Strain identifier

BacDive ID: 1385

Type strain: Yes

Species: Paraliobacillus quinghaiensis

Strain Designation: YIM-C158

Strain history: <- X. L. Cui and Y.-G. Chen, YIM; YIM-C158

NCBI tax ID(s): 470815 (species)

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General

@ref: 7143

BacDive-ID: 1385

DSM-Number: 17857

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, moderately halophilic, chemoorganoheterotroph, spore-forming, mesophilic, Gram-positive, motile, rod-shaped, colony-forming

description: Paraliobacillus quinghaiensis YIM-C158 is an obligate aerobe, moderately halophilic, chemoorganoheterotroph bacterium that forms circular colonies and was isolated from sediment sample collected from the Dabuxun salt lake in the Qaidam Basin.

NCBI tax id

  • NCBI tax id: 470815
  • Matching level: species

strain history

  • @ref: 7143
  • history: <- X. L. Cui and Y.-G. Chen, YIM; YIM-C158

doi: 10.13145/bacdive1385.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Paraliobacillus
  • species: Paraliobacillus quinghaiensis
  • full scientific name: Paraliobacillus quinghaiensis Chen et al. 2009

@ref: 7143

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Paraliobacillus

species: Paraliobacillus quinghaiensis

full scientific name: Paraliobacillus quinghaiensis Chen et al. 2009

strain designation: YIM-C158

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityflagellum arrangementconfidence
23016positive3.0-5.0 µm0.4-0.6 µmrod-shapedyesperitrichous
69480yes97.254
69480positive100

colony morphology

  • @ref: 23016
  • colony size: 1.0-2.0 mm
  • colony color: creamy white to pale yellow
  • colony shape: circular
  • incubation period: 4-5 days
  • medium used: MMA5

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
7143BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://mediadive.dsmz.de/medium/514Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
23016MMA5yes

culture temp

@refgrowthtypetemperaturerange
7143positivegrowth30mesophilic
23016positivegrowth4.0-50.0
23016positiveoptimum37.0mesophilic

culture pH

@refabilitytypepHPH range
23016positivegrowth6.0-10.0alkaliphile
23016positiveoptimum8.0

Physiology and metabolism

oxygen tolerance

  • @ref: 23016
  • oxygen tolerance: obligate aerobe

nutrition type

  • @ref: 23016
  • type: chemoorganoheterotroph

spore formation

@refspore descriptiontype of sporespore formationconfidence
23016ellipsoidal,terminal or subterminal,in swollen sporangiaendosporeyes
69481yes97
69480yes99.999

halophily

@refhalophily levelsaltgrowthtested relationconcentration
23016moderately halophilic
23016NaClpositivegrowth1.0-20.0 %(w/v)
23016NaCloptimum5.0 %(w/v)

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
23016casein-hydrolysis
23016cellulose-hydrolysis62968
23016chitin-hydrolysis17029
23016gelatin-hydrolysis5291
23016tween 20-hydrolysis53424
23016tween 80-hydrolysis53426
23016urea-hydrolysis16199
230162-dehydro-D-gluconate+builds acid from16808
23016D-arabinose+builds acid from17108
23016maltose+builds acid from17306
23016glucose+fermentation17234
23016starch+hydrolysis28017
23016nitrate+reduction17632
23016alpha-lactose+respiration36219
23016arbutin+respiration18305
23016cellobiose+respiration17057
23016D-mannitol+respiration16899
23016D-ribose+respiration16988
23016D-xylose+respiration65327
23016glycerol+respiration17754
23016L-alanine+respiration16977
23016L-arabinose+respiration30849
23016L-asparagine+respiration17196
23016L-serine+respiration17115
23016maltose+respiration17306
23016methyl alpha-D-glucopyranoside+respiration320061
23016sucrose+respiration17992
23016trehalose+respiration27082

metabolite production

@refChebi-IDmetaboliteproduction
2301635581indoleno
2301616136hydrogen sulfideno
2301615688acetoinno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
2301615688acetoin-
2301617234glucose+

enzymes

@refvalueactivityec
23016acid phosphatase+3.1.3.2
23016alkaline phosphatase+3.1.3.1
23016alpha-glucosidase+3.2.1.20
23016beta-galactosidase+3.2.1.23
23016beta-glucosidase+3.2.1.21
23016beta-glucuronidase+3.2.1.31
23016catalase+1.11.1.6
23016cytochrome oxidase+1.9.3.1
23016esterase (C 4)+
23016esterase lipase (C 8)+
23016leucine arylamidase+3.4.11.1
23016lipase (C 14)+
23016naphthol-AS-BI-phosphohydrolase+

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentenrichment cultureenrichment culture temperature
7143sediment sample collected from the Dabuxun salt lake in the Qaidam Basinnorth-west China, Qinghai ProvinceChinaCHNAsia
23016marine agar 2216 (MA, pH 7.2; Difco) supplemented with 10 % (w/v) NaCl28.0

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Lake (large)
#Environmental#Aquatic#Sediment
#Condition#Saline

taxonmaps

  • @ref: 69479
  • File name: preview.99_35529.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_7438;97_20944;98_26219;99_35529&stattab=map
  • Last taxonomy: Paraliobacillus quinghaiensis subclade
  • 16S sequence: EU135728
  • Sequence Identity:
  • Total samples: 97
  • soil counts: 18
  • aquatic counts: 34
  • animal counts: 39
  • plant counts: 6

Safety information

risk assessment

  • @ref: 7143
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 7143
  • description: Paraliobacillus quinghaiensis strain YIM C158 16S ribosomal RNA gene, partial sequence
  • accession: EU135728
  • length: 1522
  • database: ena
  • NCBI tax ID: 470815

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Paraliobacillus quinghaiensis YIM-C158GCA_003426025contigncbi470815
66792Paraliobacillus quinghaiensis CGMCC 1.6333GCA_014645115scaffoldncbi470815
66792Paraliobacillus quinghaiensis strain CGMCC 1.6333470815.5wgspatric470815
66792Paraliobacillus quinghaiensis YIM-C1582832713448draftimg470815

GC content

  • @ref: 7143
  • GC-content: 39.5

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes97no
flagellatedyes90.234yes
gram-positiveyes87.992yes
anaerobicno94.889yes
aerobicyes56.896no
halophileyes90.296no
spore-formingyes90.788no
glucose-utilyes87.659no
thermophileno98.278yes
motileyes90.149yes
glucose-fermentno64.772yes

External links

@ref: 7143

culture collection no.: DSM 17857, CGMCC 1.6333

straininfo link

  • @ref: 71036
  • straininfo: 399372

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny19126718Paraliobacillus quinghaiensis sp. nov., isolated from salt-lake sediment in China.Chen YG, Cui XL, Zhang YQ, Li WJ, Wang YX, Xu LH, Wen ML, Peng Q, Jiang CLInt J Syst Evol Microbiol10.1099/ijs.0.65735-02009Bacillaceae/*classification/genetics/isolation & purification/physiology, Bacterial Typing Techniques, China, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Fresh Water/*microbiology, Genes, rRNA, Geologic Sediments/*microbiology, Nucleic Acid Hybridization, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Sodium Chloride, Species SpecificityPhenotype
Phylogeny22297696Alkalilactibacillus ikkensis, gen. nov., sp. nov., a novel enzyme-producing bacterium from a cold and alkaline environment in Greenland.Schmidt M, Prieme A, Johansen A, Stougaard PExtremophiles10.1007/s00792-012-0430-72012Enzymology
Phylogeny25062698Aquibacillus halophilus gen. nov., sp. nov., a moderately halophilic bacterium from a hypersaline lake, and reclassification of Virgibacillus koreensis as Aquibacillus koreensis comb. nov. and Virgibacillus albus as Aquibacillus albus comb. nov.Amoozegar MA, Bagheri M, Didari M, Mehrshad M, Schumann P, Sproer C, Sanchez-Porro C, Ventosa AInt J Syst Evol Microbiol10.1099/ijs.0.065375-02014Bacillaceae/classification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Iran, Lakes/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Salinity, Sequence Analysis, DNA, Spores, Bacterial/genetics, Virgibacillus/*classification/genetics/isolation & purification, Vitamin K 2/analogs & derivatives/chemistry, Water MicrobiologyGenetics
Phylogeny28036251Paraliobacillus sediminis sp. nov., isolated from East China sea sediment.Cao WR, Guo LY, Du ZJ, Das A, Saren G, Jiang MY, Dunlap CA, Rooney AP, Yu XK, Li TGInt J Syst Evol Microbiol10.1099/ijsem.0.0017672017Bacillaceae/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Geologic Sediments/*microbiology, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistryTranscriptome
Phylogeny34232854Paraliobacillus salinarum sp. nov., isolated from saline soil in Yingkou, China.Yin YL, Li FL, Du XY, Zhang YX, Wang LInt J Syst Evol Microbiol10.1099/ijsem.0.0048772021Bacillaceae/*classification/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, *Salinity, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistryTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
7143Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17857)https://www.dsmz.de/collection/catalogue/details/culture/DSM-17857
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
23016Yi-Guang Chen,Xiao-Long Cui,Yu-Qin Zhang,Wen-Jun Li,Yong-Xia Wang,Li-Hua Xu,Meng-Liang Wen,Qian Peng,Cheng-Lin Jiang10.1099/ijs.0.65735-0Paraliobacillus quinghaiensis sp. nov., isolated from salt-lake sediment in ChinaIJSEM 59: 28-33 200919126718
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
71036Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID399372.1StrainInfo: A central database for resolving microbial strain identifiers