 4846, CCUG 33005, ATCC G7933

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny17012549Roseomonas aquatica sp. nov., isolated from drinking water.Gallego V, Sanchez-Porro C, Garcia MT, Ventosa AInt J Syst Evol Microbiol10.1099/ijs.0.64379-02006Bacterial Typing Techniques, Base Composition, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Fresh Water/*microbiology, Genes, rRNA, Methylobacteriaceae/*classification/genetics/*isolation & purification/physiology, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Spain, *Water SupplyGenetics
Phylogeny18523199Roseomonas aerilata sp. nov., isolated from an air sample.Yoo SH, Weon HY, Noh HJ, Hong SB, Lee CM, Kim BY, Kwon SW, Go SJInt J Syst Evol Microbiol10.1099/ijs.0.65385-02008*Air Microbiology, Bacterial Typing Techniques, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Fatty Acids/analysis, Genes, rRNA, Genotype, Korea, Methylobacteriaceae/*classification/genetics/*isolation & purification/physiology, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Species SpecificityGenetics
Phylogeny19406818Transfer of Teichococcus ludipueritiae and Muricoccus roseus to the genus Roseomonas, as Roseomonas ludipueritiae comb. nov. and Roseomonas rosea comb. nov., respectively, and emended description of the genus Roseomonas.Sanchez-Porro C, Gallego V, Busse HJ, Kampfer P, Ventosa AInt J Syst Evol Microbiol10.1099/ijs.0.004820-02009Bacterial Typing Techniques, DNA, Bacterial/analysis/genetics, Fatty Acids/analysis, Genes, rRNA, Methylobacteriaceae/*classification/genetics/physiology, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Species SpecificityPhenotype
Phylogeny23178724Roseomonas aerophila sp. nov., isolated from air.Kim SJ, Weon HY, Ahn JH, Hong SB, Seok SJ, Whang KS, Kwon SWInt J Syst Evol Microbiol10.1099/ijs.0.046482-02012*Air Microbiology, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/analysis, Methylobacteriaceae/*classification/genetics/isolation & purification, Molecular Sequence Data, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, Ubiquinone/analysisGenetics
Phylogeny26552692Roseomonas elaeocarpi sp. nov., isolated from olive (Elaeocarpus hygrophilus Kurz.) phyllosphere.Damtab J, Nutaratat P, Boontham W, Srisuk N, Duangmal K, Yurimoto H, Sakai Y, Muramatsu Y, Nakagawa YInt J Syst Evol Microbiol10.1099/ijsem.0.0007482015Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Elaeocarpaceae/*microbiology, Fatty Acids/chemistry, Methylobacteriaceae/*classification/genetics/isolation & purification, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Pigmentation, Plant Leaves/microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Thailand, Ubiquinone/chemistryGenetics

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
40652Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104026Collection of Institut Pasteur (CIP 104026)
51314Curators of the CCUGhttps://www.ccug.se/strain?id=33005Culture Collection University of Gothenburg (CCUG) (CCUG 33005)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68369Automatically annotated from API 20NE
68371Automatically annotated from API 50CH acid