Strain identifier
BacDive ID: 138296
Type strain:
Species: Escherichia coli
Strain Designation: RM215C
Strain history: CIP <- 1999, T.S. Whittam, Pennsylvania State Univ., Pennsylvania, USA: strain ECOR 22 <- H. Ochman and R.K. Selander: strain RM215C
NCBI tax ID(s): 562 (species)
General
@ref: 39431
BacDive-ID: 138296
keywords: Bacteria, facultative anaerobe, mesophilic, Gram-negative, motile, rod-shaped
description: Escherichia coli RM215C is a facultative anaerobe, mesophilic, Gram-negative bacterium of the family Enterobacteriaceae.
NCBI tax id
- NCBI tax id: 562
- Matching level: species
strain history
- @ref: 39431
- history: CIP <- 1999, T.S. Whittam, Pennsylvania State Univ., Pennsylvania, USA: strain ECOR 22 <- H. Ochman and R.K. Selander: strain RM215C
doi: 10.13145/bacdive138296.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Enterobacterales
- family: Enterobacteriaceae
- genus: Escherichia
- species: Escherichia coli
- full scientific name: Escherichia coli (Migula 1895) Castellani and Chalmers 1919 (Approved Lists 1980)
synonyms
- @ref: 20215
- synonym: Bacillus coli
@ref: 39431
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Enterobacterales
family: Enterobacteriaceae
genus: Escherichia
species: Escherichia coli
strain designation: RM215C
type strain: no
Morphology
cell morphology
- @ref: 39431
- gram stain: negative
- cell shape: rod-shaped
- motility: yes
colony morphology
- @ref: 39431
Culture and growth conditions
culture medium
@ref | name | growth | composition | link |
---|---|---|---|---|
39431 | MEDIUM 3 - Columbia agar | yes | Columbia agar (39.000 g);distilled water (1000.000 ml) | |
39431 | CIP Medium 3 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3 | |
39431 | CIP Medium 72 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
39431 | positive | growth | 30 | mesophilic |
39431 | positive | growth | 10-41 | |
39431 | no | growth | 5 | psychrophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 39431
- oxygen tolerance: facultative anaerobe
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
39431 | 29864 | mannitol | + | fermentation |
39431 | 16947 | citrate | - | carbon source |
39431 | 4853 | esculin | + | hydrolysis |
39431 | 17234 | glucose | + | fermentation |
39431 | 17716 | lactose | + | fermentation |
39431 | 17632 | nitrate | + | reduction |
39431 | 16301 | nitrite | + | reduction |
39431 | 15792 | malonate | - | assimilation |
39431 | 132112 | sodium thiosulfate | - | builds gas from |
39431 | 17234 | glucose | + | degradation |
antibiotic resistance
- @ref: 39431
- metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
- is antibiotic: yes
- is sensitive: no
- is resistant: yes
metabolite production
- @ref: 39431
- Chebi-ID: 35581
- metabolite: indole
- production: yes
metabolite tests
@ref | Chebi-ID | metabolite | voges-proskauer-test | methylred-test |
---|---|---|---|---|
39431 | 15688 | acetoin | - | |
39431 | 17234 | glucose | + |
enzymes
@ref | value | activity | ec |
---|---|---|---|
39431 | oxidase | - | |
39431 | beta-galactosidase | + | 3.2.1.23 |
39431 | alcohol dehydrogenase | - | 1.1.1.1 |
39431 | gelatinase | - | |
39431 | catalase | + | 1.11.1.6 |
39431 | lysine decarboxylase | + | 4.1.1.18 |
39431 | ornithine decarboxylase | - | 4.1.1.17 |
39431 | phenylalanine ammonia-lyase | - | 4.3.1.24 |
39431 | tryptophan deaminase | - | |
39431 | urease | - | 3.5.1.5 |
68382 | alkaline phosphatase | + | 3.1.3.1 |
68382 | esterase (C 4) | - | |
68382 | esterase lipase (C 8) | - | |
68382 | lipase (C 14) | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | valine arylamidase | + | |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | trypsin | - | 3.4.21.4 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | alpha-galactosidase | + | 3.2.1.22 |
68382 | beta-galactosidase | + | 3.2.1.23 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | alpha-glucosidase | + | 3.2.1.20 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
39431 | - | + | - | - | - | + | + | - | - | - | + | + | + | + | - | + | - | - | - | - |
API biotype100
@ref | GLU | FRU | GAL | TRE | MNE | SBE | MEL | SAC | RAF | MTE | MAL | LAC | LTE | MbGa | MaGa | CEL | GEN | MbGu | ESC | RIB | ARA | XYL | PLE | RHA | FUC | MLZ | DARL | LARL | XLT | DUL | TAG | GLY | INO | MAN | MTL | TUR | SOR | ADO | HBG | LYX | ERY | MDG | 3MDG | SAT | MUC | LTAT | DTAT | MTAT | DMLT | LMLT | CATE | TATE | TTE | CIT | GRT | GAT | 2KG | 5KG | TRY | NAG | GNT | PAC | PAT | pOBE | QAT | GTE | mOBE | BAT | PPAT | CMT | TGE | BET | PCE | ABT | HIN | LAT | CAP | CYT | HIS | SUC | FUM | GRE | GYT | AVT | ETN | TTN | GLN | ITA | 3OBU | APT | GTT | PRO | DALA | LALA | SER | MNT | PROP | TYR | 2KT |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
39431 | + | + | + | + | + | - | + | - | - | + | + | + | + | + | + | - | - | + | + | + | + | + | - | + | + | - | - | - | - | + | - | + | - | + | - | - | + | - | - | - | - | - | - | + | + | - | - | + | + | + | - | - | - | - | + | + | - | - | - | + | + | + | - | - | - | - | - | - | + | + | - | - | + | + | - | + | - | - | - | + | + | - | + | + | - | - | + | - | - | + | + | + | + | + | + | - | + | - | + |
Isolation, sampling and environmental information
isolation
@ref | country | origin.country | sample type | geographic location | continent |
---|---|---|---|---|---|
39431 | Bali | IDN | |||
39431 | Indonesia | IDN | Animal, Steer, healthy | Bali | Asia |
Safety information
risk assessment
- @ref: 39431
- biosafety level: 2
- biosafety level comment: Risk group (French classification)
External links
@ref: 39431
culture collection no.: CIP 106007, ECOR 22
straininfo link
- @ref: 95053
- straininfo: 68457
Reference
@id | authors | title | doi/url | catalogue |
---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
39431 | Curators of the CIP | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106007 | Collection of Institut Pasteur (CIP 106007) | |
68382 | Automatically annotated from API zym | |||
95053 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID68457.1 |