Strain identifier

BacDive ID: 13855

Type strain: Yes

Species: Celeribacter neptunius

Strain Designation: H 14, H14

Strain history: CIP <- 2008, E. Ivanova, Swinburne Univ., Victoria, Australia: strain H14

NCBI tax ID(s): 588602 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 18234

BacDive-ID: 13855

DSM-Number: 26471

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Celeribacter neptunius H 14 is a facultative anaerobe, mesophilic, Gram-negative bacterium that was isolated from seawater.

NCBI tax id

  • NCBI tax id: 588602
  • Matching level: species

strain history

@refhistory
376942008, E. Ivanova, Swinbure Univ., Victoria, Australia: strain H14
18234<- CIP <- E. Ivanova, Swinburne Univ., Victoria, Australia
121272CIP <- 2008, E. Ivanova, Swinburne Univ., Victoria, Australia: strain H14

doi: 10.13145/bacdive13855.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Rhodobacterales
  • family: Roseobacteraceae
  • genus: Celeribacter
  • species: Celeribacter neptunius
  • full scientific name: Celeribacter neptunius Ivanova et al. 2010

@ref: 18234

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhodobacterales

family: Rhodobacteraceae

genus: Celeribacter

species: Celeribacter neptunius

full scientific name: Celeribacter neptunius Ivanova et al. 2010

strain designation: H 14, H14

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
29493negative0.8-1.8 µm0.4-0.9 µmrod-shapedyes
69480negative99.997
121272negativerod-shapedyes

colony morphology

  • @ref: 18234
  • incubation period: 2-3 days

pigmentation

  • @ref: 29493
  • production: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
18234BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://mediadive.dsmz.de/medium/514Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
37694Marine agar (MA)yesDistilled water make up to (1000.000 ml);Marine agar (55.100 g)
121272CIP Medium 13yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13

culture temp

@refgrowthtypetemperaturerange
18234positivegrowth25mesophilic
29493positivegrowth05-35
29493positiveoptimum25mesophilic
37694positivegrowth25mesophilic

culture pH

@refabilitytypepHPH range
29493positivegrowth04-10alkaliphile
29493positiveoptimum7.5-8

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
29493facultative anaerobe
121272facultative anaerobe

spore formation

@refspore formationconfidence
29493no
69481no100
69480no99.995

halophily

@refsaltgrowthtested relationconcentration
29493NaClpositivegrowth01-08 %
29493NaClpositiveoptimum01-08 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2949318403L-arabitol+carbon source
2949317057cellobiose+carbon source
2949328757fructose+carbon source
2949317234glucose+carbon source
2949317716lactose+carbon source
2949317306maltose+carbon source
2949326546rhamnose+carbon source
2949317814salicin+carbon source
2949317992sucrose+carbon source
2949327082trehalose+carbon source
2949318222xylose+carbon source
2949317632nitrate+reduction
6836925115malate+assimilation
6836917128adipate-assimilation
6836927689decanoate-assimilation
6836924265gluconate-assimilation
6836917306maltose+assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol+assimilation
6836916024D-mannose+assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose+assimilation
683695291gelatin-hydrolysis
683694853esculin+hydrolysis
6836916199urea+hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction
12127217632nitrate+reduction
12127216301nitrite-reduction

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleno
12127235581indoleno

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
18234catalase+1.11.1.6
29493catalase+1.11.1.6
29493urease+3.5.1.5
68369gelatinase-
68369beta-glucosidase+3.2.1.21
68369urease+3.5.1.5
68369arginine dihydrolase-3.5.3.6
121272oxidase+
121272catalase+1.11.1.6
121272urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase-3.1.3.2
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121272-++--+---------++---

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPAC
18234----++-++-++-+---++-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
18234seawaterPort Phillip Bay, Tasman SeaAustraliaAUSAustralia and Oceania
121272Environment, Sea waterMelbourneAustraliaAUSAustralia and Oceania2007

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic
  • Cat3: #Marine

taxonmaps

  • @ref: 69479
  • File name: preview.99_58229.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_19;96_233;97_32345;98_41461;99_58229&stattab=map
  • Last taxonomy: Celeribacter neptunius subclade
  • 16S sequence: FJ535354
  • Sequence Identity:
  • Total samples: 402
  • soil counts: 5
  • aquatic counts: 368
  • animal counts: 24
  • plant counts: 5

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
182341Risk group (German classification)
1212721Risk group (French classification)

Sequence information

16S sequences

  • @ref: 18234
  • description: Celeribacter neptunius strain H 14 16S ribosomal RNA gene, partial sequence
  • accession: FJ535354
  • length: 1454
  • database: ena
  • NCBI tax ID: 588602

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Celeribacter neptunius DSM 26471GCA_900113955scaffoldncbi588602
66792Celeribacter neptunius strain DSM 26471588602.3wgspatric588602
66792Celeribacter neptunius DSM 264712617270870draftimg588602

GC content

  • @ref: 18234
  • GC-content: 59.1

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno57.81no
flagellatedno76.648no
gram-positiveno98.63yes
anaerobicno97.418yes
aerobicyes68.983yes
halophileyes67.622no
spore-formingno96.933yes
glucose-fermentno86.919yes
thermophileno97.117yes
glucose-utilyes86.07yes

External links

@ref: 18234

culture collection no.: DSM 26471, CIP 109922, KMM 6012

straininfo link

  • @ref: 83031
  • straininfo: 370158

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny19717584Celeribacter neptunius gen. nov., sp. nov., a new member of the class Alphaproteobacteria.Ivanova EP, Webb H, Christen R, Zhukova NV, Kurilenko VV, Kalinovskaya NI, Crawford RJInt J Syst Evol Microbiol10.1099/ijs.0.014159-02009Alphaproteobacteria/*classification/genetics/isolation & purification/physiology, Base Sequence, Fatty Acids/analysis, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiologyGenetics
Phylogeny21828017Celeribacter baekdonensis sp. nov., isolated from seawater, and emended description of the genus Celeribacter Ivanova et al. 2010.Lee SY, Park S, Oh TK, Yoon JHInt J Syst Evol Microbiol10.1099/ijs.0.032227-02011Base Composition, DNA, Bacterial/genetics, Fatty Acids/metabolism, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Rhodobacteraceae/classification/genetics/*isolation & purification, Seawater/*microbiologyGenetics
Phylogeny24425746Celeribacter marinus sp. nov., isolated from coastal seawater.Baek K, Choi A, Kang I, Cho JCInt J Syst Evol Microbiol10.1099/ijs.0.060673-02014Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Molecular Sequence Data, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Rhodobacteraceae/*classification/genetics/isolation & purification, Seawater/*microbiology, Sequence Analysis, DNA, Ubiquinone/chemistryGenetics
Phylogeny25256706Celeribacter indicus sp. nov., a polycyclic aromatic hydrocarbon-degrading bacterium from deep-sea sediment and reclassification of Huaishuia halophila as Celeribacter halophilus comb. nov.Lai Q, Cao J, Yuan J, Li F, Shao ZInt J Syst Evol Microbiol10.1099/ijs.0.069039-02014Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Geologic Sediments/*microbiology, Indian Ocean, Molecular Sequence Data, Phosphatidylglycerols/chemistry, *Phylogeny, Polycyclic Aromatic Hydrocarbons/*metabolism, RNA, Ribosomal, 16S/genetics, Rhodobacteraceae/*classification/genetics/isolation & purification, Seawater/microbiology, Sequence Analysis, DNA, Ubiquinone/chemistryGenetics
Phylogeny26867899Celeribacter persicus sp. nov., a polycyclic-aromatic-hydrocarbon-degrading bacterium isolated from mangrove soil.Jami M, Lai Q, Ghanbari M, Moghadam MS, Kneifel W, Domig KJInt J Syst Evol Microbiol10.1099/ijsem.0.0009612016Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Geologic Sediments/microbiology, Indian Ocean, Iran, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Polycyclic Aromatic Hydrocarbons/*metabolism, RNA, Ribosomal, 16S/genetics, Rhodobacteraceae/*classification/genetics/isolation & purification, Sequence Analysis, DNA, *Soil Microbiology, Ubiquinone/analogs & derivatives/chemistryGenetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
18234Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 26471)https://www.dsmz.de/collection/catalogue/details/culture/DSM-26471
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
29493Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2589328776041
37694Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/7720
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68369Automatically annotated from API 20NE
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
83031Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID370158.1StrainInfo: A central database for resolving microbial strain identifiers
121272Curators of the CIPCollection of Institut Pasteur (CIP 109922)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109922