Strain identifier

BacDive ID: 138210

Type strain: Yes

Species: Cereibacter azotoformans

Strain Designation: KA25

Strain history: CIP <- 1998, JCM <- A. Hiraishi: strain KA25

NCBI tax ID(s): 43057 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 8.1 (current version)

General

@ref: 39290

BacDive-ID: 138210

keywords: 16S sequence, Bacteria, mesophilic, Gram-negative, motile, rod-shaped

description: Cereibacter azotoformans KA25 is a mesophilic, Gram-negative, motile bacterium that was isolated from Photosynthetic sludge.

NCBI tax id

  • NCBI tax id: 43057
  • Matching level: species

strain history

@refhistory
67770A. Hiraishi KA25.
39290CIP <- 1998, JCM <- A. Hiraishi: strain KA25

doi: 10.13145/bacdive138210.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Rhodobacterales
  • family: Paracoccaceae
  • genus: Cereibacter
  • species: Cereibacter azotoformans
  • full scientific name: Cereibacter azotoformans (Hiraishi et al. 1997) Hördt et al. 2020

  • synonyms

    @refsynonym
    20215Luteovulum azotoformans
    20215Rhodobacter azotoformans

@ref: 39290

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhodobacterales

family: Rhodobacteraceae

genus: Rhodobacter

species: Rhodobacter azotoformans

strain designation: KA25

type strain: yes

Morphology

cell morphology

  • @ref: 39290
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
39290MEDIUM 283 - for Rhodobacter azotoformansyesDistilled water make up to (1000.000 ml);Sodium chloride (0.200 g);Magnesium chloride hexahydrate (0.200 g);Calcium chloride dihydrate (0.050 g);Agar (20.000 g);Yeast extract (1.000 g);Ammonium sulphate (1.000 g);Potassium di-hydrogen phosphate (1.000 g);
39290CIP Medium 283yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=283

culture temp

@refgrowthtypetemperaturerange
39290positivegrowth28mesophilic
67770positivegrowth28mesophilic
39290positivegrowth15-37
39290nogrowth5psychrophilic
39290nogrowth41thermophilic

Physiology and metabolism

halophily

@refsaltgrowthtested relationconcentration
39290NaClpositivegrowth0-4 %
39290NaClnogrowth6 %
39290NaClnogrowth8 %
39290NaClnogrowth10 %

observation

  • @ref: 67770
  • observation: quinones: Q-10

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol+builds acid from18403
68371D-arabitol+builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose+builds acid from16443
68371D-lyxose+builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol+builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371salicin-builds acid from17814
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol+builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose+builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose+builds acid from30849
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
39290esculin+hydrolysis4853
39290nitrate+reduction17632
39290nitrite+reduction16301
39290glucose+degradation17234

antibiotic resistance

  • @ref: 39290
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

  • @ref: 39290
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
3929015688acetoin-
3929017234glucose-

enzymes

@refvalueactivityec
39290oxidase-
39290alcohol dehydrogenase-1.1.1.1
39290gelatinase-
39290amylase-
39290DNase-
39290caseinase-3.4.21.50
39290catalase+1.11.1.6
39290tween esterase-
39290gamma-glutamyltransferase-2.3.2.2
39290lecithinase-
39290lipase-
39290lysine decarboxylase-4.1.1.18
39290ornithine decarboxylase-4.1.1.17
39290phenylalanine ammonia-lyase-4.3.1.24
39290tryptophan deaminase-
39290urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase-3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
39290-+++-+-----+---+----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
39290--+/-+++---+/-+++----+------+/--+/-+/----------+--++--++---

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
39290-----------+----+-----------------------------------------------------------------------+----------

Isolation, sampling and environmental information

isolation

@refsample type
67770Photosynthetic sludge
39290Environment, Photosynthetic sludge

taxonmaps

  • @ref: 69479
  • File name: preview.99_4870.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_19;96_1024;97_1194;98_3646;99_4870&stattab=map
  • Last taxonomy: Luteovulum azotoformans subclade
  • 16S sequence: AB607332
  • Sequence Identity:
  • Total samples: 975
  • soil counts: 147
  • aquatic counts: 520
  • animal counts: 288
  • plant counts: 20

Safety information

risk assessment

  • @ref: 39290
  • biosafety level: 1
  • biosafety level comment: Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
67770Rhodobacter azotoformans gene for 16S rRNA, partial sequenceAB6073321418ena43057
67770Rhodobacter azotoformans (strain KA25) gene for 16S rRNAD708461389ena43057

GC content

  • @ref: 67770
  • GC-content: 70
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 39290

culture collection no.: CIP 105439, JCM 9340, IAM 14814, IFO 16436, NBRC 16436

straininfo link

  • @ref: 94983
  • straininfo: 62690

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Enzymology12679539"Green-like" and "red-like" RubisCO cbbL genes in Rhodobacter azotoformans.Uchino Y, Yokota AMol Biol Evol10.1093/molbev/msg1002003Base Sequence, Blotting, Southern, Cluster Analysis, Codon/genetics, DNA Primers, Electrophoresis, *Evolution, Molecular, Likelihood Functions, Molecular Sequence Data, *Phylogeny, Rhodobacter/*genetics, Ribulose-Bisphosphate Carboxylase/*genetics, Sequence Analysis, DNAGenetics
Phylogeny18523181Rhodobacter ovatus sp. nov., a phototrophic alphaproteobacterium isolated from a polluted pond.Srinivas TN, Anil Kumar P, Sasikala Ch, Sproer C, Ramana ChVInt J Syst Evol Microbiol10.1099/ijs.0.65619-02008Bacterial Typing Techniques, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Fresh Water/*microbiology, Genes, rRNA, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, *Phototrophic Processes, Phylogeny, RNA, Ribosomal, 16S/genetics, Rhodobacter/*classification/genetics/growth & development/*isolation & purification, Sequence Analysis, DNA, Species Specificity, *Water PollutionGenetics

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
39290Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105439Collection of Institut Pasteur (CIP 105439)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
94983Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID62690.1