Strain identifier

BacDive ID: 138440

Type strain: No

Species: Klebsiella pneumoniae

Strain Designation: B 5055

Strain history: CIP <- 1952, NCTC, Klebsiella aerogenes <- 1937, E.P. Snidjers, Amsterdam, The Netherlands: strain B 5055

NCBI tax ID(s): 573 (species)

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General

@ref: 39736

BacDive-ID: 138440

keywords: genome sequence, Bacteria, facultative anaerobe, mesophilic, Gram-negative

description: Klebsiella pneumoniae B 5055 is a facultative anaerobe, mesophilic, Gram-negative bacterium of the family Enterobacteriaceae.

NCBI tax id

  • NCBI tax id: 573
  • Matching level: species

strain history

  • @ref: 39736
  • history: CIP <- 1952, NCTC, Klebsiella aerogenes <- 1937, E.P. Snidjers, Amsterdam, The Netherlands: strain B 5055

doi: 10.13145/bacdive138440.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Enterobacteriaceae
  • genus: Klebsiella
  • species: Klebsiella pneumoniae
  • full scientific name: Klebsiella pneumoniae (Schroeter 1886) Trevisan 1887 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Hyalococcus pneumoniae

@ref: 39736

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacterales

family: Enterobacteriaceae

genus: Klebsiella

species: Klebsiella pneumoniae

strain designation: B 5055

type strain: no

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480negative99.945
39736negativerod-shapedno

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
39736MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
39736CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3
39736CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
39736positivegrowth30mesophilic
39736positivegrowth30-41
39736nogrowth5psychrophilic
39736nogrowth10psychrophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 39736
  • oxygen tolerance: facultative anaerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.864

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3973629864mannitol+fermentation
3973616947citrate+carbon source
3973617234glucose+fermentation
3973617716lactose+fermentation
3973617632nitrate+reduction
3973616301nitrite-reduction
39736132112sodium thiosulfate-builds gas from
3973617234glucose+degradation

antibiotic resistance

  • @ref: 39736
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

  • @ref: 39736
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
3973615688acetoin+
3973617234glucose-

enzymes

@refvalueactivityec
39736oxidase-
39736beta-galactosidase+3.2.1.23
39736alcohol dehydrogenase-1.1.1.1
39736gelatinase-
39736catalase+1.11.1.6
39736lysine decarboxylase+4.1.1.18
39736ornithine decarboxylase-4.1.1.17
39736phenylalanine ammonia-lyase-4.3.1.24
39736tryptophan deaminase-
39736urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
39736-+++-+----++-+-++---

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
39736+++++++++++++++++++++++++-+----++++-++-----+++--++++-++++--+++++++++-+--+--+--+++-----+--++++++-+-+

Safety information

risk assessment

  • @ref: 39736
  • biosafety level: 2
  • biosafety level comment: Risk group (French classification)

Sequence information

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Klebsiella pneumoniae subsp. pneumoniae CIP 52.145 = B5055GCA_000412575contigncbi1205678
66792Klebsiella pneumoniae strain CIP 52.145573.1488completepatric573
66792Klebsiella pneumoniae strain NCTC5055573.20128wgspatric573
66792Klebsiella pneumoniae CIP 52.1452634166475completeimg1205678

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
gram-positiveno97.405no
anaerobicno95.854no
halophileno88.127no
spore-formingno93.615no
glucose-utilyes94.578no
flagellatedno93.35no
aerobicyes90.424no
thermophileno99.521yes
motileno82.992no
glucose-fermentyes91.833no

External links

@ref: 39736

culture collection no.: CIP 52.145, NCTC 5055

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Genetics1797395Structure of the O-specific polysaccharide chain from Klebsiella pneumoniae O1K2 (NCTC 5055) lipopolysaccharide.Kol O, Wieruszeski JM, Strecker G, Montreuil J, Fournet B, Zalisz R, Smets PCarbohydr Res10.1016/0008-6215(91)84122-u1991Carbohydrate Sequence, Klebsiella pneumoniae/chemistry, Lipopolysaccharides/*chemistry, Magnetic Resonance Spectroscopy, Molecular Sequence Data, Molecular Structure
Enzymology3305533Purification of the lipopolysaccharide fraction from Klebsiella pneumoniae O1 K2 by high-performance liquid chromatography.Kol O, Montreuil J, Fournet B, Zalisz R, Smets PJ Chromatogr10.1016/s0021-9673(01)94065-81987Bacterial Proteins/analysis, Carbohydrates/analysis, Chromatography, High Pressure Liquid, Fatty Acids/analysis, Klebsiella pneumoniae/*analysis, Lipopolysaccharides/*isolation & purification, Spectrophotometry, UltravioletPhylogeny
Pathogenicity9203343Structure of the O-polysaccharide from the LPS of a Hafnia alvei strain isolated from a patient with suspect yersinosis.Karlsson C, Jansson PE, Wollin RCarbohydr Res10.1016/s0008-6215(97)00045-11997Carbohydrate Sequence, Gram-Negative Bacteria/*chemistry, Gram-Negative Bacterial Infections/*microbiology, Humans, Lipopolysaccharides/*chemistry, Molecular Sequence Data, Polysaccharides/*chemistryGenetics
15691064Immunoprotective potential of polysaccharide-tetanus toxoid conjugate in Klebsiella pneumoniae induced lobar pneumonia in rats.Chhibber S, Rani M, Vanashree YIndian J Exp Biol2005Animals, Bacterial Vaccines/administration & dosage/immunology/therapeutic use, Disease Models, Animal, Female, Klebsiella Infections/immunology/*prevention & control, Klebsiella pneumoniae/*immunology, Mice, Pneumonia, Bacterial/immunology/*prevention & control, Polysaccharides, Bacterial/administration & dosage/immunology/*therapeutic use, Rabbits, Rats, Rats, Wistar, Tetanus Toxoid/administration & dosage/immunology/*therapeutic use, Vaccination, Vaccines, Conjugate/administration & dosage/immunology/therapeutic use
Pathogenicity28586386Differential Th17 response induced by the two clades of the pandemic ST258 Klebsiella pneumoniae clonal lineages producing KPC-type carbapenemase.Clemente AM, Castronovo G, Antonelli A, D'Andrea MM, Tanturli M, Perissi E, Paccosi S, Parenti A, Cozzolino F, Rossolini GM, Torcia MGPLoS One10.1371/journal.pone.01788472017Adaptive Immunity/genetics/*immunology, Antigen-Presenting Cells/immunology, B7-2 Antigen/immunology, Bacterial Proteins/biosynthesis/*immunology, CD4-Positive T-Lymphocytes/immunology, Dendritic Cells/immunology, Genome, Bacterial, HLA-DR Antigens/immunology, Host-Pathogen Interactions/*immunology, Humans, Interleukin-17/immunology, Klebsiella Infections/genetics/*immunology/pathology, Klebsiella pneumoniae/*immunology/pathogenicity, Phylogeny, Th17 Cells/immunology, Tumor Necrosis Factor-alpha/immunology, beta-Lactamases/biosynthesis/*immunologyGenetics

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
39736Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2052.145Collection of Institut Pasteur (CIP 52.145)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1