Strain identifier

BacDive ID: 138104

Type strain: No

Species: Nocardia pseudosporangifera

Strain Designation: 2-41, A-0290

Strain history: CIP <- 1996, JCM <- KCC: strain A-0290 <- IAM <- N. Seto and H. Iizuka: strain 2-41

NCBI tax ID(s): 53430 (species)

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General

@ref: 39112

BacDive-ID: 138104

keywords: obligate aerobe, mesophilic, Gram-positive, rod-shaped

description: Nocardia pseudosporangifera 2-41 is an obligate aerobe, mesophilic, Gram-positive prokaryote of the family Not assigned to family.

NCBI tax id

  • NCBI tax id: 53430
  • Matching level: species

strain history

@refhistory
67770KCC A-0290 <-- IAM 503 <-- N. Seto and H. Iizuka 2-41.
39112CIP <- 1996, JCM <- KCC: strain A-0290 <- IAM <- N. Seto and H. Iizuka: strain 2-41

doi: 10.13145/bacdive138104.20241212.9.2

Name and taxonomic classification

@ref: 39112

phylum: Not assigned to order

class: Not assigned to order

order: Not assigned to order

family: Not assigned to family

genus: Nocardia

species: Nocardia pseudosporangifera

strain designation: 2-41, A-0290

type strain: no

Morphology

cell morphology

  • @ref: 39112
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 39112

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
39112MEDIUM 146 - for Nocardia farcinica and Nocardia pseudosporangiferayesDistilled water make up to (1000.000 ml);Sodium chloride (5.000 g);Agar(15.000 g);Glucose (10.000 g);Yeast extract (2.000 g);Beef extract (1.000 g)
39112CIP Medium 146yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=146

culture temp

@refgrowthtypetemperature
39112positivegrowth30
67770positivegrowth28
39112positivegrowth25-41
39112nogrowth10
39112nogrowth45

Physiology and metabolism

oxygen tolerance

  • @ref: 39112
  • oxygen tolerance: obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
39112NaClpositivegrowth0-6 %
39112NaClnogrowth8 %
39112NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3911216947citrate-carbon source
391124853esculin-hydrolysis
39112606565hippurate+hydrolysis
3911217632nitrate+reduction
3911216301nitrite-reduction
3911217632nitrate-respiration
68371Potassium 5-ketogluconate+builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837112936D-galactose+builds acid from
6837117754glycerol-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose-builds acid from
6837117992sucrose-builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117306maltose-builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol+builds acid from
6837116813galactitol+builds acid from
6837162345L-rhamnose+builds acid from
6837117266L-sorbose+builds acid from
6837116024D-mannose+builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose+builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose-builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from

antibiotic resistance

  • @ref: 39112
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

  • @ref: 39112
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
3911215688acetoin-
3911217234glucose-

enzymes

@refvalueactivityec
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382beta-glucuronidase-3.2.1.31
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-chymotrypsin-3.4.21.1
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382esterase lipase (C 8)+
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
39112oxidase-
39112beta-galactosidase+3.2.1.23
39112alcohol dehydrogenase-1.1.1.1
39112gelatinase+/-
39112amylase-
39112DNase-
39112caseinase-3.4.21.50
39112catalase+1.11.1.6
39112tween esterase-
39112gamma-glutamyltransferase-2.3.2.2
39112lecithinase-
39112lipase-
39112lysine decarboxylase-4.1.1.18
39112ornithine decarboxylase-4.1.1.17
39112phenylalanine ammonia-lyase-4.3.1.24
39112protease-
39112tryptophan deaminase-
39112urease-3.5.1.5
68382alpha-fucosidase-3.2.1.51
68382acid phosphatase+3.1.3.2
68382lipase (C 14)+

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
39112--++++----++-+-++---

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
39112---------++++++++-------+/------------------------+

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
39112+------------------------------------------------+------------------------++--+++-------+-+-----+++

Isolation, sampling and environmental information

isolation

@refgeographic locationcountryorigin.countrycontinentsample type
39112NiigataJapanJPNAsia
67770Nishiyama oil field, Niigata Pref.JapanJPNAsiaSoil
39112NiigataJapanJPNAsiaEnvironment, Soil

Safety information

risk assessment

  • @ref: 39112
  • biosafety level: 2
  • biosafety level comment: Risk group (French classification)

External links

@ref: 39112

culture collection no.: CIP 104827, IAM 0503, JCM 3290, IAM 503

Reference

@idauthorscataloguedoi/urltitle
39112Curators of the CIPCollection of Institut Pasteur (CIP 104827)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104827
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym