Strain identifier

BacDive ID: 13789

Type strain: Yes

Species: Sagittula stellata

Strain Designation: E 37, E-37

Strain history: CIP <- 1997, J. Gonzales, Univ. Georgia, Atlanta, GA, USA: strain E-37

NCBI tax ID(s): 52603 (species)

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General

@ref: 4399

BacDive-ID: 13789

DSM-Number: 11524

keywords: 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-negative, rod-shaped

description: Sagittula stellata E 37 is an obligate aerobe, mesophilic, Gram-negative bacterium that was isolated from enrichment community growing on the high molecular weight fraction of a pulp mill effluent, inoculated from seawater off the coast of Georgia.

NCBI tax id

  • NCBI tax id: 52603
  • Matching level: species

strain history

@refhistory
4399<- J. M. Gonzáles, E37
123304CIP <- 1997, J. Gonzales, Univ. Georgia, Atlanta, GA, USA: strain E-37

doi: 10.13145/bacdive13789.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Rhodobacterales
  • family: Roseobacteraceae
  • genus: Sagittula
  • species: Sagittula stellata
  • full scientific name: Sagittula stellata Gonzalez et al. 1997

@ref: 4399

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhodobacterales

family: Rhodobacteraceae

genus: Sagittula

species: Sagittula stellata

full scientific name: Sagittula stellata Gonzalez et al. 1997

strain designation: E 37, E-37

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
43369negative2.3 µm0.9 µmrod-shapedno
123304negativerod-shapedyes

colony morphology

@refincubation periodcolony sizecolony colorcolony shapemedium used
43992-3 days
433690.5 mmcream coloredcircularcomplex media

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
4399BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://mediadive.dsmz.de/medium/514Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
39235Marine agar (MA)yesDistilled water make up to (1000.000 ml);Marine agar (55.100 g)
43369Shioi`s marine mediumyes
43369Tryptic soy agarno
43369complex mediayes
123304CIP Medium 13yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13

culture temp

@refgrowthtypetemperaturerange
4399positivegrowth28mesophilic
39235positivegrowth30mesophilic
43369positivegrowth4-41
123304positivegrowth25-37mesophilic
123304nogrowth5psychrophilic
123304nogrowth10psychrophilic
123304nogrowth41thermophilic

culture pH

@refabilitytypepHPH range
43369positivegrowth5.5-8.5alkaliphile
43369nogrowth9.5
43369positiveoptimum7.5

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
43369obligate aerobe
123304obligate aerobe

observation

  • @ref: 43369
  • observation: absolute requirement for NaCl, failed to grow when NaCl was replaced with with KCl or LiCl

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4336985146carboxymethylcellulose+hydrolysis
4336962968cellulose+hydrolysis
6836925115malate+assimilation
6836927689decanoate-assimilation
6836924265gluconate+assimilation
6836917306maltose+assimilation
6836959640N-acetylglucosamine+assimilation
6836916899D-mannitol+assimilation
6836916024D-mannose+assimilation
6836930849L-arabinose+assimilation
6836917634D-glucose+assimilation
683695291gelatin-hydrolysis
683694853esculin+hydrolysis
6836916199urea+hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction
1233044853esculin-hydrolysis
12330417632nitrate-reduction
12330416301nitrite-reduction
12330417632nitrate-respiration

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitiveis resistant
4336928971ampicillinyesyes
4336917698chloramphenicolyesyes
433696104kanamycinyesyes
4336918208penicillin gyesyes
4336917076streptomycinyesyes
4336927902tetracyclineyesyes
4336928001vancomycinyesyes
1233040129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)yesyesno

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleno
12330435581indoleno

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
4399catalase+1.11.1.6
4399cytochrome-c oxidase+1.9.3.1
68369cytochrome oxidase+1.9.3.1
68369gelatinase-
68369beta-glucosidase+3.2.1.21
68369urease+3.5.1.5
68369arginine dihydrolase-3.5.3.6
123304oxidase+
123304beta-galactosidase+3.2.1.23
123304alcohol dehydrogenase-1.1.1.1
123304gelatinase-
123304amylase-
123304DNase-
123304caseinase-3.4.21.50
123304catalase+1.11.1.6
123304tween esterase-
123304lecithinase-
123304lipase-
123304lysine decarboxylase-4.1.1.18
123304ornithine decarboxylase-4.1.1.17
123304protease-
123304tryptophan deaminase-
123304urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123304-+++-+----++---+----

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
4399----++--+++++++-+/-++++

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
123304-+-+---+--+----+------+---+----+-+-++-----------++---------+------+--------+---++-------+--+------+

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
4399enrichment community growing on the high molecular weight fraction of a pulp mill effluent, inoculated from seawater off the coast of GeorgiaGeorgiaUSAUSANorth America
123304Environment, Coastal seawaterGeorgiaUnited States of AmericaUSANorth America1994

isolation source categories

Cat1Cat2Cat3
#Engineered#Industrial#Industrial production
#Engineered#Laboratory#Lab enrichment
#Engineered#Waste#Wastewater
#Environmental#Microbial community

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
43991Risk group (German classification)
1233041Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Sagittula stellata strain DSM11524 16S ribosomal RNA gene, partial sequenceDQ9156281294ena52603
4399Lignin enrichment culture E-37 16S ribosomal RNA gene, partial sequenceU583561373ena52603

GC content

  • @ref: 43369
  • GC-content: 65
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 4399

culture collection no.: DSM 11524, ATCC 700073, CIP 105237, CIP 105724

straininfo link

  • @ref: 82967
  • straininfo: 43756

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny9226910Sagittula stellata gen. nov., sp. nov., a lignin-transforming bacterium from a coastal environment.Gonzalez JM, Mayer F, Moran MA, Hodson RE, Whitman WBInt J Syst Bacteriol10.1099/00207713-47-3-7731997Bacterial Adhesion/physiology, Carbohydrate Metabolism, Cell Division, Cellulose/metabolism, DNA, Bacterial/analysis, *Environmental Microbiology, Gram-Negative Bacteria/*classification/metabolism/ultrastructure, Lignin/*metabolism, Microscopy, Electron, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/analysis, Seawater/*microbiologyMetabolism
Phylogeny23087164Litorisediminicola beolgyonensis gen. nov., sp. nov., isolated from a coastal sediment.Yoon JH, Lee SY, Jung YT, Lee JS, Lee KCInt J Syst Evol Microbiol10.1099/ijs.0.043588-02012Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/analysis, Genes, Bacterial, Geologic Sediments/*microbiology, Molecular Sequence Data, Phospholipids/analysis, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Rhodobacteraceae/*classification/genetics/isolation & purification, Seawater/*microbiology, Sequence Analysis, DNA, Ubiquinone/analysis, Water MicrobiologyGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
4399Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 11524)https://www.dsmz.de/collection/catalogue/details/culture/DSM-11524
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
39235Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17235
43369J. M. Gonzalez, F. Mayer, M. A. Moran, R. E. Hodson, W. B. Whitman10.1099/00207713-47-3-773Sagittula stellata gen. nov., sp. nov., a lignin transforming bacterium from a coastal environmentIJSB 47: 773-780 19979226910
68369Automatically annotated from API 20NE
68382Automatically annotated from API zym
82967Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID43756.1StrainInfo: A central database for resolving microbial strain identifiers
123304Curators of the CIPCollection of Institut Pasteur (CIP 105237)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105237